公开(公告)号：US20070196233A1

公开(公告)日：2007-08-23

申请号：US11709026

申请日：2007-02-22

申请人： Taisei Nishimi ,  Toshihide Ezoe

发明人： Taisei Nishimi ,  Toshihide Ezoe

IPC分类号： G01N33/00

CPC分类号： G01N33/553 ,  Y10T436/201666

摘要： An object of the present invention is to provide a biosensor and a method for immobilizing a physiologically active substance, by which preconcentration effects can be obtained at a pH that is equivalent to or higher than the isoelectric point of the physiologically active substance and the physiologically active substance can be covalently bound to the surface. The present invention provides a biosensor comprising a solid substrate to which a polymer having a primary or secondary amino group is bound, by which a physiologically active substance can be chemically immobilized following preconcentration of the substance at a pH that is equivalent to or higher than the isoelectric point of the substance.

摘要翻译： 本发明的目的是提供一种生物传感器和固定生物活性物质的方法，通过该生物传感器和生物活性物质的生理活性物质的等电点的pH值可以获得预浓缩效果， 物质可以共价结合到表面。 本发明提供了一种生物传感器，其包含具有伯或仲氨基的聚合物所固定的固体基质，通过该生物传感器，物理活性物质可以在相当于或高于 物质的等电点。

公开(公告)号：US20070071642A1

公开(公告)日：2007-03-29

申请号：US11524303

申请日：2006-09-21

申请人： Taisei Nishimi ,  Toshihide Ezoe

发明人： Taisei Nishimi ,  Toshihide Ezoe

IPC分类号： G01N21/00

CPC分类号： G01N33/54393 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/553

摘要： It is an object of the present invention to provide a technique of conserving a functional group used for binding a physiologically active substance in the form of an inactive ester, and converting it to an activated ester by simple operations immediately before formation of an amide bond. The present invention provides a biosensor which comprises a substrate that has been modified with a phenyl ester group having a substituent whose electron-withdrawing ability is increased as a result of a chemical reaction.

摘要翻译： 本发明的目的是提供一种保护用于结合无活性酯形式的生理活性物质的官能团的技术，并且在形成酰胺键之前通过简单的操作将其转化为活化的酯。 本发明提供了一种生物传感器，其包括由具有吸电子性能的化合物反应性增加的具有取代基的苯基酯基改性的基板。