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公开(公告)号：US5158872A
公开(公告)日：1992-10-27
申请号：US565092
申请日：1990-08-10
IPC分类号： C07H15/203 , C12P19/14 , C12P19/18 , C12P19/44 , C12Q1/40
CPC分类号： C07H15/203 , C12P19/14 , C12P19/18 , C12P19/44 , C12Q1/40 , C12Q2334/00 , C12Q2334/10
摘要： An aromatic substituted glycoside is disclosed of the formula ##STR1## wherein the configuration of the substituted --OR on the anomeric carbon is alpha, n is an integer of 0 or 1, and R is a substituted aromatic radical selected from the group ##STR2## where R.sub.1 through R.sub.6 are independently halogen, NO.sub.2, SO.sub.3 H, ##STR3## where R.sub.7 is lower alkyl; and includes its stereoisomers, optical isomers and geometric isomers and mixtures of the foregoing isomers. These substrates are useful as direct substrates for alpha-amylases. A process for the preparation of the substrates and related substances is also described.
摘要翻译： 公开了芳族取代的糖苷，其具有式“IMAGE”，其中在端基异碳上的取代的-OR的构型是α，n是0或1的整数，R是选自下组的取代的芳族基团： （a）其中R 1至R 6独立地为卤素，NO 2，SO 3 H，其中R 7为低级烷基; 并包括其立体异构体，光学异构体和几何异构体以及前述异构体的混合物。 这些底物可用作α-淀粉酶的直接底物。 还描述了制备基底和相关物质的方法。
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32.发明授权公开(公告)号：US08571807B2
公开(公告)日：2013-10-29
申请号：US13308623
申请日：2011-12-01
IPC分类号： G01N33/50
CPC分类号： G06F17/30598 , G06F19/18 , G06F19/24
摘要： The present invention provides methods and systems for an automated method of identifying allele values from data files derived from processed fluorophore emissions detected during the observation of fluorophore labeled nucleotide probes used in analyzing polymorphic DNA are provided. These methods are used in the rapid and efficient distinguishing of targeted polymorphic DNA sites without control samples.
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33.发明申请Computer Algorithm for Automatic Allele Determination from Fluorometer Genotyping Device 有权计算机算法用于自动等位基因测定从荧光基因分型装置公开(公告)号：US20120143872A1
公开(公告)日：2012-06-07
申请号：US13308623
申请日：2011-12-01
IPC分类号： G06F17/30
CPC分类号： G06F17/30598 , G06F19/18 , G06F19/24
摘要： The present invention provides methods and systems for an automated method of identifying allele values from data files derived from processed fluorophore emissions detected during the observation of fluorophore labeled nucleotide probes used in analyzing polymorphic DNA are provided. These methods are used in the rapid and efficient distinguishing of targeted polymorphic DNA sites without control samples.
摘要翻译： 本发明提供了用于从用于分析多态性DNA的荧光团标记的核苷酸探针观察期间检测到的经处理的荧光团发射的数据文件中鉴定等位基因值的自动化方法的方法和系统。 这些方法用于快速高效地区分不含对照样品的靶向多态性DNA位点。
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34.发明授权Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon 失效具有分子能量转移标记的核酸扩增寡核苷酸和基于其的方法
公开(公告)号：US6090552A
公开(公告)日：2000-07-18
申请号：US891516
申请日：1997-07-11
CPC分类号： C12Q1/6818 , C12Q1/6844 , C12Q1/6858 , C12Q1/686
摘要： The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
摘要翻译： 本发明提供标记的核酸扩增寡核苷酸，其可以是线性或发夹引物或阻断寡核苷酸。 本发明的寡核苷酸用分子能量转移对的供体和/或受体部分标记。 这些部分可以是荧光团，使得供体发射的荧光能被受体吸收。 受体可以是在与供体部分不同的波长处发荧光的荧光团，或者它可以是猝灭剂。 配置本发明的寡核苷酸使得供体部分和受体部分被并入扩增产物。 本发明还提供了使用核酸扩增引物直接检测扩增产物的方法和试剂盒。 当使用标记的线性引物时，用外切核酸酶处理或通过使用特定温度消除了对未引入引物的分离的需要。 这种“封闭管”格式大大降低了放大产品携带污染的可能性，提供了高通量的样品，并且可以完全自动化。
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35.发明授权
公开(公告)号：US5989871A
公开(公告)日：1999-11-23
申请号：US800641
申请日：1997-02-14
IPC分类号： C12N15/09 , B01D57/02 , C12Q1/68 , G01N27/447 , G01N30/88 , G01N33/566 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6818 , Y10T436/143333
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieiving medium.
摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳进行分级，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。
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公开(公告)号：US5320954A
公开(公告)日：1994-06-14
申请号：US937255
申请日：1992-09-03
CPC分类号： C07H15/203 , C12P19/14 , C12P19/18 , C12P19/44 , C12Q1/40 , C12Q2334/00 , C12Q2334/10
摘要： An aromatic substituted glycoside is disclosed of the formula ##STR1## wherein the configuration of the substituted --OR on the anomeric carbon is alpha, n is an integer of 0 or 1, and R is a substituted aromatic radical selected from the group ##STR2## where R.sub.1 through R.sub.6 are independently halogen, NO.sub.2, SO.sub.3 H, COR.sub.7, ##STR3## where R.sub.7 is lower alkyl; and includes its stereoisomers, optical isomers and geometric isomers and mixtures of the foregoing isomers. These substrates are useful as direct substrates for alpha-amylases. A process for the preparation of the substrates and related substances is also described.
摘要翻译： 公开了芳族取代的糖苷，其具有式“IMAGE”，其中在端基异碳上的取代的-OR的构型是α，n是0或1的整数，R是选自下组的取代的芳族基团： （a）其中R 1至R 6独立地为卤素，NO 2，SO 3 H，COR 7，其中R 7为低级烷基;（b）和（c） 并包括其立体异构体，光学异构体和几何异构体以及前述异构体的混合物。 这些底物可用作α-淀粉酶的直接底物。 还描述了制备基底和相关物质的方法。
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37.发明授权Reagent system for an alpha-amylase assay containing aromatic substituted glycoside 失效含有芳香取代糖苷的α-淀粉酶测定试剂系统
公开(公告)号：US4963479A
公开(公告)日：1990-10-16
申请号：US091861
申请日：1987-09-04
IPC分类号： C12P19/14 , C07H15/203 , C12P19/18 , C12P19/44 , C12Q1/40
CPC分类号： C07H15/203 , C12P19/14 , C12P19/18 , C12P19/44 , C12Q1/40 , C12Q2334/00 , C12Q2334/10
摘要： An aromatic substituted glycoside is disclosed of the formula ##STR1## wherein the configuration of the substituted --OR on the anomeric carbon is alpha, n is an integer of 0 to 1, and R is a substituted aromatic radical selected from the group ##STR2## where R.sub.1 through R.sub.6 are independently halogen, NO.sub.2, SO.sub.3 H, ##STR3## where R.sub.7 is lower alkyl; and includes its stereoisomers, optical isomers and geometric isomers and mixtures of the foregoing isomers. These substrates are useful as direct substrates for alpha-amylases. A process for the preparation of the substrates and related substances is also described.
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