公开(公告)号：US20220154226A1

公开(公告)日：2022-05-19

申请号：US17440477

申请日：2020-03-17

Applicant: Novozymes A/S

Inventor： Takashi Nakanishi ,  Aki Tomiki ,  Yuma Kurakata

IPC: C12P7/06

Abstract: The present invention relates to a variant pullulanase, having increased thermo-stability and/or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399, 401, 402, 411, 412, 434, 435, 443, 446, 459, 460, 479, 490, 498, 514, 529, 531, 533, 541, 545, 581, 583, 595, 649, 665, 688, 700, 709, 804, 811 of SEQ ID NO: 1, and optionally a deletion of one or more, e.g., all amino acids at positions 821, 822, 823, 824, 825, 826, 827, and 828, wherein the variant has pullulanase activity, and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.

公开(公告)号：US11377648B2

公开(公告)日：2022-07-05

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12N15/52 ,  C12N15/62 ,  C12N15/82 ,  C12N1/16

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US20210139875A1

公开(公告)日：2021-05-13

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P19/14 ,  C12P19/02 ,  C12N15/82 ,  C12P7/06

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US20230407273A1

公开(公告)日：2023-12-21

申请号：US18249037

申请日：2021-10-08

Applicant: Novozymes A/S

Inventor： Michiko Ihara ,  Takashi Nakanishi

IPC: C12N9/10 ,  C12N15/80 ,  C12N9/34 ,  C12N9/36

CPC classification number: C12N9/1048 ,  C12N15/80 ,  C12N9/2428 ,  C12N9/2462 ,  C12R2001/66

Abstract: The present invention relates to a polynucleotide variant encoding a glycosyltransferase variant, and to nucleic acid constructs, vectors and host cells comprising said polynucleotide variant. The invention also relates to methods of producing a polypeptide of interest in host cells comprising said polynucleotide and/or glycosyltransferase variant.