公开(公告)号：US20060008407A1

公开(公告)日：2006-01-12

申请号：US10426578

申请日：2003-04-30

申请人： Richard Smalley ,  Daniel Colbert ,  Ting Guo ,  Andrew Rinzler ,  Pavel Nikolaev ,  Andreas Thess

发明人： Richard Smalley ,  Daniel Colbert ,  Ting Guo ,  Andrew Rinzler ,  Pavel Nikolaev ,  Andreas Thess

IPC分类号： D01F9/12

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  B82Y40/00 ,  C01B32/162 ,  C01B2202/02 ,  C01B2202/22 ,  C01B2202/36 ,  Y02P20/134 ,  Y10S428/903 ,  Y10S977/742 ,  Y10S977/743 ,  Y10S977/75 ,  Y10S977/751 ,  Y10S977/842 ,  Y10S977/844 ,  Y10S977/938 ,  Y10T428/2913 ,  Y10T442/50 ,  Y10T442/624

摘要： This invention provides a method of making single-wall carbon nanotubes by laser vaporizing a mixture of carbon and one or more Group VIII transition metals. Single-wall carbon nanotubes preferentially form in the vapor and the one or more Group VIII transition metals catalyzed growth of the single-wall carbon nanotubes. In one embodiment of the invention, one or more single-wall carbon nanotubes are fixed in a high temperature zone so that the one or more Group VIII transition metals catalyze further growth of the single-wall carbon nanotube that is maintained in the high temperature zone. In another embodiment, two separate laser pulses are utilized with the second pulse timed to be absorbed by the vapor created by the first pulse.

公开(公告)号：US20050100497A1

公开(公告)日：2005-05-12

申请号：US10426781

申请日：2003-04-30

申请人： Richard Smalley ,  Daniel Colbert ,  Ting Guo ,  Andrew Rinzler ,  Pavel Nikolaev ,  Andreas Thess

发明人： Richard Smalley ,  Daniel Colbert ,  Ting Guo ,  Andrew Rinzler ,  Pavel Nikolaev ,  Andreas Thess

IPC分类号： C01B31/02 ,  D01F9/12

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  B82Y40/00 ,  C01B32/162 ,  C01B2202/02 ,  C01B2202/22 ,  C01B2202/36 ,  Y02P20/134 ,  Y10S428/903 ,  Y10S977/742 ,  Y10S977/743 ,  Y10S977/75 ,  Y10S977/751 ,  Y10S977/842 ,  Y10S977/844 ,  Y10S977/938 ,  Y10T428/2913 ,  Y10T442/50 ,  Y10T442/624

摘要： This invention provides a method of making single-wall carbon nanotubes by laser vaporizing a mixture of carbon and one or more Group VIII transition metals. Single-wall carbon nanotubes preferentially form in the vapor and the one or more Group VIII transition metals catalyzed growth of the single-wall carbon nanotubes. In one embodiment of the invention, one or more single-wall carbon nanotubes are fixed in a high temperature zone so that the one or more Group VIII transition metals catalyze further growth of the single-wall carbon nanotube that is maintained in the high temperature zone. In another embodiment, two separate laser pulses are utilized with the second pulse timed to be absorbed by the vapor created by the first pulse.

摘要翻译： 本发明提供了一种通过激发汽化碳和一种或多种第八族过渡金属的混合物来制备单壁碳纳米管的方法。 单壁碳纳米管优先在蒸气中形成，并且一个或多个VIII族过渡金属催化单壁碳纳米管的生长。 在本发明的一个实施方案中，一个或多个单壁碳纳米管固定在高温区域中，使得一种或多种第VIII族过渡金属催化保持在高温区域中的单壁碳纳米管的进一步生长 。 在另一个实施例中，利用两个单独的激光脉冲，第二脉冲定时被第一脉冲产生的蒸气吸收。

公开(公告)号：US20050130260A1

公开(公告)日：2005-06-16

申请号：US10736448

申请日：2003-12-15

申请人： Lars Linden ,  Stefan Prytulla ,  Thomas Ostermann ,  Monika Baehner ,  Tilmann Roos ,  Andreas Thess ,  Hans Kiefer ,  Wolfgang Vogt

发明人： Lars Linden ,  Stefan Prytulla ,  Thomas Ostermann ,  Monika Baehner ,  Tilmann Roos ,  Andreas Thess ,  Hans Kiefer ,  Wolfgang Vogt

IPC分类号： C07K1/113 ,  C07K14/705 ,  C07K14/72 ,  C12P21/06

CPC分类号： C07K14/723 ,  C07K1/1136 ,  C07K14/705

摘要： The present invention relates to a method for preparing a solution of a refolded, recombinantly expressed or chemically synthesized eukaryotic membrane protein in monodisperse form, to methods for preparing a crystalline form of a recombinantly expressed or chemically synthesized membrane protein, to a crystalline form of a recombinantly expressed, or chemically synthesized eukaryotic membrane protein, and to a crystalline form of a complex of a recombinantly expressed or chemically synthesized eukaryotic membrane protein and of an accessory agent.

