公开(公告)号：US10584324B2

公开(公告)日：2020-03-10

申请号：US15520567

申请日：2015-10-23

Applicant: NOVOZYMES A/S

Inventor： Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/34 ,  C12P19/14 ,  C12N15/56 ,  C12N1/15 ,  C12P19/02 ,  C12P7/06 ,  C12N9/30 ,  C12N9/44 ,  C12P7/14

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20190256878A1

公开(公告)日：2019-08-22

申请号：US16403919

申请日：2019-05-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  C12N9/34 ,  C12P19/02 ,  C12N9/44 ,  A23L29/30 ,  C12N9/24 ,  C13K1/06 ,  C12N9/26

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US10196620B2

公开(公告)日：2019-02-05

申请号：US15443931

申请日：2017-02-27

Applicant: Novozymes A/S

Inventor： Ming Li ,  Junxin Duan ,  Zheng Liu ,  Shiro Fukuyama ,  Keiichi Ayabe ,  Guillermo Coward-Kelly ,  Randall Deinhammer

IPC: C12N9/34 ,  C12P19/02 ,  C12P19/14 ,  C12P7/06 ,  C13K1/06 ,  C12P7/08

Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20190002855A1

公开(公告)日：2019-01-03

申请号：US16135370

申请日：2018-09-19

Applicant: NOVOZYMES A/S

Inventor： Ming Li ,  Junxin Duan ,  Zheng Liu ,  Shiro Fukuyama ,  Keiichi Ayabe ,  Guillermo Coward-Kelly ,  Randall Deinhammer

IPC: C12N9/34 ,  C12P19/02 ,  C12P7/08 ,  C12P7/06 ,  C12P19/14 ,  C13K1/06

Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20170321237A1

公开(公告)日：2017-11-09

申请号：US15526886

申请日：2015-12-01

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/16 ,  C12P19/02 ,  C12N9/44 ,  C13K1/06 ,  C12N9/34

CPC classification number: C12P19/16 ,  C12N9/2428 ,  C12N9/2457 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01041 ,  C13K1/06

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch, and to compositions useful therein.