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    SUBSTRATE AND METHOD FOR CULTURING BREAST CELLS
    52.
    发明申请
    SUBSTRATE AND METHOD FOR CULTURING BREAST CELLS 审中-公开
    用于培养乳腺细胞的基质和方法

    公开(公告)号：US20100297675A1

    公开(公告)日：2010-11-25

    申请号：US12623884

    申请日：2009-11-23

    申请人： Huayun Deng ,  Joydeep Lahiri ,  Hui Su

    发明人： Huayun Deng ,  Joydeep Lahiri ,  Hui Su

    IPC分类号： G01N33/574 ,  C12N5/07

    CPC分类号： C12N5/0631 ,  C12N5/0693 ,  C12N2503/02 ,  C12N2506/30 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2535/00 ,  G01N33/5011 ,  G01N33/57415

    摘要： A cell culture article includes a porous substrate having a plurality of pores and a plurality of interstices in communication with the pores. At least some of the plurality of pores and interstices are sufficiently large for two or more mammary epithelial cells to cluster within the pores or interstices. Non-malignant mammary epithelial cells or breast cancer cells may not attach strongly to the substrate surface, which may encourage cell-cell interaction. In many cases, the article is desirably free of components of unknown origin. The articles may be capable of maintaining culture of malignant and non-malignant mammary epithelial cells and allowing for development of in vivo-like morphologies or characteristics of such cells.

    摘要翻译： 细胞培养物包括具有多个孔和与孔连通的多个间隙的多孔基材。 多个孔和间隙中的至少一些孔和间隙对于两个或更多个乳腺上皮细胞聚集在孔或间隙内是足够大的。 非恶性乳腺上皮细胞或乳腺癌细胞可能不会强烈附着于底物表面，这可能促进细胞 - 细胞相互作用。 在许多情况下，该物品理想地不含未知来源的成分。 这些制品可能能够维持恶性和非恶性乳腺上皮细胞的培养，并允许发展体内样的形态或这些细胞的特征。

    Polymer-coated substrates for binding biological molecules
    53.
    发明申请
    Polymer-coated substrates for binding biological molecules 审中-公开
    用于结合生物分子的聚合物涂覆的底物

    公开(公告)号：US20060257919A1

    公开(公告)日：2006-11-16

    申请号：US11488228

    申请日：2006-07-18

    申请人： Anthony Frutos ,  Joydeep Lahiri ,  Thomas Leslie ,  Jinlin Peng

    发明人： Anthony Frutos ,  Joydeep Lahiri ,  Thomas Leslie ,  Jinlin Peng

    IPC分类号： C40B40/08 ,  C40B50/14

    CPC分类号： B82Y30/00 ,  G01N33/54393 ,  G01N33/548

    摘要： A substrate, which that is capable of attaching biomolecules, and a method for preparing the substrate are provided. The substrate has a reactive surface that can covalently attach a polymer coating containing functional groups, which can reduce nonspecific binding of biomolecules to the surface for a biological array. Optionally, at least a portion of the substrate may be coated with an intermediate tie layer, which enhances the covalent bonding between the polymer coating with the underlying substrate. The present invention also pertains to a method that uses electrostatic blocking agents to reduce non-specific binding of proteins to a substrate, especially anhydride-modified surfaces.

    摘要翻译： 提供了能够附着生物分子的基材和制备基材的方法。 基底具有可以共价连接含有官能团的聚合物涂层的反应性表面，其可以降低生物分子与生物分子的表面的非特异性结合。 任选地，衬底的至少一部分可以涂覆有中间粘结层，其增强聚合物涂层与下面的衬底之间的共价键。 本发明还涉及使用静电阻断剂以减少蛋白质与底物，特别是酸酐改性表面的非特异性结合的方法。