公开(公告)号：US11697826B2

公开(公告)日：2023-07-11

申请号：US16421783

申请日：2019-05-24

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113 ,  C07K14/195

CPC classification number: C12N15/902 ,  C07K14/195 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C07K2319/85 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11584928B2

公开(公告)日：2023-02-21

申请号：US16295393

申请日：2019-03-07

Applicant: The Regents of the University of Colorado ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20210388391A1

公开(公告)日：2021-12-16

申请号：US17387860

申请日：2021-07-28

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20230227810A1

公开(公告)日：2023-07-20

申请号：US18157740

申请日：2023-01-20

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/11 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/1079 ,  C12N2310/20

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US11130970B2

公开(公告)日：2021-09-28

申请号：US17179193

申请日：2021-02-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20190194650A1

公开(公告)日：2019-06-27

申请号：US16295393

申请日：2019-03-07

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US10287575B2

公开(公告)日：2019-05-14

申请号：US15948798

申请日：2018-04-09

Applicant: The Regents of the University of Colorado ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US09982279B1

公开(公告)日：2018-05-29

申请号：US15632001

申请日：2017-06-23

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/85 ,  C12N9/22 ,  C12N5/10 ,  C12N5/14 ,  C12N5/16 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20250075232A1

公开(公告)日：2025-03-06

申请号：US18953294

申请日：2024-11-20

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US10435714B2

公开(公告)日：2019-10-08

申请号：US15896433

申请日：2018-02-14

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/82 ,  C12N15/85 ,  C12N9/22 ,  C12N5/14 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81 ,  C12N15/113

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.