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31.发明授权公开(公告)号：US09051588B2
公开(公告)日：2015-06-09
申请号：US12532469
申请日：2008-03-21
IPC分类号： C12N1/20 , C12N9/88 , C12N9/02 , C12N15/00 , C12P7/18 , C07H21/04 , C12N15/70 , C12N9/04 , C12P7/26
CPC分类号： C12P7/18 , C12N9/0004 , C12N9/0006 , C12N9/88 , C12N15/70 , C12P7/26
摘要： The present invention concerns a modified microorganism with an increased methylglyoxal reductase activity, and its use for the preparation of 1,2-propanediol and/or acetol. In particular this increased methylglyoxal reductase activity is obtained by increasing the expression of specific genes from microorganisms.This invention is also related to a method for producing 1,2-propanediol and/or acetol by fermentation of a microorganism having an increased methylglyoxal reductase activity.
摘要翻译： 本发明涉及甲基乙二醛还原酶活性增加的修饰微生物及其用于制备1,2-丙二醇和/或丙酮醇的用途。 特别地，通过增加来自微生物的特异性基因的表达来获得增加的甲基乙二醛还原酶活性。 本发明还涉及通过发酵具有增加的甲基乙二醛还原酶活性的微生物来生产1,2-丙二醇和/或丙酮醇的方法。
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公开(公告)号：US09034611B2
公开(公告)日：2015-05-19
申请号：US13823696
申请日：2011-10-24
申请人： Wanda Dischert , Perrine Vasseur , Cédric Boisart , Rainer Figge
发明人： Wanda Dischert , Perrine Vasseur , Cédric Boisart , Rainer Figge
CPC分类号： C12P13/12 , C12N9/0036 , C12N15/52
摘要： The present invention is related to a microorganism for the production of methionine, wherein said microorganism is modified to enhance the transhydrogenase activity of PntAB. In a preferred aspect of the invention, the activity of the transhydrogenase UdhA is attenuated in said microorganisms. The invention also related to a method for producing methionine by fermentation.
摘要翻译： 本发明涉及用于生产甲硫氨酸的微生物，其中所述微生物被修饰以增强PntAB的转氢酶活性。 在本发明的优选方面，所述微生物中转氢酶UdhA的活性减弱。 本发明还涉及通过发酵生产甲硫氨酸的方法。
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公开(公告)号：US09023624B2
公开(公告)日：2015-05-05
申请号：US13995909
申请日：2010-12-30
申请人： Rainer Figge , Wanda Dischert
发明人： Rainer Figge , Wanda Dischert
摘要： The present invention is related to a method for the fermentative production of hydroxymethionine, comprising the steps of: culturing a recombinant microorganism modified to produce methionine in an appropriate culture medium comprising a source of carbon, a source of sulfur and a source of nitrogen, recovering hydroxymethionine from the culture medium. In a specific embodiment, the recombinant microorganism is cultivated under conditions of nitrogen limitation.The invention is also related to the biologically-produced hydroxymethionine and its uses.
摘要翻译： 本发明涉及羟基甲硫氨酸的发酵生产方法，包括以下步骤：在适当的培养基中培养经修饰以产生甲硫氨酸的重组微生物，所述培养基包含碳源，硫源和氮源，回收 羟基甲硫氨酸。 在一个具体实施方案中，重组微生物在氮限制条件下培养。 本发明还涉及生物学产生的羟基甲硫氨酸及其用途。
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34.发明授权Micro-organisms for the production of 1,2-propanediol obtained by a combination of evolution and rational design 有权用于生产1,2-丙二醇的微生物通过进化和合理设计的组合而获得公开(公告)号：US08298807B2
公开(公告)日：2012-10-30
申请号：US12532405
申请日：2008-03-21
摘要： The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of 1,2-propanediol from a carbon source. The said method comprises growing an initial strain under selection pressure in an appropriate growth medium, said initial bacterial strain comprising an attenuation of the expression of the tpiA gene and an attenuation the expression of at least one gene involved in the conversion of methylglyoxal to lactate, in order to promote evolution in said initial strain; then selecting and isolating the evolved strain having an increased 1,2 propanediol production rate; then reconstructing a functional tpiA gene in the evolved strain. The present invention also concerns the evolved strain such as obtained, that may be furthermore genetically modified in order to optimize the conversion of a carbon source into 1,2-propanediol without bv-products and with the best possible yield.
