公开(公告)号：US20050221510A1

公开(公告)日：2005-10-06

申请号：US11134749

申请日：2005-05-19

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N21/65 ,  G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

公开(公告)号：US20050221509A1

公开(公告)日：2005-10-06

申请号：US11134747

申请日：2005-05-19

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N21/65 ,  G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

摘要翻译： 本发明基于这样的发现：本文所述的用于生产金属胶体的方法导致胶体表现出增加的信号增强和生物分子的SERS检测的再现性。 因此，使用本发明的方法，可以以更高的灵敏度和可靠性检测各种各样的生物分子。

公开(公告)号：US20050142567A1

公开(公告)日：2005-06-30

申请号：US10830422

申请日：2004-04-21

申请人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

发明人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

IPC分类号： G01N21/65 ,  G01N33/543 ,  C12Q1/68 ,  G01N33/553

CPC分类号： G01N33/54346 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/658 ,  G01N2021/6482 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

摘要： Composite organic-inorganic nanoparticles (COIN) and clusters of such nanoparticles are provided that produce surface-enhanced Raman signals when excited by a laser. The nanoparticles include metallic colloids and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanoparticle, and a wide variety of Raman-active organic compounds can be incorporated into the particle. Methods for producing the nanoparticles and clusters of nanoparticles are also provided. In addition, polymeric microspheres containing the nanoparticles and clusters of nanoparticles and methods of making them are also provided. Methods for using the nanoparticles, clusters, and microspheres in assays for multiplex detection of biological molecules do not require signal amplification techniques.

摘要翻译： 提供复合有机 - 无机纳米颗粒（COIN）和这种纳米颗粒的簇，当激光激发时产生表面增强的拉曼信号。 纳米颗粒包括金属胶体和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米颗粒中是固有的，并且可以将多种拉曼活性有机化合物掺入颗粒中。 还提供了制备纳米颗粒和纳米颗粒簇的方法。 此外，还提供了包含纳米颗粒和纳米颗粒簇的聚合物微球及其制备方法。 在用于多重检测生物分子的测定中使用纳米颗粒，簇和微球的方法不需要信号放大技术。