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公开(公告)号：US20230295441A1
公开(公告)日：2023-09-21
申请号：US18201649
申请日：2023-05-24
发明人： Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
CPC分类号： C09B69/109 , G01N33/582
摘要： Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20230152327A1
公开(公告)日：2023-05-18
申请号：US17876349
申请日：2022-07-28
CPC分类号： G01N33/582 , C09B23/0075
摘要： Water-soluble dipyrromethene-based dyes are provided herein. In certain cases, the water-soluble dye is a dipyrromethene-boron dye. Embodiments of the subject water-soluble dipyrromethene-based dyes have a narrow absorption maxima in the yellow green range close to or at the 561 nm laser line, and are suitable for use in tandem dye systems. Embodiments of the subject dyes efficiently transfer energy to an acceptor chromophore while a second dipyrromethene-based dye of the same or different chemical structure is within energy-receiving proximity therewith. Also provided herein are tandem dyes that include the subject water-soluble dipyrromethene-based dyes, e.g., as donor chromophores. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US11492493B2
公开(公告)日：2022-11-08
申请号：US16226381
申请日：2018-12-19
发明人： Yongchao Liang , Jonathan Hollinger , Brian Wall , David Moureau , Lucy Zhao , Glenn P. Bartholomew
IPC分类号： C09B69/10 , C08G61/10 , C08G61/12 , C09B69/00 , G01N33/58 , G01N33/542 , G01N33/533
摘要： Water solvated polymeric dyes having a deep ultraviolet excitation spectrum are provided. The subject polymeric dyes include a light harvesting multichromophore having conjugation-modifying repeat units incorporated into the polymer backbone to provide segments of π-conjugated co-monomers having limited π-conjugation between segments. Polymeric tandem dyes are also provided that further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject water solvated polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US11214688B2
公开(公告)日：2022-01-04
申请号：US17003591
申请日：2020-08-26
发明人： Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
摘要： Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US10604657B2
公开(公告)日：2020-03-31
申请号：US15642216
申请日：2017-07-05
摘要： Water solvated polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers linked via covalent bonds, vinylene groups or ethynylene groups. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labeled specific binding members that include the subject polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labeling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20190194467A1
公开(公告)日：2019-06-27
申请号：US16226381
申请日：2018-12-19
发明人： Yongchao Liang , Jonathan Hollinger , Brian Wall , David Moureau , Lucy Zhao , Glenn P. Bartholomew
CPC分类号： C09B69/109 , C08G61/10 , C08G2261/135 , C08G2261/1424 , C08G2261/1452 , C08G2261/1644 , C08G2261/334 , C08G2261/522 , C08G2261/90 , G01N33/533 , G01N33/542
摘要： Water solvated polymeric dyes having a deep ultraviolet excitation spectrum are provided. The subject polymeric dyes include a light harvesting multichromophore having conjugation-modifying repeat units incorporated into the polymer backbone to provide segments of π-conjugated co-monomers having limited π-conjugation between segments. Polymeric tandem dyes are also provided that further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject water solvated polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20180009990A1
公开(公告)日：2018-01-11
申请号：US15642216
申请日：2017-07-05
CPC分类号： C09B69/105 , C08G61/02 , C08G61/10 , C08G61/12 , C08G61/123 , C08G61/124 , C08G61/126 , C08G2261/12 , C08G2261/124 , C08G2261/1424 , C08G2261/1432 , C08G2261/146 , C08G2261/148 , C08G2261/312 , C08G2261/314 , C08G2261/3142 , C08G2261/3241 , C08G2261/3242 , C08G2261/3245 , C08G2261/3327 , C08G2261/3328 , C08G2261/3422 , C08G2261/522 , C09B69/00 , C09B69/101 , C09B69/102 , C09B69/109 , G01N33/582
摘要： Water solvated polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers linked via covalent bonds, vinylene groups or ethynylene groups. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labeled specific binding members that include the subject polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labeling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20170285039A1
公开(公告)日：2017-10-05
申请号：US15629634
申请日：2017-06-21
CPC分类号： G01N33/582 , C08G61/02 , C08G2261/124 , C08G2261/1424 , C08G2261/148 , C08G2261/1644 , C08G2261/312 , C08G2261/3142
摘要： Water soluble light harvesting multichromophores that have an ultraviolet absorption maximum are provided. In some embodiments, the multichromophores include a conjugated segment including a fused 6-5-6 tricyclic co-monomer and a UV absorbance-modifying co-monomer. The multichromophores may include an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. In some embodiments, a specific binding member is covalently linked to the multichromophore. Also provided are methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule using compositions including the light harvesting multichromophores. Kits and systems for practicing the subject methods are also provided.
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公开(公告)号：US11987710B2
公开(公告)日：2024-05-21
申请号：US17956044
申请日：2022-09-29
IPC分类号： C09B69/10 , G01N33/533
CPC分类号： C09B69/109 , C09B69/10 , C09B69/101 , G01N33/533
摘要： Water soluble polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers including branched non-ionic water soluble groups (WSG) comprising two or more water soluble polymers. In some cases, the branched non-ionic water soluble groups (WSG) of the present disclosure are capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are aggregation-resistant labelled specific binding members that include the subject water soluble polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US11668716B2
公开(公告)日：2023-06-06
申请号：US17400766
申请日：2021-08-12
CPC分类号： G01N33/582 , C08G61/02 , C08G2261/124 , C08G2261/148 , C08G2261/1424 , C08G2261/1644 , C08G2261/312 , C08G2261/3142
摘要： Water soluble light harvesting multichromophores that have an ultraviolet absorption maximum are provided. In some embodiments, the multichromophores include a conjugated segment including a fused 6-5-6 tricyclic co-monomer and a UV absorbance-modifying co-monomer. The multichromophores may include an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. In some embodiments, a specific binding member is covalently linked to the multichromophore. Also provided are methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule using compositions including the light harvesting multichromophores. Kits and systems for practicing the subject methods are also provided.
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