公开(公告)号：US11198857B2

公开(公告)日：2021-12-14

申请号：US17388358

申请日：2021-07-29

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20210381007A1

公开(公告)日：2021-12-09

申请号：US17338603

申请日：2021-06-03

Applicant: Inscripta, Inc.

Inventor： Janine Mok ,  Miles Gander ,  Andrew Garst ,  Burak Dura ,  Deanna Church

IPC: C12N15/90 ,  C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods and modules to edit live cells and to subsequently correlate the resulting cellular nucleic acids of the edited cells to the edits.

公开(公告)号：US11174471B2

公开(公告)日：2021-11-16

申请号：US17200089

申请日：2021-03-12

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Andrew Garst ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：US11104890B1

公开(公告)日：2021-08-31

申请号：US17230765

申请日：2021-04-14

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US11053485B2

公开(公告)日：2021-07-06

申请号：US17084522

申请日：2020-10-29

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Andrew Garst ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：US20210198641A1

公开(公告)日：2021-07-01

申请号：US17200074

申请日：2021-03-12

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Andrew Garst ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：US20210180090A1

公开(公告)日：2021-06-17

申请号：US17179193

申请日：2021-02-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20210163877A1

公开(公告)日：2021-06-03

申请号：US17175612

申请日：2021-02-13

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12N15/87 ,  C12M1/26 ,  C12M3/00 ,  B01D71/02 ,  B01D63/08 ,  B01D69/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20210108171A1

公开(公告)日：2021-04-15

申请号：US17102414

申请日：2020-11-23

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20210031144A1

公开(公告)日：2021-02-04

申请号：US17073300

申请日：2020-10-17

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Matthew Estes ,  Bruce Chabansky ,  Andrew Garst ,  Richard Fox ,  Jorge Bernate

IPC: B01D63/08

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.