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公开(公告)号:US06268136B1
公开(公告)日:2001-07-31
申请号:US09198083
申请日:1998-11-23
Applicant: Anthony P. Shuber , Stanley N. Lapidus , Gail E. Radcliffe
Inventor: Anthony P. Shuber , Stanley N. Lapidus , Gail E. Radcliffe
IPC: C12Q168
CPC classification number: C12Q1/6886 , C12Q1/6806 , C12Q1/6827 , C12Q2600/156 , C12Q2535/131 , C12Q2565/519 , C12Q2527/125
Abstract: The present invention provides methods for the preparation of stool samples to increase the yield of relevant DNA, and further provides methods for isolating and analyzing target DNA for characteristics indicative of colorectal cancer. Methods for screening patients for the presence of cancerous or pre-cancerous colorectal lesions are also provided.
Abstract translation: 本发明提供了用于制备粪便样品以增加相关DNA产量的方法,并且还提供了用于分离和分析指示结肠直肠癌特征的靶DNA的方法。 还提供了筛选患者癌变或癌前结肠直肠损伤的方法。
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公开(公告)号:US06203993B1
公开(公告)日:2001-03-20
申请号:US09542103
申请日:2000-04-04
Applicant: Anthony P. Shuber , Stanley N. Lapidus , George Q. Daley
Inventor: Anthony P. Shuber , Stanley N. Lapidus , George Q. Daley
IPC: C12Q168
CPC classification number: C12Q1/6816 , C12Q1/6809 , C12Q1/6827 , G06F19/22 , C12Q2535/125
Abstract: Methods are provided for identifying nucleic acids. Methods of the invention are useful for identifying and analyzing nucleic acids, especially variants of single nucleotide polymorphisms, that are indicative of disease or the predisposition for disease.
Abstract translation: 提供了用于鉴定核酸的方法。 本发明的方法可用于鉴定和分析指示疾病或疾病倾向的核酸,特别是单核苷酸多态性的变体。
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公开(公告)号:US6100029A
公开(公告)日:2000-08-08
申请号:US984856
申请日:1997-12-04
Applicant: Stanley N. Lapidus , Anthony P. Shuber
Inventor: Stanley N. Lapidus , Anthony P. Shuber
IPC: G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
CPC classification number: C12Q1/6809 , C12Q1/6816 , C12Q1/6827 , C12Q1/6886 , C12Q2600/156
Abstract: Methods are provided for detecting fetal chromosomal aberrations by detecting statistically-significant differences between normal and aberrant chromosomes.
Abstract translation: 通过检测正常和异常染色体之间的统计学显着性差异来提供检测胎儿染色体畸变的方法。
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公开(公告)号:US5240606A
公开(公告)日:1993-08-31
申请号:US937247
申请日:1992-08-28
Applicant: Stanley N. Lapidus , Lewis T. Polk, Jr. , Fredric L. Farber , J. Morgan Barlas , Anne A. Hurley
Inventor: Stanley N. Lapidus , Lewis T. Polk, Jr. , Fredric L. Farber , J. Morgan Barlas , Anne A. Hurley
CPC classification number: G01N1/2813 , G01N2001/2846
Abstract: An apparatus and method provide automated collection and transfer of particles from a liquid suspension to a glass slide for visual examination. A solution which contains particles, for example cells, is drawn through a filter element so that particles too large to pass through the filter element collect against a first surface of the filter element. A transfer fluid, such as alcohol, is applied from a second surface of the filter element to the first surface, to transfer cells from the filter element to a glass slide positioned adjacent the filter element. An alternative transfer mechanism applies a selected pneumatic signal to the filter element for transferring collected cells to the viewing slide. The apparatus includes a device for dispersing the liquid suspension of particles prior to the collection process and particles collect against the filter element with a spatial distribution advantageous for visual examination. The transfer operation maintains this spatial distribution. The cell-dispersing device and the filter element are on a common structure, for unitary movement, and which is readily disposable and replaceable for each sample of particles.
Abstract translation: 一种装置和方法提供从液体悬浮液到玻璃载玻片的自动收集和转移以进行目视检查。 包含颗粒(例如细胞)的溶液通过过滤元件被吸引,使得太大而不能通过过滤元件的颗粒聚集在过滤元件的第一表面上。 转移流体,例如醇,从过滤元件的第二表面施加到第一表面,以将细胞从过滤元件转移到位于过滤元件附近的玻璃载玻片。 替代的传送机构将所选择的气动信号应用于过滤元件,用于将收集的单元传送到观看幻灯片。 该装置包括用于在收集过程之前分散颗粒的液体悬浮液的装置,并且具有有利于目视检查的空间分布的颗粒聚集在过滤元件上。 转移操作保持这种空间分布。 细胞分散装置和过滤元件在共同的结构上,用于单一运动,并且对于每个颗粒样品而言,其易于一次性和可更换。
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公开(公告)号:US5143627A
公开(公告)日:1992-09-01
申请号:US550142
申请日:1990-07-09
Applicant: Stanley N. Lapidus , Lewis T. Polk, Jr. , Fredric L. Farber , J. Morgan Barlas , Anne A. Hurley
Inventor: Stanley N. Lapidus , Lewis T. Polk, Jr. , Fredric L. Farber , J. Morgan Barlas , Anne A. Hurley
CPC classification number: G01N1/2813 , G01N2001/2846 , Y10T436/25375 , Y10T436/2575
Abstract: An apparatus and method provide automated collection and transfer of particles from a liquid suspension to a glass slide for visual examination. A solution which contains particles, for example cells, is drawn through a filter element so that particles too large to pass through the filter element collect against a first surface of the filter element. A transfer fluid, such as alcohol, is applied from a second surface of the filter element to the first surface, to transfer cells from the filter element to a glass slide positioned adjacent the filter element. An alternative transfer mechanism applies a selected pneumatic signal to the filter element for transferring collected cells to the viewing slide. The apparatus includes a device for dispersing the liquid suspension of particles prior to the collection process and particles collect against the filter element with a spatial distribution advantageous for visual examination. The transfer operation maintains this spatial distribution. The cell-dispersing device and the filter element are on a common structure, for unitary movement, and which is readily disposable and replaceable for each sample of particles.
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