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公开(公告)号:US5679552A
公开(公告)日:1997-10-21
申请号:US450837
申请日:1995-05-25
申请人: Henrik Dalb.o slashed.ge , John Pedersen , Thorkild Christensen , J.o slashed.rli Winnie Ringsted , Torben Ehlern Jessen
发明人: Henrik Dalb.o slashed.ge , John Pedersen , Thorkild Christensen , J.o slashed.rli Winnie Ringsted , Torben Ehlern Jessen
IPC分类号: C07K14/545 , C07K14/61 , C12N9/22 , C12N9/36 , C12N15/62 , C12P21/06 , C12P21/04 , C07H17/00 , C12N15/18
CPC分类号: C12N9/2462 , C07K14/545 , C07K14/61 , C12N15/62 , C12N9/22 , C12P21/06 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75
摘要: A desired protein having the formula: A--B--C--P wherein a) A is Lys or Arg, and B and C are arbitrary amino acids, or b) A is an arbitrary amino acid different from Pro, Lys and Arg, and B and/or C is Pro, is produced from a biosynthetically formed amino acid extended protein having the formula: X--A--B--C--P wherein A, B, C and P are as defined above, and X is an amino acid sequence with an even number of amino acids, of which the first one, seen from the N-terminal end, is different from Lys and Arg, all other uneven amino acids are different from Pro, Lys and Arg, and all even amino acids are different from Pro, by reaction with the enzyme dipeptidyl aminopeptidase (DAP I). The desired protein is obtained in a pure state. Thus, e.g. hGH without content of Met-hGH may be produced by the process.
摘要翻译: 具有下式的所需蛋白质:ABC-P,其中a)A是Lys或Arg,B和C是任意氨基酸,或b)A是不同于Pro,Lys和Arg的任意氨基酸,B和/或C Pro是由具有下式的生物合成形成的氨基酸延伸蛋白产生:XABC-P,其中A,B,C和P如上所定义,X是具有偶数氨基酸的氨基酸序列,其中 第一个,从N末端看,与Lys和Arg不同,所有其他不均匀的氨基酸与Pro,Lys和Arg不同,所有偶联氨基酸与Pro不同,通过与酶二肽基氨基肽酶( DAP I)。 在纯状态下获得所需的蛋白质。 因此,例如 可以通过该过程产生没有Met-hGH含量的hGH。
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公开(公告)号:US5635604A
公开(公告)日:1997-06-03
申请号:US450094
申请日:1995-05-25
申请人: Henrik Dalb.o slashed.ge , John Pedersen , Thorkild Christensen , J.o slashed.rli W. Ringsted , Torben E. Jessen
发明人: Henrik Dalb.o slashed.ge , John Pedersen , Thorkild Christensen , J.o slashed.rli W. Ringsted , Torben E. Jessen
CPC分类号: C12N9/2462 , C07K14/545 , C07K14/61 , C12N15/62 , C12N9/22 , C12P21/06 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75
摘要: A desired protein having the formula:A-B-C-P,wherein (a) A is Lys or Arg, and B and C are arbitrary amino acids, or (b) A is an arbitrary amino acid different from Pro, Lys, and Arg, and B and/or C is Pro is produced from a biosynthetically formed amino-terminally extended protein having the formula:X-A-B-C-P,wherein A, B, C are defined above, and X is an amino acid sequence with an even number of amino acids, of which the first amino acid, seen from the N-terminal end, is different from Lys and Arg, all other am/no acids are different from Pro, Lys and Arg, and all even amino acids are different from Pro, by reaction with the enzyme dipeptidyl aminopeptidase (DAP I). The desired protein is obtained in a pure state. Specifically, P is human growth hormone (hGH). Thus, compositions of hGH may be produced without the presence of Met-hGH.
摘要翻译: 具有下式的所需蛋白质:ABCP,其中(a)A是Lys或Arg,B和C是任意氨基酸,或(b)A是不同于Pro,Lys和Arg的任意氨基酸,B和 /或C是Pro由具有下式的生物合成形成的氨基末端延伸蛋白产生:XABCP,其中A,B,C如上定义,X是具有偶数氨基酸的氨基酸序列,其中 从N-末端观察到的第一个氨基酸不同于Lys和Arg,所有其他am / no酸都不同于Pro,Lys和Arg,并且所有甚至氨基酸与Pro不同,通过与酶二肽基 氨基肽酶(DAP I)。 在纯状态下获得所需的蛋白质。 具体来说,P是人生长激素(hGH)。 因此,可以在不存在Met-hGH的情况下生产hGH的组合物。
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