公开(公告)号：US07405285B2

公开(公告)日：2008-07-29

申请号：US11234396

申请日：2005-09-22

申请人： Sudhir Agrawal ,  Ekambar Kandimalla ,  Dong Yu

发明人： Sudhir Agrawal ,  Ekambar Kandimalla ,  Dong Yu

IPC分类号： C07H21/02

CPC分类号： A61K31/711 ,  A61K38/2013 ,  A61K39/39 ,  A61K2039/55561 ,  A61N2005/1098 ,  C12N15/117 ,  C12N2310/17 ,  C12N2310/315 ,  C12N2310/318 ,  C12N2310/334 ,  C12N2310/336 ,  C12N2310/3519 ,  A61K2300/00

摘要： The invention provides an immunostimulatory nucleic acid. In certain embodiments according to this aspect of the invention, the sequence of the immunostimulatory oligonucleotide and/or immunomer is at least partially self-complementary.

摘要翻译： 本发明提供免疫刺激性核酸。 在根据本发明的该方面的某些实施方案中，免疫刺激性寡核苷酸和/或免疫反应物的序列至少部分自身互补。

公开(公告)号：US07276489B2

公开(公告)日：2007-10-02

申请号：US10279684

申请日：2002-10-24

申请人： Sudhir Agrawal ,  Ekambar R. Kandimalla ,  Dong Yu ,  Lakshmi Bhagat

发明人： Sudhir Agrawal ,  Ekambar R. Kandimalla ,  Dong Yu ,  Lakshmi Bhagat

IPC分类号： C07H21/02 ,  C07H21/04 ,  A01N43/04 ,  A61K31/70

CPC分类号： C07H21/04 ,  A61K39/39 ,  A61K2039/55561

摘要： The invention relates to the therapeutic use of oligonucleotides as immunostimulatory agents in immunotherapy applications. More particularly, the invention provides immunomers for use in methods for generating an immune response or for treating a patient in need of immunostimulation. The immunomers of the invention comprise at least two oligonucleotides linked at their 3′ ends, internucleoside linkages or functionalized nucleobase or sugar to a non-nucleotidic linker, at least one of the oligonucleotides being an immunostimulatory oligonucleotide and having an accessible 5′ end.

公开(公告)号：US07262286B2

公开(公告)日：2007-08-28

申请号：US09965116

申请日：2001-09-26

申请人： Ekambar R. Kandimalla ,  Qiuyan Zhao ,  Dong Yu ,  Sudhir Agrawal

发明人： Ekambar R. Kandimalla ,  Qiuyan Zhao ,  Dong Yu ,  Sudhir Agrawal

IPC分类号： C07H21/00

CPC分类号： C12N15/117 ,  A61K31/7088 ,  A61K31/7115 ,  A61K2039/55561 ,  C07H21/00 ,  C12N2310/18 ,  C12N2310/3125 ,  C12N2310/317 ,  C12N2310/318 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/33 ,  C12N2310/334 ,  C12N2310/336 ,  C12N2310/3521

摘要： The invention relates to the therapeutic use of oligonucleotides or oligonucleotide analogs as immunostimulatory agents in immunotherapy applications. The invention provides methods for enhancing the immune response caused by immunostimulatory oligonucleotide compounds.

公开(公告)号：US08946175B1

公开(公告)日：2015-02-03

申请号：US13210903

申请日：2011-08-16

申请人： Ekambar R. Kandimalla ,  Lakshmi Bhagat ,  Rajendra K. Pandey ,  Dong Yu ,  Sudhir Agrawal

发明人： Ekambar R. Kandimalla ,  Lakshmi Bhagat ,  Rajendra K. Pandey ,  Dong Yu ,  Sudhir Agrawal

IPC分类号： A61K48/00 ,  A61K45/00 ,  A61K47/00 ,  A61K39/00 ,  A61K39/38

CPC分类号： C12N15/117 ,  C12N2310/17 ,  C12N2310/315 ,  C12N2310/3183 ,  C12N2310/321 ,  C12N2310/336 ,  C12N2310/53 ,  C12N2320/51 ,  C12N2310/3521

摘要： The invention provides immunostimulatory oligonucleotides having at least one CpG dinucleotide and a secondary structure at the 5′- or 3′-end. These oligonucleotides have either reduced or improved immunostimulatory properties. The invention establishes that 5′-terminal secondary structures affect immunostimulatory activity significantly more than those at the 3′-end. The invention also provides methods for increasing or decreasing the immunostimulatory activity of a CpG-containing nucleic acid.

