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公开(公告)号：US08585972B2
公开(公告)日：2013-11-19
申请号：US12778555
申请日：2010-05-12
IPC分类号： G01N33/53
CPC分类号： G01N33/54353 , G01N33/54373
摘要： One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.
摘要翻译： 本发明的一个目的是提供一种生物传感器及其生产方法，通过该生物传感器及其生产方法，可以使用安全的原料方便地生产能够固定生理活性物质的水凝胶。 本发明提供一种生物传感器，其包括在其表面具有金属层的基材，其中具有能够与生理活性物质的羟基或氨基反应的反应性官能团的亲水性聚合物直接结合到金属层 或间接经由中间层。
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公开(公告)号：US20100221845A1
公开(公告)日：2010-09-02
申请号：US12778555
申请日：2010-05-12
IPC分类号： G01N33/53
CPC分类号： G01N33/54353 , G01N33/54373
摘要： One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.
摘要翻译： 本发明的一个目的是提供一种生物传感器及其生产方法，通过该生物传感器及其生产方法，可以使用安全的原料方便地生产能够固定生理活性物质的水凝胶。 本发明提供一种生物传感器，其包括在其表面具有金属层的基材，其中具有能够与生理活性物质的羟基或氨基反应的反应性官能团的亲水性聚合物直接结合到金属层 或间接经由中间层。
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公开(公告)号：US20080188010A1
公开(公告)日：2008-08-07
申请号：US12022606
申请日：2008-01-30
申请人： Yukou Saitoh , Toshihide Ezoe , Taisei Nishimi
发明人： Yukou Saitoh , Toshihide Ezoe , Taisei Nishimi
IPC分类号： G01N33/551 , B01J19/00
CPC分类号： G01N33/54373
摘要： It is an object of the present invention to provide a biosensor substrate that prevents non-specific adsorption and is used for immobilizing a physiologically active substance. The present invention provides a biosensor substrate which comprises an inorganic oxide film and a hydrophilic polymer having a physiologically active substance-immobilizing group on the substrate, wherein the hydrophilic polymer is bound to the inorganic oxide film through a low molecular compound.
摘要翻译： 本发明的目的是提供一种防止非特异性吸附并用于固定生理活性物质的生物传感器基板。 本发明提供一种生物传感器基板，其包含无机氧化物膜和在基板上具有生理活性物质固定基团的亲水性聚合物，其中亲水性聚合物通过低分子化合物与无机氧化物膜结合。
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公开(公告)号：US20080090306A1
公开(公告)日：2008-04-17
申请号：US11862682
申请日：2007-09-27
申请人： Koji KURUMA , Toshihide Ezoe
发明人： Koji KURUMA , Toshihide Ezoe
IPC分类号： G01N33/543 , G01N21/55
CPC分类号： G01N33/54353 , G01N21/553 , G01N33/54373 , G01N33/553
摘要： An object of the present invention is to provide a method for preventing the generation of negative signals because of dissociation of a ligand from a surface and improving the precision of signals indicating binding between a ligand and an analyte. The present invention provides a method for immobilizing a biomolecule on a carrier having reactive groups which comprises: (i) a step of activating some reactive groups so that they can form a covalent bond with a biomolecule; (ii) a step of reacting the biomolecule with the aforementioned activated reactive groups; and (iii) a step of again activating some reactive groups; wherein the steps (i), (ii) and (iii) are performed in this order.
摘要翻译： 本发明的目的是提供一种防止由于配体与表面解离而产生负信号的方法，提高了表示配体与分析物之间结合的信号的精度。 本发明提供了将生物分子固定在具有反应性基团的载体上的方法，该方法包括：（i）使一些反应性基团活化以使其与生物分子形成共价键的步骤; （ii）使生物分子与上述活化的反应性基团反应的步骤; 和（iii）再次激活一些反应性基团的步骤; 其中步骤（i），（ii）和（iii）按此顺序执行。
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公开(公告)号：US20060246512A1
公开(公告)日：2006-11-02
申请号：US11377554
申请日：2006-03-17
申请人： Toshihide Ezoe , Taisei Nishimi , Yukou Saito
发明人： Toshihide Ezoe , Taisei Nishimi , Yukou Saito
CPC分类号： C12Q1/002 , G01N21/553 , G01N21/554 , G01N33/5438 , Y10S436/805
摘要： It is an object of the present invention to provide a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The present invention provides a biosensor which comprises a substrate having at least two types of surfaces on a single plane, wherein at least one of said surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to said surface that does not retain a physiologically active substance.
摘要翻译： 本发明的目的是提供一种生物传感器，其中抑制了不保留生理活性物质（参考单元）的表面上的非特异性吸附。 本发明提供了一种生物传感器，其包括在单个平面上具有至少两种类型的表面的基底，其中所述表面中的至少一个不保留生理活性物质，并且其中至少两种具有不同分子量的亲水性化合物 结合到不保留生理活性物质的所述表面。
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公开(公告)号：US20060211137A1
公开(公告)日：2006-09-21
申请号：US11362354
申请日：2006-02-27
申请人： Toshihide Ezoe , Toshiaki Kubo , Masashi Hakamata
发明人： Toshihide Ezoe , Toshiaki Kubo , Masashi Hakamata
IPC分类号： G01N33/543 , C12M1/34
CPC分类号： G01N33/54373
摘要： It is an object of the present invention to provide a biosensor wherein a reference unit and a measurement unit can be prepared by performing only a single operation for immobilizing a physiologically active substance and wherein unnecessary electric charge does not remain in the reference unit. The present invention provides a biosensor which comprises a substrate composed of a metal surface or metal film coated with a hydrophilic polymer compound, and which has a surface for retaining a physiologically active substance and a surface that does not retain a physiologically active substance on a single plane of the substrate.
