Porous substrates for DNA arrays
    3.
    发明授权
    Porous substrates for DNA arrays 失效
    用于DNA阵列的多孔底物

    公开(公告)号:US06994972B2

    公开(公告)日:2006-02-07

    申请号:US10101144

    申请日:2002-03-18

    摘要: A planar, rigid substrate made from a porous, inorganic material coated with cationic polymer molecules for attachment of an array of biomolecules, such as DNA, RNA, oligonucleotides, peptides, and proteins. The substrate has a top surface with about at least 200 to about 200,000 times greater surface area than that of a comparable, non-porous substrate. The cationic polymer molecules are anchored on the top surface and in the pores of the porous material. In high-density applications, an array of polynucleotides of a known, predetermined sequence is attached to this cationic polymer layer, such that each of the polynucleotide is attached to a different localized area on the top surface. The top surface has a surface area for attaching biomolecules of approximately 387,500 cm2/cm2 of area (˜7.5 million cm2/1×3 inch piece of substrate). Each pore of the plurality of pores in the top surface of the substrate has a pore radius of between about 40 Å to about 75 Å. Not only does the cationic coating in and over the pores of the substrate greatly increase the overall positive charge on the substrate surface, but also given the size of the pores provides binding sites to which biomolecules can better attach.

    摘要翻译: 由用于附着生物分子阵列如DNA,RNA,寡核苷酸,肽和蛋白质的阳离子聚合物分子的多孔无机材料制成的平面刚性基底。 衬底具有比可比较的非多孔衬底的表面面积大至少约200至约200,000倍的顶表面。 阳离子聚合物分子锚定在多孔材料的顶表面和孔中。 在高密度应用中,将已知的预定序列的多核苷酸阵列连接到该阳离子聚合物层上,使得每个多核苷酸连接到顶表面上的不同局部区域。 顶表面具有用于附着大约387,500cm 2 / cm 2的面积(约750万厘米2/1/3英寸)的生物分子的表面积 基片)。 衬底的顶表面中的多个孔的每个孔的孔半径在大约40埃至大约75埃之间。 衬底中的阳离子涂层不仅大大增加了衬底表面上的总体正电荷,而且还给出了孔的尺寸提供生物分子可以更好地附着的结合位点。