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    Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
    54.
    发明授权
    Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof 有权
    委内瑞兰马脑炎病毒复制子与基因组中的适应性突变及其用途

    公开(公告)号：US07332322B2

    公开(公告)日：2008-02-19

    申请号：US11227671

    申请日：2005-09-14

    申请人： Ilya Frolov ,  Elena Frolova

    发明人： Ilya Frolov ,  Elena Frolova

    IPC分类号： C12N7/04 ,  C12N15/86 ,  C12P21/04 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N7/00 ,  C12N2770/36121 ,  C12N2770/36143

    摘要： The present invention provides a Venezuelan equine encephalitis virus replicon RNA useful in the development of stable lines of mammalian, avian and insect cells in which these replicons will persistently replicate. Venezuelan equine encephalitis (VEE) virus replicons contain a number of unique adaptive mutations that make the replicons noncytopathic. The replicons remain resistant to IFN-α/β. Replicon replication leads to high-level production of heterologous proteins, which are encoded by the replicons' genome and are under the control of a viral subgenomic promoter. Also provided are methods of screening for inhibitory compounds of Venezuelan equine encephalitis virus replication and eastern equine encephalitis virus replication.

    摘要翻译： 本发明提供了一种可用于发展哺乳动物，禽和昆虫细胞的稳定系的委内瑞兰马脑炎病毒复制子RNA，其中这些复制子将持续复制。 委内瑞兰马脑炎（VEE）病毒复制子包含许多独特的适应性突变，使复制子非细胞病变。 复制子对IFN-α/β仍然具有抗性。 复制子复制导致异源蛋白质的高水平生产，其由复制子基因组编码并在病毒亚基因组启动子的控制下。 还提供了筛选委内瑞兰马脑炎病毒复制和东马脑炎病毒复制的抑制性化合物的方法。

    Vectors derived from South African Arbovirus No. 86
    55.
    发明授权
    Vectors derived from South African Arbovirus No. 86 有权
    衍生自南非Arbovirus No.86的载体

    公开(公告)号：US06982087B2

    公开(公告)日：2006-01-03

    申请号：US10363603

    申请日：2001-09-06

    申请人： Robert E. Johnston ,  Mark T. Heise ,  Dennis Simpson

    发明人： Robert E. Johnston ,  Mark T. Heise ,  Dennis Simpson

    IPC分类号： A61K39/12 ,  C12N7/01 ,  C12N15/40 ,  C12N15/86

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/525 ,  A61K2039/5254 ,  A61K2039/53 ,  C12N7/00 ,  C12N9/506 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2770/36162

    摘要： Provided herein are alphavirus vectors derived from South African Arbovirus No. 86 (S.A.AR86) comprising attenuating mutations and methods of making the same. Also provided are improved viral vectors and helper constructs comprising a S.A.AR86 capsid enhancer sequence. The present invention also provides S.A.AR86 replicon and helper constructs comprising an alphavirus capsid enhancer sequence. Further provided are methods of administering an alphavirus vector comprising a heterologous nucleotide sequence (preferably encoding an immunogen or a therapeutic polypeptide) according to the invention to a cell or subject. In preferred embodiments, the alphavirus vector delivers the heterologous nucleotide sequence to the cells of the bone, bone marrow, and/or bone-associated connective tissue.

    摘要翻译： 本文提供了衍生自南非野花病毒第86号（S.A.AR86）的甲病毒载体，其包含减毒突变及其制备方法。 还提供了包含S.A.AR86衣壳增强子序列的改进的病毒载体和辅助构建体。 本发明还提供了包含甲病毒衣壳增强子序列的S.A.AR86复制子和辅助构建体。 还提供了向细胞或受试者施用包含根据本发明的异源核苷酸序列（优选编码免疫原或治疗性多肽）的甲病毒载体的方法。 在优选的实施方案中，甲病毒载体将异源核苷酸序列递送至骨，骨髓和/或骨相关结缔组织的细胞。