Abstract:
Provided are a composition for preparing D-psicose comprising a microorganism of the genus Kaistia, and a method for preparing D-psicose using the same.
Abstract:
The present disclosure relates to fructose-6-phosphate-3-epimerase consisting of an amino acid sequence of SEQ ID NO: 1, a nucleic acid encoding the fructose-6-phosphate-3-epimerase, and a transformant comprising the nucleic acid. Additionally, the present disclosure relates to a composition for producing allulose, which comprises the fructose-6-phosphate-3-epimerase of the present disclosure, and a method for producing allulose using the fructose-6-phosphate-3-epimerase of the present disclosure.
Abstract:
The present invention relates to a method for preparing tagatose from residue after extracting coffee, comprising: obtaining a hydrolysate by hydrolyzing residue after extracting coffee into acid; obtaining a hydrolysate refined by decolorization and ion-refining the obtained hydrolysate; and obtaining tagatose by isomerizing the refined hydrolysate to an arabinose isomerase.
Abstract:
A method for producing tagatose comprises: a) performing epimerization of fructose using hexuronate C4-epimerase to obtain an epimerized product comprising tagatose; b) purifying the epimerized product; and c) crystallizing the purified epimerized product. The hexuronate C4-epimerase is an enzyme derived from Thermotoga maritima, Thermotoga neapolitana, Thermotoga thermarum or mutants thereof. The hexuronate C4-epimerase is produced from strains Escherichia. coli, Corynebacterum glutamicum, Aspergillus oryzae, or Bacillus subtilis.