公开(公告)号：US20020013286A1

公开(公告)日：2002-01-31

申请号：US09835683

申请日：2001-04-16

Applicant: GenVec, Inc.

Inventor： Joseph T. Bruder ,  Imre Kovesdi

IPC: A61K048/00 ,  A61K039/395

CPC classification number: A61K48/00 ,  A61K2039/505 ,  C07K16/081

Abstract: The present invention provides methods for administering an adenoviral gene transfer vector comprising an exogenous gene to an animal. One method involves utilizing systemic neutralizing antibodies to neutralize the adenoviral gene transfer vector outside a targeted muscle. Another method involves the repeat administration of an adenoviral gene transfer vector to a skeletal muscle.

Abstract translation: 本发明提供了向动物施用包含外源基因的腺病毒基因转移载体的方法。 一种方法涉及利用全身中和抗体来中和靶向肌肉外的腺病毒基因转移载体。 另一种方法涉及向骨骼肌重复施用腺病毒基因转移载体。

公开(公告)号：US20010026794A1

公开(公告)日：2001-10-04

申请号：US09780526

申请日：2001-02-09

Applicant: GenVec, Inc.

Inventor： Imre Kovesdi ,  Duncan L. McVey ,  Thomas J. Wickham ,  Joseph T. Bruder ,  Douglas E. Brough

IPC: A61K048/00 ,  C12Q001/70 ,  C12N007/01

CPC classification number: C12N15/1093 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40

Abstract: The present invention provides a library of viral vectors, wherein each member comprises a first heterologous DNA encoding a first gene product and a second heterologous DNA encoding a second gene product. The first heterologous DNA is common to each member of the library, while the second heterologous DNA varies between members of the library. The present invention additionally provides a method of constructing a library of viral vectors. The method comprises carrying out homologous recombination between a first DNA molecule and a second DNA molecule to form a pool of intermediate viral vector genomes. One or more linear third DNA molecules are ligated into the pool of intermediate viral genomes to produce a library of viral vector genomes. Alternatively, homologous recombination between linear DNA molecules and recipient DNA molecules produces a library of viral vector genomes. The library of viral vector genomes is converted into a library of viral vectors.

Abstract translation: 本发明提供病毒载体文库，其中每个成员包含编码第一基因产物的第一异源DNA和编码第二基因产物的第二异源DNA。 第一个异源DNA对于文库的每个成员是共同的，而第二异源DNA在文库的成员之间是不同的。 本发明另外提供构建病毒载体文库的方法。 该方法包括在第一DNA分子和第二DNA分子之间进行同源重组以形成中间病毒载体基因组池。 将一个或多个线性第三DNA分子连接到中间病毒基因组池中以产生病毒载体基因组文库。 或者，线性DNA分子和受体DNA分子之间的同源重组产生病毒载体基因组文库。 将病毒载体基因组文库转化为病毒载体文库。