Screening the activity of drugs for central nervous system (CNS)

    公开(公告)号:US06541193B2

    公开(公告)日:2003-04-01

    申请号:US09789525

    申请日:2001-02-22

    IPC分类号: C12Q100

    CPC分类号: G01N33/94 G01N33/5082

    摘要: The invention relates to a method for screening of neuroactive drugs using the fruit fly Drosophila melanogaster, which comprises the steps of, generating a double mutant line of K+ channel genes in Drosophila melanogaster; culturing the Sh5eag1 mutant flies on Dorsophila medium under standard conditions; and anesthetizing flies with diethyl ether and observing the time taken by flies to recover from anesthesia.

    Method for maximization of artemisinin production by the plant artemisia annua
    54.
    发明授权
    Method for maximization of artemisinin production by the plant artemisia annua 失效
    青蒿植物青蒿素生产最大化的方法

    公开(公告)号:US06393763B1

    公开(公告)日:2002-05-28

    申请号:US09538892

    申请日:2000-03-30

    IPC分类号: A01B7900

    CPC分类号: A01H3/00

    摘要: The invention provides a method for maximization of artemisinin yield of the plant Artemisia annua, said method comprising sowing seeds of Artemisia annua plant on raised bed nursery during second and third week of December and maintaining the moisture throughout; transplanting seedlings thus obtained bearing at least 5-15 leaves into the main field fertilized with fertilizer, preferably NPK @ 80,40,40 kg/ha to achieve a population density of 50,000 to 200,000 per ha followed by light irrigation in the second week of March and irrigation every fortnight thereafter; harvesting the crop four times by cutting the plant tops leaving 75-100 cm part of plant for further regeneration, the said harvests are performed in a manner that the first harvest is done in fourth week of May, second harvest in third week of July, third harvest in second week of September and fourth harvest in third week of October of each year; and at each harvesting time care is taken to care at least one green branch, and extracting artemisinin from the plant tissue immediately after each harvest.

    摘要翻译: 本发明提供了一种使青蒿植物青蒿素产量最大化的方法,所述方法包括在12月的第二和第三周在秧苗上播种蒿种子,并保持水分; 将由此获得的至少5-15叶的幼苗移植到用肥料施肥的主田中,优选为80,40,40kg / ha的NPK,以实现每公顷50,000至20万的人口密度,然后在第二周进行轻度灌溉 三月份之后每两周灌溉一次; 通过切割植物顶部将作物收割四次,留下75-100cm部分的植物进行进一步再生,所述收获的进行方式是在5月的第4周进行第一次收获,在7月的第3周进行第二次收获, 9月第二周第三次收获,每年10月第三周收获第四季; 并且在每个收获时间处理至少一个绿色分支,并且在每次收获后立即从植物组织中提取青蒿素。

    Process for the preparation of maltol from plants belonging to the genus Abies
    55.
    发明授权
    Process for the preparation of maltol from plants belonging to the genus Abies 失效
    从属于Abies属的植物制备麦芽酚的方法

    公开(公告)号:US06384246B1

    公开(公告)日:2002-05-07

    申请号:US09792030

    申请日:2001-02-26

    IPC分类号: C07D30940

    CPC分类号: C07D309/40

    摘要: A process has been developed for production of a food flavoring compound maltol which comprises (a) extracting the dried and pulverized leaves of the plants belonging to the genus Abies with an alcohol at 20-40° C. and concentrating the solvent to obtain an alcoholic extract, (b) adsorbing the alcoholic extract with an adsorbent and drying the adsorbed material at a temperature ranging between 20-50° C., (d) partitioning of the adsorbed material between selected solvents consisting of aliphatic hydrocarbon and chlorinated solvent successively, (d) concentrating the chlorinated solvent to a residue and crystallizing it from a suitable organic solvent or mixtures of such solvents to get pure maltol.

    摘要翻译: 已经开发了用于生产食品香料化合物麦芽酚的方法,其包括(a)在20-40℃下用醇在20-40℃下提取属于Abies属的植物的干燥和粉碎的叶子并浓缩溶剂以获得酒精 提取物,(b)用吸附剂吸附醇提取物并在20-50℃的温度范围内干燥吸附的材料,(d)连续地将由脂族烃和氯化溶剂组成的选定溶剂之间的吸附材料分配(( d)将氯化溶剂浓缩至残余物并将其从合适的有机溶剂或这些溶剂的混合物中结晶得到纯的麦芽酚。

    Tissue culture process for producing a large number of viable mint plants in vitro
    58.
    发明授权
    Tissue culture process for producing a large number of viable mint plants in vitro 失效
    用于在体外生产大量活的薄荷植物的组织培养方法

    公开(公告)号:US06323394B1

    公开(公告)日:2001-11-27

    申请号:US09263485

    申请日:1999-03-08

    IPC分类号: A01H100

    摘要: The present invention relates to a tissue culture process for producing a large number of genetically transformed viable mint plants in vitro. The process of the present invention employs specified pieces of an internodal segment of the stem of the mint plant as the starting material and identifies medium, culture and transformation conditions for producing a large number of genetically transformed plants. Such plants can be selected for desirable characteristics.

    摘要翻译: 本发明涉及用于在体外生产大量遗传转化的活的薄荷植物的组织培养方法。 本发明的方法使用薄荷植物茎的节间段的指定片段作为起始材料,并鉴定用于生产大量遗传转化植物的培养和转化条件。 可以选择这样的植物用于期望的特征。