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35 条记录 (耗时 0.051 秒)

    Detection and Quantitation of Nucleic Acid Molecules in Biological Samples
    32.
    发明申请
    Detection and Quantitation of Nucleic Acid Molecules in Biological Samples 有权
    生物样品中核酸分子的检测和定量

    公开(公告)号：US20080050721A1

    公开(公告)日：2008-02-28

    申请号：US10568134

    申请日：2004-08-17

    申请人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

    发明人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  Y10T436/143333 ,  C12Q2545/114

    摘要： The present invention concerns processes for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridizationdetection assays, antibody-mediated recognition assays, nucleic acid sensor molecules, chromatographic assays, and/or electrophoresis assays. The present invention specifically concerns processes for the detection and quantitation of double stranded nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridization-detection assays. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include molecules that mediate RNA interference, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include nucleic acid aptamers, enzymatic nucleic acid molecules, decoys, antisense, 2,5′-oligoadenylate molecules, triplex forming oligonucleotides or any other nucleic acid molecule of interest. The present invention also concerns kits that allow for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample.

    摘要翻译： 本发明涉及使用杂交检测测定，抗体介导的识别测定，核酸传感器分子，色谱测定和/或电泳测定来检测和定量样品中核酸分子，多核苷酸和/或寡核苷酸的方法。 本发明具体涉及使用杂交检测试验检测和定量样品中双链核酸分子，多核苷酸和/或寡核苷酸的方法。 核酸分子，多核苷酸和/或寡核苷酸可以包括介导RNA干扰的分子，例如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA） ，和短发夹RNA（shRNA）分子。 核酸分子，多核苷酸和/或寡核苷酸可以包括核酸适体，酶核酸分子，诱饵，反义，2,5'-寡腺苷酸分子，形成三链体的寡核苷酸或任何其它感兴趣的核酸分子。 本发明还涉及允许在样品中检测和定量核酸分子，多核苷酸和/或寡核苷酸的试剂盒。

    Detection and quantitation of nucleic acid molecules in biological samples
    33.
    发明授权
    Detection and quantitation of nucleic acid molecules in biological samples 有权
    检测和定量生物样品中的核酸分子

    公开(公告)号：US07521184B2

    公开(公告)日：2009-04-21

    申请号：US10568134

    申请日：2004-08-17

    申请人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

    发明人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  Y10T436/143333 ,  C12Q2545/114

    摘要： The present invention concerns processes for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridizationdetection assays, antibody-mediated recognition assays, nucleic acid sensor molecules, chromatographic assays, and/or electrophoresis assays. The present invention specifically concerns processes for the detection and quantitation of double stranded nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridization-detection assays. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include molecules that mediate RNA interference, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include nucleic acid aptamers, enzymatic nucleic acid molecules, decoys, antisense, 2′,5′-oligoadenylate molecules, triplex forming oligonucleotides or any other nucleic acid molecule of interest. The present invention also concerns kits that allow for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample.

    摘要翻译： 本发明涉及使用杂交检测测定，抗体介导的识别测定，核酸传感器分子，色谱测定和/或电泳测定来检测和定量样品中核酸分子，多核苷酸和/或寡核苷酸的方法。 本发明具体涉及使用杂交检测试验检测和定量样品中双链核酸分子，多核苷酸和/或寡核苷酸的方法。 核酸分子，多核苷酸和/或寡核苷酸可以包括介导RNA干扰的分子，例如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA） ，和短发夹RNA（shRNA）分子。 核酸分子，多核苷酸和/或寡核苷酸可以包括核酸适配体，酶核酸分子，诱饵，反义，2'，5'-寡聚腺苷酸分子，形成三链体的寡核苷酸或任何其它感兴趣的核酸分子。 本发明还涉及允许在样品中检测和定量核酸分子，多核苷酸和/或寡核苷酸的试剂盒。

    RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
    35.
    发明申请
    RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA) 审中-公开
    RNA干扰介导使用短干扰核酸（siNA）抑制基因表达

    公开(公告)号：US20070270579A1

    公开(公告)日：2007-11-22

    申请号：US11234730

    申请日：2005-09-23

    申请人： Vasant Jadhav ,  Narendra Vaish

    发明人： Vasant Jadhav ,  Narendra Vaish

    IPC分类号： C07H21/04

    CPC分类号： C07H21/04

    摘要： This invention relates to compounds, compositions, and methods useful for modulating gene expression using short interfering nucleic acid (siNA) molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of genes, such as expressed pseudogenes associated with the maintenance or development of diseases, disorders, traits, and conditions in a subject or organism. The invention also provides small nucleic acid molecules with reduced or attenuated immunostimulatory properties and methods for designing and synthesizing such small nucleic acid molecules having improved toxicologic properties while retaining RNAi activity.

    摘要翻译： 本发明涉及使用短干扰核酸（siNA）分子调节基因表达的化合物，组合物和方法。 特别地，本发明的特征在于小核酸分子，例如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA）和短发夹RNA（shRNA） ）分子和用于调节基因表达的方法，例如与受试者或生物中的疾病，病症，性状和病症的维持或发展相关的表达的假基因。 本发明还提供具有降低或减弱的免疫刺激性质的小核酸分子以及用于设计和合成具有改善的毒理学特性同时保留RNAi活性的这种小核酸分子的方法。