摘要翻译： 本发明涉及一种组合进化和合理设计的新方法，用于制备用于从碳源生产1,2-丙二醇的微生物菌株。 所述方法包括在选择压力下在合适的生长培养基中生长初始菌株，所述初始细菌菌株包括tpiA基因表达的减弱和减少甲基乙二醛转化为乳酸盐的至少一个基因的表达， 以促进所述初始应变的演化; 然后选择和分离具有增加的1,2-丙二醇生产速率的进化菌株; 然后在演化菌株中重建功能性tpiA基因。 本发明还涉及所获得的进化菌株，其可以进一步进行基因修饰，以便优化碳源转化为1,2-丙二醇而不产生乙腈产物并且以最好的产率。
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35.发明授权Process for chromosomal integration and DNA sequence replacement in Clostridia 有权梭菌染色体整合和DNA序列置换的过程公开(公告)号：US08168434B2
公开(公告)日：2012-05-01
申请号：US11737025
申请日：2007-04-18
CPC分类号： C12N15/74
摘要： The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
摘要翻译： 本发明涉及一种用于在梭菌中替代或缺失DNA序列的新方法，其在工业上具有高效率，易于实施和适用。 该方法可用于以常规方式修饰梭菌中的几个遗传基因座。 该方法基于携带至少两个标记基因的复制载体。
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36.发明申请MICROORGANISMS AND METHODS FOR PRODUCTION OF 1,2-PROPANEDIOL AND ACETOL 有权用于生产1,2-丙二醇和ACETOL的微生物和方法公开(公告)号：US20110201070A1
公开(公告)日：2011-08-18
申请号：US12532469
申请日：2008-03-21
CPC分类号： C12P7/18 , C12N9/0004 , C12N9/0006 , C12N9/88 , C12N15/70 , C12P7/26
摘要： The present invention concerns a modified microorganism with an increased methylglyoxal reductase activity, and its use for the preparation of 1,2-propanediol and/or acetol. In particular this increased methylglyoxal reductase activity is obtained by increasing the expression of specific genes from microorganisms.This invention is also related to a method for producing 1,2-propanediol and/or acetol by fermentation of a microorganism having an increased methylglyoxal reductase activity.
摘要翻译： 本发明涉及甲基乙二醛还原酶活性增加的修饰微生物及其用于制备1,2-丙二醇和/或丙酮醇的用途。 特别地，通过增加来自微生物的特异性基因的表达来获得增加的甲基乙二醛还原酶活性。 本发明还涉及通过发酵具有增加的甲基乙二醛还原酶活性的微生物来生产1,2-丙二醇和/或丙酮醇的方法。
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37.发明申请NEW MICRO-ORGANISMS FOR THE PRODUCTION OF 1,2-PROPANEDIOL OBTAINED BY A COMBINATION OF EVOLUTION AND RATIONAL DESIGN 有权通过组合发展和合理设计获得的1,2-丙二醇的新微生物公开(公告)号：US20100285547A1
公开(公告)日：2010-11-11
申请号：US12532405
申请日：2008-03-21
摘要： The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of 1,2-propanediol from a carbon source.The said method comprises: growing an initial strain under selection pressure in an appropriate growth medium, said initial bacterial strain comprising an attenuation of the expression of the tpiA gene and an attenuation the expression of at least one gene involved in the conversion of methylglyoxal to lactate, in order to promote evolution in said initial strain, then selecting and isolating the evolved strain having an increased 1,2 propanediol production rate, then reconstructing a functional tpiA gene in the evolved strain; The present invention also concerns the evolved strain such as obtained, that may be furthermore genetically modified in order to optimize the conversion of a carbon source into 1,2-propanediol without by-products and with the best possible yield.
摘要翻译： 本发明涉及一种组合进化和合理设计的新方法，用于制备用于从碳源生产1,2-丙二醇的微生物菌株。 所述方法包括：在合适的生长培养基中在选择压力下生长初始菌株，所述初始细菌菌株包括tpiA基因表达的减弱和减少参与将甲基乙二醛转化为乳酸的至少一个基因的表达 为了促进所述初始菌株的进化，然后选择和分离具有增加的1,2-丙二醇生产速率的进化菌株，然后在演化菌株中重建功能性tpiA基因; 本发明还涉及如所获得的进化菌株，其可以进一步经遗传修饰以优化碳源转化为1,2-丙二醇而不产生副产物并以最好的产率。
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38.发明申请METABOLICALLY ENGINEERED MICROORGANISM USEFUL FOR THE PRODUCTION OF 1,2-PROPANEDIOL 审中-公开用于生产1,2-丙二醇的有代表性的工程微生物公开(公告)号：US20100261239A1
公开(公告)日：2010-10-14
申请号：US12532423
申请日：2008-03-21
CPC分类号： C12P7/18 , C12N9/0008
摘要： Microorganism useful for the production of 1,2-propanediol from a carbon source, wherein said microorganism is characterized by: an improved activity of the biosynthesis pathway from dihydroxyacetone phosphate to 1,2-propanediol, and an attenuated activity of the glyceraldehyde 3-phosphate dehydrogenase The invention is also related to a method for producing 1,2-propanediol by fermentation with a microorganism according to the invention.
摘要翻译： 用于从碳源生产1,2-丙二醇的微生物，其中所述微生物的特征在于：从二羟丙基丙酮磷酸酯向1,2-丙二醇的生物合成途径的活性提高，甘油醛-3-磷酸酯的减毒活性 脱氢酶本发明还涉及通过用本发明的微生物发酵生产1,2-丙二醇的方法。
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39.发明申请Process for Chromosomal Integration and DNA Sequence Replacement in Clostridia 有权梭菌染色体整合和DNA序列替代的过程公开(公告)号：US20080085558A1
公开(公告)日：2008-04-10
申请号：US11737025
申请日：2007-04-18
CPC分类号： C12N15/74
摘要： The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
摘要翻译： 本发明涉及一种用于在梭菌中替代或缺失DNA序列的新方法，其在工业上具有高效率，易于实施和适用。 该方法可用于以常规方式修饰梭菌中的几个遗传基因座。 该方法基于携带至少两个标记基因的复制载体。
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