摘要翻译： 本发明的目的是提供一种生物传感器，其中通过仅执行用于固定生理活性物质的单一操作并且其中不必要的电荷不残留在参考单元中，可以制备参考单元和测量单元。 本发明提供了一种生物传感器，其包括由涂覆有亲水性高分子化合物的金属表面或金属膜构成的基板，并且具有用于保持生理活性物质的表面和不保留生理活性物质的表面 基板的平面。
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公开(公告)号：US20060040410A1
公开(公告)日：2006-02-23
申请号：US11205183
申请日：2005-08-17
申请人： Taisei Nishimi , Toshihide Ezoe , Toshiaki Kubo
发明人： Taisei Nishimi , Toshihide Ezoe , Toshiaki Kubo
IPC分类号： C12M1/34 , G01N33/553
CPC分类号： G01N33/54393 , G01N33/54373
摘要： It is an object of the present invention to provide a technique of converting carboxylic acid into an active ester with no generation of air bubbles and a technique of stabilizing the obtained active ester. The present invention provides a biosensor wherein a carboxyl group existing on the surface of a substrate thereof is activated with any one compound selected from an uronium salt, a phosphonium salt or a triazine derivative which are defined in the present application, so as to form a carboxylic acid amide group.
摘要翻译： 本发明的目的是提供一种不产生气泡而将羧酸转化成活性酯的技术，以及稳定所得活性酯的技术。 本发明提供一种生物传感器，其中存在于其底物表面上的羧基由选自本申请中定义的铀盐，鏻盐或三嗪衍生物的任何一种化合物活化，从而形成 羧酸酰胺基。
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公开(公告)号：US20060003395A1
公开(公告)日：2006-01-05
申请号：US11137390
申请日：2005-05-26
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/537 , G01N33/543 , C12M1/34
CPC分类号： G01N33/54373 , Y10S435/808 , Y10S436/805 , Y10S436/806
摘要： It is an object of the present invention to provide a method for simultaneously carrying out the detection or measurement of a substance interacting with a physiologically active substance immobilized on a biosensor and the analysis of the effects of the above substance on the biological activity of the physiologically active substance. The present invention provides a measurement method using a biosensor formed by immobilizing a physiologically active substance on a substrate, wherein the detection and measurement of a substance interacting with said physiologically active substance and the measurement of the biological activity of said physiologically active substance are carried out on said single biosensor.
摘要翻译： 本发明的目的是提供一种同时进行与固定在生物传感器上的生理活性物质相互作用的物质的检测或测量的方法，以及上述物质对生理活性的生物学活性的影响的分析 活性物质。 本发明提供一种使用通过将生理活性物质固定在基材上形成的生物传感器的测量方法，其中进行与所述生理活性物质相互作用的物质的检测和测量以及所述生理活性物质的生物活性的测量。 在所述单个生物传感器上。
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9.发明授权Silver halide photographic material and photographic image formation method using the same 失效卤化银照相材料和使用其的摄影图像形成方法
公开(公告)号：US5496681A
公开(公告)日：1996-03-05
申请号：US393170
申请日：1995-02-21
申请人： Toshihide Ezoe , Tomokazu Yasuda
发明人： Toshihide Ezoe , Tomokazu Yasuda
摘要： A silver halide photographic material is described, which comprises a hydrazine derivative represented by the following formula (I) and a surface active compound represented by the following formula (II): ##STR1## and wherein the substituents are defined in the specification. An image formation method using the silver halide photographic material is also described.
摘要翻译： 描述了卤化银照相材料，其包含由下式（I）表示的肼衍生物和由下式（II）表示的表面活性化合物：其中取代基在说明书中定义 。 还描述了使用卤化银照相材料的成像方法。
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公开(公告)号：US07674633B2
公开(公告)日：2010-03-09
申请号：US11524303
申请日：2006-09-21
申请人： Taisei Nishimi , Toshihide Ezoe
发明人： Taisei Nishimi , Toshihide Ezoe
IPC分类号： G01N33/553
CPC分类号： G01N33/54393 , B82Y15/00 , B82Y30/00 , G01N21/553
摘要： It is an object of the present invention to provide a technique of conserving a functional group used for binding a physiologically active substance in the form of an inactive ester, and converting it to an activated ester by simple operations immediately before formation of an amide bond. The present invention provides a biosensor which comprises a substrate that has been modified with a phenyl ester group having a substituent whose electron-withdrawing ability is increased as a result of a chemical reaction.
摘要翻译： 本发明的目的是提供一种保护用于结合无活性酯形式的生理活性物质的官能团的技术，并且在形成酰胺键之前通过简单的操作将其转化为活化的酯。 本发明提供了一种生物传感器，其包括由具有吸电子性能的化合物反应性增加的具有取代基的苯基酯基改性的基板。
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