摘要翻译： 本发明涉及一种制备重折叠，重组表达或化学合成的单分散形式的真核细胞蛋白质的溶液的方法，用于制备重组表达或化学合成的膜蛋白的结晶形式的方法， 重组表达或化学合成的真核膜蛋白，以及重组表达或化学合成的真核细胞蛋白质和辅助剂的复合物的结晶形式。

公开(公告)号：US09234013B2

公开(公告)日：2016-01-12

申请号：US13321474

申请日：2011-08-12

申请人： Andreas Thess ,  Thomas Schlake ,  Jochen Probst

发明人： Andreas Thess ,  Thomas Schlake ,  Jochen Probst

IPC分类号： A61K48/00 ,  C07K14/435 ,  C12N15/67 ,  A61K38/02 ,  A61K38/03 ,  A61K38/04 ,  A61K38/10 ,  A61K38/16 ,  C07H21/04

CPC分类号： A61K48/0066 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/575 ,  C07K14/435 ,  C12N15/67

摘要： The present application describes a coding nucleic acid sequence, particularly a messenger RNA (mRNA), comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and the use thereof for increasing the expression of an encoded protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine e.g. for the use in the treatment of tumors and cancer diseases, cardiovascular diseases, infectious diseases, autoimmune diseases or genetic diseases, or in gene therapy. The present invention further describes an in vitro transcription method, in vitro methods for increasing the expression of a protein using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and an ex vivo and in vivo method.

摘要翻译： 本申请描述了编码核酸序列，特别是信使RNA（mRNA），其包含或编码组蛋白茎环和聚（A）序列或多聚腺苷酸化信号，以及其用于增加编码蛋白质的表达的用途 。 它还公开了其用于制备药物组合物，特别是疫苗的制剂。 用于治疗肿瘤和癌症疾病，心血管疾病，感染性疾病，自身免疫性疾病或遗传性疾病，或用于基因治疗。 本发明进一步描述体外转录方法，使用包含或编码组蛋白茎环和聚（A）序列或多聚腺苷酸化信号的核酸增加蛋白质表达的体外转录方法，以及离体和/ 体内法。

公开(公告)号：US20130129754A1

公开(公告)日：2013-05-23

申请号：US13321474

申请日：2011-08-12

申请人： Andreas Thess ,  Thomas Schlake ,  Jochen Probst

发明人： Andreas Thess ,  Thomas Schlake ,  Jochen Probst

IPC分类号： C07K14/435

CPC分类号： A61K48/0066 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/575 ,  C07K14/435 ,  C12N15/67

摘要： The present application describes a coding nucleic acid sequence, particularly a messenger RNA (mRNA), comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and the use thereof for increasing the expression of an encoded protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine e.g. for the use in the treatment of tumours and cancer diseases, cardiovascular diseases, infectious diseases, autoimmune diseases or genetic diseases, or in gene therapy. The present invention further describes an in vitro transcription method, in vitro methods for increasing the expression of a protein using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and an ex vivo and in vivo method.

摘要翻译： 本申请描述了编码核酸序列，特别是信使RNA（mRNA），其包含或编码组蛋白茎环和聚（A）序列或多聚腺苷酸化信号，以及其用于增加编码蛋白质的表达的用途 。 它还公开了其用于制备药物组合物，特别是疫苗的制剂。 用于治疗肿瘤和癌症疾病，心血管疾病，感染性疾病，自身免疫性疾病或遗传性疾病，或用于基因治疗。 本发明进一步描述体外转录方法，使用包含或编码组蛋白茎环和聚（A）序列或多聚腺苷酸化信号的核酸增加蛋白质表达的体外转录方法，以及离体和/ 体内法。

公开(公告)号：US20070031926A1

公开(公告)日：2007-02-08

申请号：US11453195

申请日：2006-06-14

申请人： Lars Linden ,  Stefan Prytulla ,  Thomas Ostermann ,  Monika Baehner ,  Tilmann Roos ,  Andreas Thess ,  Hans Kiefer ,  Wolfgang Vogt

发明人： Lars Linden ,  Stefan Prytulla ,  Thomas Ostermann ,  Monika Baehner ,  Tilmann Roos ,  Andreas Thess ,  Hans Kiefer ,  Wolfgang Vogt

IPC分类号： G06F19/00 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705

CPC分类号： C07K14/705

摘要： The present invention relates to a method for preparing a solution of a refolded, recombinantly expressed or chemically synthesized eukaryotic membrane protein in monodisperse form, to methods for preparing a crystalline form of a recombinantly expressed or chemically synthesized membrane protein, to a crystalline form of a recombinantly expressed, or chemically synthesized eukaryotic membrane protein, and to a crystalline form of a complex of a recombinantly expressed or chemically synthesized eukaryotic membrane protein and of an accessory agent.

摘要翻译： 本发明涉及一种制备重折叠，重组表达或化学合成的单分散形式的真核细胞蛋白质的溶液的方法，用于制备重组表达或化学合成的膜蛋白的结晶形式的方法， 重组表达或化学合成的真核膜蛋白，以及重组表达或化学合成的真核细胞蛋白质和辅助剂的复合物的结晶形式。