-
公开(公告)号:US10228375B2
公开(公告)日:2019-03-12
申请号:US15629634
申请日:2017-06-21
摘要: Water soluble light harvesting multichromophores that have an ultraviolet absorption maximum are provided. In some embodiments, the multichromophores include a conjugated segment including a fused 6-5-6 tricyclic co-monomer and a UV absorbance-modifying co-monomer. The multichromophores may include an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. In some embodiments, a specific binding member is covalently linked to the multichromophore. Also provided are methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule using compositions including the light harvesting multichromophores. Kits and systems for practicing the subject methods are also provided.
-
公开(公告)号:US20180163054A1
公开(公告)日:2018-06-14
申请号:US15837959
申请日:2017-12-11
IPC分类号: C09B69/10 , G01N33/533
摘要: Water soluble polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers including branched non-ionic water soluble groups (WSG) comprising two or more water soluble polymers. In some cases, the branched non-ionic water soluble groups (WSG) of the present disclosure are capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are aggregation-resistant labelled specific binding members that include the subject water soluble polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US20240294768A1
公开(公告)日:2024-09-05
申请号:US18627172
申请日:2024-04-04
IPC分类号: C09B69/10 , G01N33/533
CPC分类号: C09B69/109 , C09B69/10 , C09B69/101 , G01N33/533
摘要: Water soluble polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers including branched non-ionic water soluble groups (WSG) comprising two or more water soluble polymers. In some cases, the branched non-ionic water soluble groups (WSG) of the present disclosure are capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are aggregation-resistant labelled specific binding members that include the subject water soluble polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US20240240025A1
公开(公告)日:2024-07-18
申请号:US18411706
申请日:2024-01-12
发明人: Yongchao Liang , Haiqing Li , Brent S. Gaylord
CPC分类号: C09B69/10 , C08G61/124 , C08G61/126 , C09B69/00 , C08G2261/1424 , C08G2261/148 , C08G2261/1644 , C08G2261/1646 , C08G2261/3142 , C08G2261/3223 , C08G2261/3241 , C08G2261/3243 , C08G2261/3246 , C08G2261/344 , C08G2261/41 , C08G2261/411 , C08G2261/414 , C08G2261/522 , C08G2261/72 , G01N33/582
摘要: Water solvated polymeric dyes and polymeric tandem dyes having a blue excitation spectrum are provided. The polymeric dyes are conjugated polymers that can include a thiophene-containing co-monomer. The polymeric tandem dyes further include a signaling chromophore covalently linked to the conjugated polymer in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US11977078B2
公开(公告)日:2024-05-07
申请号:US18139747
申请日:2023-04-26
CPC分类号: G01N33/582 , C08G61/02 , C08G2261/124 , C08G2261/1424 , C08G2261/148 , C08G2261/1644 , C08G2261/312 , C08G2261/3142
摘要: Water soluble light harvesting multichromophores that have an ultraviolet absorption maximum are provided. In some embodiments, the multichromophores include a conjugated segment including a fused 6-5-6 tricyclic co-monomer and a UV absorbance-modifying co-monomer. The multichromophores may include an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. In some embodiments, a specific binding member is covalently linked to the multichromophore. Also provided are methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule using compositions including the light harvesting multichromophores. Kits and systems for practicing the subject methods are also provided.
-
公开(公告)号:US11732138B2
公开(公告)日:2023-08-22
申请号:US17955087
申请日:2022-09-28
发明人: Yongchao Liang , Jonathan Hollinger , Brian Wall , David Moureau , Lucy Zhao , Glenn P. Bartholomew
IPC分类号: C09B69/10 , C08G61/10 , C08G61/12 , C09B69/00 , G01N33/58 , G01N33/542 , G01N33/533
CPC分类号: C09B69/109 , C08G61/10 , C08G61/12 , C09B69/00 , C09B69/101 , C09B69/102 , G01N33/582 , C08G2261/135 , C08G2261/143 , C08G2261/145 , C08G2261/1424 , C08G2261/1452 , C08G2261/1644 , C08G2261/312 , C08G2261/334 , C08G2261/344 , C08G2261/522 , C08G2261/90 , G01N33/533 , G01N33/542
摘要: Water solvated polymeric dyes having a deep ultraviolet excitation spectrum are provided. The subject polymeric dyes include a light harvesting multichromophore having conjugation-modifying repeat units incorporated into the polymer backbone to provide segments of π-conjugated co-monomers having limited π-conjugation between segments. Polymeric tandem dyes are also provided that further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject water solvated polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US20230235177A1
公开(公告)日:2023-07-27
申请号:US17955087
申请日:2022-09-28
发明人: Yongchao Liang , Jonathan Hollinger , Brian Wall , David Moureau , Lucy Zhao , Glenn P. Bartholomew
CPC分类号: C09B69/109 , C08G61/10 , C08G61/12 , C09B69/101 , C09B69/00 , C09B69/102 , G01N33/582 , C08G2261/135 , C08G2261/1424 , C08G2261/1452 , G01N33/542
摘要: Water solvated polymeric dyes having a deep ultraviolet excitation spectrum are provided. The subject polymeric dyes include a light harvesting multichromophore having conjugation-modifying repeat units incorporated into the polymer backbone to provide segments of π-conjugated co-monomers having limited π-conjugation between segments. Polymeric tandem dyes are also provided that further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject water solvated polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US11702547B2
公开(公告)日:2023-07-18
申请号:US17531067
申请日:2021-11-19
发明人: Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
CPC分类号: C09B69/109 , G01N33/582
摘要: Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US11499053B2
公开(公告)日:2022-11-15
申请号:US17316508
申请日:2021-05-10
IPC分类号: C09B69/10 , G01N33/533
摘要: Water soluble polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers including branched non-ionic water soluble groups (WSG) comprising two or more water soluble polymers. In some cases, the branched non-ionic water soluble groups (WSG) of the present disclosure are capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are aggregation-resistant labelled specific binding members that include the subject water soluble polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
公开(公告)号:US10920082B2
公开(公告)日:2021-02-16
申请号:US15642255
申请日:2017-07-05
发明人: Yongchao Liang , Haiqing Li , Brent S. Gaylord
摘要: Water solvated polymeric dyes and polymeric tandem dyes having a blue excitation spectrum are provided. The polymeric dyes are conjugated polymers that can include a thiophene-containing co-monomer. The polymeric tandem dyes further include a signaling chromophore covalently linked to the conjugated polymer in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
-
-
-
-
-
-
-
-
-