摘要翻译： 本发明提供了在5'-或3'-末端具有至少一个CpG二核苷酸和二级结构的免疫刺激性寡核苷酸。 这些寡核苷酸具有降低的或改善的免疫刺激性质。 本发明确定5'-末端二级结构比3'-末端显着地影响免疫刺激活性。 本发明还提供了增加或降低含CpG的核酸的免疫刺激活性的方法。

公开(公告)号：US20120201807A1

公开(公告)日：2012-08-09

申请号：US13404309

申请日：2012-02-24

申请人： Sudhir Agrawal ,  Lakshmi Bhagat ,  Dong Yu ,  Ekambar R. Kandimalla

发明人： Sudhir Agrawal ,  Lakshmi Bhagat ,  Dong Yu ,  Ekambar R. Kandimalla

IPC分类号： A61K39/00 ,  A61P37/04 ,  A61K39/35 ,  C07H21/04 ,  A61K39/395

CPC分类号： C12N15/117 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/111 ,  C12N2310/17 ,  C12N2310/33 ,  C12N2310/3519 ,  C12N2310/51

摘要： The invention relates to the therapeutic use of oligonucleotides as immunostimulatory agents in immunotherapy applications. More particularly, the invention provides immunomers and immunostimulatory oligonucleotides for use in methods for generating an immune response or for treating a patient in need of immunostimulation. The immunomers and immunostimulatory oligonucleotides of the invention preferably comprise novel purines. The immunomers according to the invention further comprise at least two oligonucleotides linked at their 3′ ends, internucleoside linkages or functionalized nucleobase or sugar to a non-nucleotidic linker, at least one of the oligonucleotides being an immunomodulatory oligonucleotide and having an accessible 5′ end.

摘要翻译： 本发明涉及在免疫治疗应用中寡核苷酸作为免疫刺激剂的治疗用途。 更具体地，本发明提供用于产生免疫应答的方法或用于治疗需要免疫刺激的患者的免疫和免疫刺激性寡核苷酸。 本发明的免疫和免疫刺激寡核苷酸优选包含新的嘌呤。 根据本发明的免疫反应物还包含至少两个在其3'末端连接的寡核苷酸，核苷间键或官能化核碱基或糖与非核酸连接体，至少一个寡核苷酸是免疫调节性寡核苷酸并具有可接近的5'末端 。

公开(公告)号：US20120107303A1

公开(公告)日：2012-05-03

申请号：US13006860

申请日：2011-01-14

申请人： Ekambar R. Kandimalla ,  Sudhir Agrawal ,  Daqing Wang ,  Lakshml Bhagat ,  Dong Yu

发明人： Ekambar R. Kandimalla ,  Sudhir Agrawal ,  Daqing Wang ,  Lakshml Bhagat ,  Dong Yu

IPC分类号： A61K39/395 ,  A61P35/00 ,  A61K39/385 ,  A61K31/7088 ,  A61K39/00

CPC分类号： A61K39/0011 ,  A61K39/39558 ,  A61K2039/55561 ,  C07K16/2887 ,  C07K16/32 ,  C07K2317/24 ,  A61K2300/00

摘要： The invention relates to the therapeutic use of immunostimulatory oligonucleotides and/or immunomers in combination with chemotherapeutic agents to provide a synergistic therapeutic effect.

摘要翻译： 本发明涉及免疫刺激性寡核苷酸和/或免疫组合物与化学治疗剂组合以提供协同治疗效果的治疗用途。

公开(公告)号：US08153777B2

公开(公告)日：2012-04-10

申请号：US12612413

申请日：2009-11-04

申请人： Ekambar Kandimalla ,  Mallikarjuna Putta ,  Lakshmi Bhagat ,  Daqing Wang ,  Dong Yu ,  Sudhir Agrawal

发明人： Ekambar Kandimalla ,  Mallikarjuna Putta ,  Lakshmi Bhagat ,  Daqing Wang ,  Dong Yu ,  Sudhir Agrawal

IPC分类号： C07H21/04 ,  C07H21/02 ,  A61K48/00 ,  A61K39/395 ,  C12N15/63

CPC分类号： A61K39/39 ,  A61K2039/55561 ,  C07H21/04 ,  C12N15/1138 ,  C12N2310/11 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/414

摘要： Antisense oligonucleotide compounds, compositions and methods are provided for down regulating the expression of TLR5. The compositions comprise antisense oligonucleotides targeted to nucleic acids encoding TLR5. The compositions may also comprise antisense oligonucleotides targeted to nucleic acids encoding TLR5 in combination with other therapeutic and/or prophylactic compounds and/or compositions. Methods of using these compounds and compositions for down-regulating TLR5 expression and for prevention or treatment of diseases wherein modulation of TLR5 expression would be beneficial are provided.

