公开(公告)号：US20160152960A1

公开(公告)日：2016-06-02

申请号：US14787418

申请日：2014-04-29

Applicant: NOVOZYMES A/S

Inventor： Noriko Tsutsumi ,  Keiichi Ayabe ,  Siik Kishishita

IPC: C12N9/34 ,  C12P19/20 ,  C12P19/02 ,  A23L1/23

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及葡糖淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US20150210995A1

公开(公告)日：2015-07-30

申请号：US14420399

申请日：2013-08-15

Applicant: NOVOZYMES A/S

Inventor： Tomoko Matsui ,  Aki Tomiki ,  Allan Svendsen ,  Hanne Vang Hendriksen ,  Mary Ann Stringer ,  Keiichi Ayabe

IPC: C12N9/82

CPC classification number: C12N9/82 ,  A23L5/25 ,  C12Y305/01001

Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

Abstract translation: 本发明涉及天冬酰胺酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。 本发明进一步涉及一种生产发酵产物的方法，包括：在本发明的天冬酰胺酶存在下，将含淀粉的物质用α-淀粉酶液化; 将糊精糖化成糖与葡糖淀粉酶; 并使用发酵生物发酵糖。

公开(公告)号：US11447763B2

公开(公告)日：2022-09-20

申请号：US17148790

申请日：2021-01-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US11377648B2

公开(公告)日：2022-07-05

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12N15/52 ,  C12N15/62 ,  C12N15/82 ,  C12N1/16

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US20210139876A1

公开(公告)日：2021-05-13

申请号：US17148790

申请日：2021-01-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20210139875A1

公开(公告)日：2021-05-13

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P19/14 ,  C12P19/02 ,  C12N15/82 ,  C12P7/06

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US10584324B2

公开(公告)日：2020-03-10

申请号：US15520567

申请日：2015-10-23

Applicant: NOVOZYMES A/S

Inventor： Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/34 ,  C12P19/14 ,  C12N15/56 ,  C12N1/15 ,  C12P19/02 ,  C12P7/06 ,  C12N9/30 ,  C12N9/44 ,  C12P7/14

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10550374B2

公开(公告)日：2020-02-04

申请号：US14787418

申请日：2014-04-29

Applicant: NOVOZYMES A/S

Inventor： Noriko Tsutsumi ,  Keiichi Ayabe ,  Siik Kishishita

IPC: C12N9/34 ,  C12P19/20 ,  C12P19/02

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20190352629A1

公开(公告)日：2019-11-21

申请号：US16318496

申请日：2017-07-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben Peter Friis ,  Jens Erik Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20190256878A1

公开(公告)日：2019-08-22

申请号：US16403919

申请日：2019-05-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  C12N9/34 ,  C12P19/02 ,  C12N9/44 ,  A23L29/30 ,  C12N9/24 ,  C13K1/06 ,  C12N9/26

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.