公开(公告)号：US20080050721A1

公开(公告)日：2008-02-28

申请号：US10568134

申请日：2004-08-17

申请人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

发明人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6813 ,  Y10T436/143333 ,  C12Q2545/114

摘要： The present invention concerns processes for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridizationdetection assays, antibody-mediated recognition assays, nucleic acid sensor molecules, chromatographic assays, and/or electrophoresis assays. The present invention specifically concerns processes for the detection and quantitation of double stranded nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridization-detection assays. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include molecules that mediate RNA interference, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include nucleic acid aptamers, enzymatic nucleic acid molecules, decoys, antisense, 2,5′-oligoadenylate molecules, triplex forming oligonucleotides or any other nucleic acid molecule of interest. The present invention also concerns kits that allow for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample.

摘要翻译： 本发明涉及使用杂交检测测定，抗体介导的识别测定，核酸传感器分子，色谱测定和/或电泳测定来检测和定量样品中核酸分子，多核苷酸和/或寡核苷酸的方法。 本发明具体涉及使用杂交检测试验检测和定量样品中双链核酸分子，多核苷酸和/或寡核苷酸的方法。 核酸分子，多核苷酸和/或寡核苷酸可以包括介导RNA干扰的分子，例如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA） ，和短发夹RNA（shRNA）分子。 核酸分子，多核苷酸和/或寡核苷酸可以包括核酸适体，酶核酸分子，诱饵，反义，2,5'-寡腺苷酸分子，形成三链体的寡核苷酸或任何其它感兴趣的核酸分子。 本发明还涉及允许在样品中检测和定量核酸分子，多核苷酸和/或寡核苷酸的试剂盒。

公开(公告)号：US07521184B2

公开(公告)日：2009-04-21

申请号：US10568134

申请日：2004-08-17

申请人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

发明人： Susan Radka ,  Zinnen Shawn ,  Vasant Jadhav ,  James McSwiggen ,  Narendra Vaish

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6813 ,  Y10T436/143333 ,  C12Q2545/114

摘要： The present invention concerns processes for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridizationdetection assays, antibody-mediated recognition assays, nucleic acid sensor molecules, chromatographic assays, and/or electrophoresis assays. The present invention specifically concerns processes for the detection and quantitation of double stranded nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample using hybridization-detection assays. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include molecules that mediate RNA interference, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules. The nucleic acid molecules, polynucleotides, and/or oligonucleotides can include nucleic acid aptamers, enzymatic nucleic acid molecules, decoys, antisense, 2′,5′-oligoadenylate molecules, triplex forming oligonucleotides or any other nucleic acid molecule of interest. The present invention also concerns kits that allow for the detection and quantitation of nucleic acid molecules, polynucleotides, and/or oligonucleotides in a sample.

摘要翻译： 本发明涉及使用杂交检测测定，抗体介导的识别测定，核酸传感器分子，色谱测定和/或电泳测定来检测和定量样品中核酸分子，多核苷酸和/或寡核苷酸的方法。 本发明具体涉及使用杂交检测试验检测和定量样品中双链核酸分子，多核苷酸和/或寡核苷酸的方法。 核酸分子，多核苷酸和/或寡核苷酸可以包括介导RNA干扰的分子，例如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA） ，和短发夹RNA（shRNA）分子。 核酸分子，多核苷酸和/或寡核苷酸可以包括核酸适配体，酶核酸分子，诱饵，反义，2'，5'-寡聚腺苷酸分子，形成三链体的寡核苷酸或任何其它感兴趣的核酸分子。 本发明还涉及允许在样品中检测和定量核酸分子，多核苷酸和/或寡核苷酸的试剂盒。