公开(公告)号：US20190382828A1

公开(公告)日：2019-12-19

申请号：US16007347

申请日：2018-06-13

Applicant: International Business Machines Corporation

Inventor： Stacey Gifford ,  Sung-Cheol Kim ,  Joshua T. Smith ,  Benjamin Wunsch

IPC: C12Q1/6813 ,  C12Q1/6825 ,  C12Q1/6806

Abstract: Techniques regarding detecting one or more defined nucleic acid sequences are provided. For example, one or more embodiments described herein can comprise a method, which can comprise adding a molecular probe to a sample fluid comprising a first deoxyribonucleic acid segment and a second deoxyribonucleic acid segment. The molecular probe can have an affinity to bond to a defined nucleic acid sequence. The method can also comprise separating, via a nanoscale deterministic lateral displacement array, the first deoxyribonucleic acid segment from the second deoxyribonucleic acid segment based on a size of the first deoxyribonucleic acid segment.

公开(公告)号：US20190382821A1

公开(公告)日：2019-12-19

申请号：US16007389

申请日：2018-06-13

Applicant: International Business Machines Corporation

Inventor： Stacey Gifford ,  Benjamin Wunsch ,  Joshua T. Smith ,  Sung-Cheol Kim

IPC: C12Q1/683 ,  C12Q1/6816

Abstract: Techniques regarding screening for mutations using nanoscale deterministic arrays are provided. For example, one or more embodiments described herein can comprise a method, which can comprise cleaving a deoxyribonucleic acid segment hybridized with a molecular probe to form a sample fluid. The cleaving can occur at a first end and a second end of the molecular probe. Also, the cleaving can comprise a cleaving agent that targets base pair mismatches. The method can also comprise supplying the sample fluid to a nanoscale deterministic lateral displacement array to screen for a single nucleotide polymorphism.

公开(公告)号：US20190300932A1

公开(公告)日：2019-10-03

申请号：US15942726

申请日：2018-04-02

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Stacey Gifford ,  Benjamin Wunsch

IPC: C12Q1/6806

Abstract: A method of purifying nucleic acid polymers for next generation sequencing includes loading a mixture of adapter ligated, fragmented nucleic acid polymers onto a nano-deterministic lateral displacement (nanoDLD) array. The mixture includes a first adapter ligated, fragmented nucleic acid polymer, a second adapter ligated, fragmented nucleic acid polymer, and a contaminant. The method further includes flowing the mixture through the nanoDLD array to separate the first adapter ligated, fragmented nucleic acid polymer from the second adapter ligated, fragmented nucleic acid polymer, and each of the first and second adapter ligated, fragmented nucleic acid polymers from the contaminant.