公开(公告)号：US11104937B2

公开(公告)日：2021-08-31

申请号：US15922879

申请日：2018-03-15

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE GENERAL HOSPITAL CORPORATION

发明人： Omar Abudayyeh ,  James Joseph Collins ,  Jonathan Gootenberg ,  Feng Zhang ,  Eric S. Lander ,  Roby Bhattacharyya ,  Deborah Hung

IPC分类号： C12Q1/6832 ,  C12Q1/70 ,  C12Q1/689 ,  C12N15/11 ,  C12N9/22 ,  C12Q1/682 ,  C12Q1/6823 ,  C12N15/10 ,  C12Q1/6895 ,  C12Q1/6893 ,  C12N9/96

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20210130800A1

公开(公告)日：2021-05-06

申请号：US16756139

申请日：2018-10-23

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  David Benjamin Turitz Cox ,  Omar Abudayyeh ,  Ian Slaymaker

IPC分类号： C12N9/22 ,  C12N15/11 ,  A61K9/50 ,  C12N9/78 ,  A61P43/00

摘要： The present disclosure provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the present disclosure provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20210108267A1

公开(公告)日：2021-04-15

申请号：US16955380

申请日：2018-12-20

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Bernd Zetsche ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12Q1/6876 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6804 ,  C12N15/115

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20210093667A1

公开(公告)日：2021-04-01

申请号：US16617560

申请日：2018-06-26

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  David Benjamin Turitz Cox ,  Omar Abudayyeh ,  Soumya Kannan

IPC分类号： A61K35/17 ,  C12N9/78 ,  C12N15/82 ,  C12N15/10 ,  C12N9/22

摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20210009986A1

公开(公告)日：2021-01-14

申请号：US15930478

申请日：2020-05-13

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  David Benjamin Turitz Cox ,  Omar Abudayyeh ,  Soumya Kannan

IPC分类号： C12N15/10 ,  C12N9/22

摘要： The disclosure provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20200332272A1

公开(公告)日：2020-10-22

申请号：US16756134

申请日：2018-10-23

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Mohammad Pirmoradian Najafabadi ,  Qiang Pan-Hammarström ,  Jonathan Gootenberg ,  David Benjamin Turitz Cox ,  Omar Abudayyeh

IPC分类号： C12N9/22 ,  C12N9/78 ,  C12N15/113 ,  C12N15/85 ,  C12N15/10 ,  C12Q1/70 ,  A61K48/00 ,  A61K35/17

摘要： The present disclosure provides methods for inhibiting intra and inter-cellular signaling pathways by modification of post-translation modification sites on select target RNA molecules. In certain example embodiments, the present disclosure provides methods for inhibiting intracellular phosphorylation of serine, threonine and tyrosine residues by editing the genetic codon of these amino acids by means of site-directed RNA editing or RNA molecules. Embodiments disclosed herein further provide methods of inhibiting pathological activation of cell signaling meditated by post-translational modifications, such as phosphorylation, which are involved in many diseases, including cancer, immunodeficiency, infectious diseases, inflammatory disorders and neurodegenerative disorders.

公开(公告)号：US20200291382A1

公开(公告)日：2020-09-17

申请号：US16649170

申请日：2018-09-21

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  David Benjamin Turitz Cox ,  Omar Abudayyeh ,  Soumya Kannan

IPC分类号： C12N15/10 ,  C12N9/22

摘要： The disclosure provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20200283755A1

公开(公告)日：2020-09-10

申请号：US16614549

申请日：2018-05-18

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Jonathan Gootenberg ,  Omar O. Abudayyeh ,  Bernd Zetsche ,  Jonathan Strecker

IPC分类号： C12N15/10 ,  C12N15/62 ,  C12N9/22

摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20200277600A1

公开(公告)日：2020-09-03

申请号：US16645571

申请日：2018-09-07

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12N15/113 ,  C12N9/22 ,  B01L3/00 ,  G01N33/53

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20200254443A1

公开(公告)日：2020-08-13

申请号：US16753896

申请日：2018-10-04

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： B01L3/00 ,  C12Q1/6816 ,  C12Q1/70

摘要： Provided herein is a lateral flow diagnostic device and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The first end may further comprise a first region loaded with a detectable ligand, a CRISPR effector system, a detection construct, a first test band comprising a biotin ligand, and a second test band comprising a capture molecule for the detectable ligand. The detection construct may comprise an RNA oligonucleotide, having a first molecule such as FITC on a first end and a second molecule such as FAM on a second end. Contacting the sample loading portion with a sample causes the sample to flow from the sample loading portion of the substrate towards the first and second capture regions, thereby generating a detectable signal, which may be indicative of a disease state.