公开(公告)号：US11104937B2

公开(公告)日：2021-08-31

申请号：US15922879

申请日：2018-03-15

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE GENERAL HOSPITAL CORPORATION

发明人： Omar Abudayyeh ,  James Joseph Collins ,  Jonathan Gootenberg ,  Feng Zhang ,  Eric S. Lander ,  Roby Bhattacharyya ,  Deborah Hung

IPC分类号： C12Q1/6832 ,  C12Q1/70 ,  C12Q1/689 ,  C12N15/11 ,  C12N9/22 ,  C12Q1/682 ,  C12Q1/6823 ,  C12N15/10 ,  C12Q1/6895 ,  C12Q1/6893 ,  C12N9/96

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20240191223A1

公开(公告)日：2024-06-13

申请号：US18334074

申请日：2023-06-13

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Feng Zhang ,  Omar O. Abudayyeh ,  Jonathan Gootenberg ,  Eric S. Lander

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/82 ,  C12N15/85

CPC分类号： C12N15/102 ,  C12N9/22 ,  C12N15/111 ,  C12N15/113 ,  C12N15/8213 ,  C12N15/8509 ,  C12N2310/20 ,  C12N2800/22

摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

公开(公告)号：US20220154258A1

公开(公告)日：2022-05-19

申请号：US17439063

申请日：2020-03-13

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Jonathan Gootenberg ,  Omar Abudayyeh ,  Feng Zhang

IPC分类号： C12Q1/6825 ,  B01L3/00 ,  C12N9/22 ,  C12N15/11 ,  G16B40/20 ,  G16B20/30 ,  C12N9/14 ,  G01N21/64

摘要： Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences, including multiplex lateral flow diagnostic devices and methods of use, are provided.

公开(公告)号：US20220090178A1

公开(公告)日：2022-03-24

申请号：US17241382

申请日：2021-04-27

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Omar Abudayyeh ,  James Joseph Collins ,  Jonathan Gootenberg ,  Feng Zhang ,  Eric S. Lander

IPC分类号： C12Q1/6832 ,  C12N15/11 ,  C12N9/22 ,  C12N9/96 ,  C12Q1/6816 ,  C12Q1/6823 ,  G01N33/558 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6876 ,  C12Q1/6827 ,  C12Q1/682

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20210207203A1

公开(公告)日：2021-07-08

申请号：US17254886

申请日：2019-06-26

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Max Kellner ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12Q1/6844 ,  C12N15/113 ,  C12N9/22

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide robust CRISPR-based nucleic acid amplification methods and systems. Embodiments disclosed herein can amplify both double-stranded and single-stranded nucleic acid targets. Moreover, the embodiments disclosed herein can be combined with various detection platforms, for example, CRISPR-SHERLOCK, to achieve detection and diagnostic with attomolar sensitivity. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20210009972A1

公开(公告)日：2021-01-14

申请号：US16650480

申请日：2018-10-04

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12N9/22 ,  C12N15/113 ,  C12N9/78 ,  C12N15/86

摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US10266887B2

公开(公告)日：2019-04-23

申请号：US15917549

申请日：2018-03-09

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Omar Abudayyeh ,  James Joseph Collins ,  Jonathan Gootenberg ,  Feng Zhang ,  Eric S. Lander

IPC分类号： C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12N15/113 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6816 ,  C12N9/22 ,  G01N33/00 ,  C12Q1/68

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20240352454A1

公开(公告)日：2024-10-24

申请号：US18430788

申请日：2024-02-02

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12N15/113 ,  B01L3/00 ,  C12N9/22 ,  G01N33/53

CPC分类号： C12N15/113 ,  B01L3/5085 ,  C12N9/22 ,  G01N33/5308 ,  B01L2300/123 ,  B01L2300/126 ,  C12N2310/20 ,  C12N2800/80

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US11685916B2

公开(公告)日：2023-06-27

申请号：US15930478

申请日：2020-05-13

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  David Benjamin Turitz Cox ,  Omar Abudayyeh ,  Soumya Kannan

IPC分类号： C12N15/10 ,  C12N9/22 ,  A61K38/00 ,  C12N9/78 ,  C12N9/12 ,  C07K14/295

CPC分类号： C12N15/102 ,  C12N9/22 ,  A61K38/00 ,  C07K2319/71 ,  C12Y305/04004 ,  C12Y305/04005

摘要： The disclosure provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US11633732B2

公开(公告)日：2023-04-25

申请号：US16753896

申请日：2018-10-04

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12Q1/68 ,  B01L3/00 ,  C12Q1/6816 ,  C12Q1/70

摘要： Provided herein is a lateral flow diagnostic device and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The first end may further comprise a first region loaded with a detectable ligand, a CRISPR effector system, a detection construct, a first test band comprising a biotin ligand, and a second test band comprising a capture molecule for the detectable ligand. The detection construct may comprise an RNA oligonucleotide, having a first molecule such as FITC on a first end and a second molecule such as FAM on a second end. Contacting the sample loading portion with a sample causes the sample to flow from the sample loading portion of the substrate towards the first and second capture regions, thereby generating a detectable signal, which may be indicative of a disease state.