摘要翻译： 提供反义寡核苷酸化合物，组合物和方法用于下调TLR5的表达。 组合物包含靶向编码TLR5的核酸的反义寡核苷酸。 组合物还可以包含靶向编码TLR5的核酸与其它治疗和/或预防化合物和/或组合物组合的反义寡核苷酸。 提供这些化合物和组合物用于下调TLR5表达和预防或治疗TLR5表达调节将是有益的疾病的方法。

公开(公告)号：US07851453B2

公开(公告)日：2010-12-14

申请号：US10757345

申请日：2004-01-14

申请人： Sudhir Agrawal ,  Lakshmi Bhagat ,  Dong Yu ,  Ekambar R. Kandimalla

发明人： Sudhir Agrawal ,  Lakshmi Bhagat ,  Dong Yu ,  Ekambar R. Kandimalla

IPC分类号： C07H21/02 ,  C07H21/04 ,  A01N43/04 ,  A61K31/70

CPC分类号： C12N15/117 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/111 ,  C12N2310/17 ,  C12N2310/33 ,  C12N2310/3519 ,  C12N2310/51

摘要： The invention relates to the therapeutic use of oligonucleotides as immunostimulatory agents in immunotherapy applications. More particularly, the invention provides immunomers and an immunostimulatory oligonucleotides for use in methods for generating an immune response or for treating a patient in need of immunostimulation. The immunomers and an immunostimulatory oligonucleotides of the invention preferably comprise novel purines. The immunomers according to the invention further comprise at least two oligonucleotides linked at their 3′ ends, internucleoside linkages or functionalized nucleobase or sugar to a non-nucleotidic linker, at least one of the oligonucleotides being an immunomodulatory oligonucleotide and having an accessible 5′ end.

摘要翻译： 本发明涉及在免疫治疗应用中寡核苷酸作为免疫刺激剂的治疗用途。 更具体地，本发明提供用于产生免疫应答的方法或用于治疗需要免疫刺激的患者的免疫学和免疫刺激性寡核苷酸。 本发明的免疫原和免疫刺激寡核苷酸优选包含新的嘌呤。 根据本发明的免疫反应物还包含至少两个在其3'末端连接的寡核苷酸，核苷间键或官能化核碱基或糖与非核酸连接体，至少一个寡核苷酸是免疫调节寡核苷酸并具有可接近的5'末端 。

公开(公告)号：US20100254974A1

公开(公告)日：2010-10-07

申请号：US12694844

申请日：2010-01-27

申请人： Sudhir Agrawal ,  Ekambar R. Kandimalla ,  Dong Yu ,  Lakshmi Bhagat

发明人： Sudhir Agrawal ,  Ekambar R. Kandimalla ,  Dong Yu ,  Lakshmi Bhagat

IPC分类号： A61K39/395 ,  A61K39/00 ,  A61P37/04 ,  A61P37/02

CPC分类号： C07H21/04 ,  A61K39/39 ,  A61K2039/55561

摘要： The invention relates to the therapeutic use of oligonucleotides as immunostimulatory agents in immunotherapy applications. More particularly, the invention provides immunomers for use in methods for generating an immune response or for treating a patient in need of immunostimulation. The immunomers of the invention comprise at least two oligonucleotides linked at their 3′ ends, internucleoside linkages or functionalized nucleobase or sugar to a non-nucleotidic linker, at least one of the oligonucleotides being an immunostimulatory oligonucleotide and having an accessible 5′ end.

摘要翻译： 本发明涉及在免疫治疗应用中寡核苷酸作为免疫刺激剂的治疗用途。 更具体地，本发明提供了用于产生免疫应答的方法或用于治疗需要免疫刺激的患者的免疫
性。 本发明的免疫体包含至少两个在其3'末端连接的寡核苷酸，核苷间键或官能化核碱基或糖与非核苷酸连接体，至少一个寡核苷酸是免疫刺激性寡核苷酸并具有可接近的5'端。

公开(公告)号：US07790168B2

公开(公告)日：2010-09-07

申请号：US10694207

申请日：2003-10-27

申请人： Ekambar R. Kandimalla ,  Qiuyan Zhao ,  Dong Yu ,  Sudhir Agrawal

发明人： Ekambar R. Kandimalla ,  Qiuyan Zhao ,  Dong Yu ,  Sudhir Agrawal

IPC分类号： A61K39/00 ,  A61K39/385 ,  A01N43/04 ,  C07H21/02

CPC分类号： C12N15/117 ,  A61K31/7088 ,  A61K31/7115 ,  A61K2039/55561 ,  C07H21/00 ,  C12N2310/18 ,  C12N2310/3125 ,  C12N2310/317 ,  C12N2310/318 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/33 ,  C12N2310/334 ,  C12N2310/336 ,  C12N2310/3521

摘要： The invention relates to the therapeutic use of oligonucleotides or oligonucleotide analogs as immunostimulatory agents in immunotherapy applications. The invention provides methods for enhancing the immune response caused by immunostimulatory oligonucleotide compounds.