公开(公告)号：US07223541B2

公开(公告)日：2007-05-29

申请号：US10358818

申请日：2003-02-05

申请人： Carl Fuller ,  Shiv Kumar ,  Anup Sood ,  John Nelson

发明人： Carl Fuller ,  Shiv Kumar ,  Anup Sood ,  John Nelson

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6823 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q2521/525

摘要： The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

摘要翻译： 本发明涉及使用标记的底物或底物类似物检测靶的改进方法。 所述方法包括在酶催化的反应中使底物或底物类似物反应，其仅在这种底物或底物类似物反应时产生具有独立可检测信号的标记部分。 本发明特别地描述了基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物来检测样品中核酸的方法。 本发明提供的方法利用了具有比色染料，化学发光或荧光部分的核苷多磷酸，双脱氧核苷多聚磷酸酯或脱氧核苷多聚磷酸酯类似物，连接至末端磷酸酯的质量标签或电化学标签。 当核酸聚合酶使用该类似物作为底物时，酶活性标记将存在于磷酸转移的无机多磷酸盐副产物上。 通过磷酸酶切割磷酸转移的多磷酸盐产物导致附着在其上的标签的可检测的变化。 当在磷酸酶的存在下进行聚合酶测定时，提供了用于实时监测DNA或RNA合成和检测靶核酸的方便的方法。

公开(公告)号：US20070044975A1

公开(公告)日：2007-03-01

申请号：US11508689

申请日：2006-08-23

申请人： Thomas Fraser ,  Charles Jennings ,  John Nelson

发明人： Thomas Fraser ,  Charles Jennings ,  John Nelson

IPC分类号： E21B17/01

CPC分类号： E21B17/01 ,  E21B17/085 ,  E21B19/006 ,  E21B19/16 ,  Y10S285/922 ,  Y10T29/49947

摘要： An offshore riser system has riser joints, each having a pin and a box. The pin has an external grooved profile that is engaged by a locking element carried by the box of another riser joint. An actuating ring engages with the locking element to move it into the locked position. A retractable spider supports the string of riser while the new joint is being made up. A makeup tool on the riser deploying floor moves the ring relative to the locking element, causing the locking element to move to the locked position.

摘要翻译： 海上立管系统具有立管接头，每个接头都有一个销和一个箱体。 销具有外部槽形轮廓，其由另一提升管接头的盒承载的锁定元件接合。 致动环与锁定元件接合以将其移动到锁定位置。 可伸缩的蜘蛛支撑新的接头正在组成的立管。 提升器部署地板上的化妆工具相对于锁定元件移动环，导致锁定元件移动到锁定位置。

公开(公告)号：US20060272656A1

公开(公告)日：2006-12-07

申请号：US11143930

申请日：2005-06-01

申请人： John Nelson ,  Vernon Barnes

发明人： John Nelson ,  Vernon Barnes

IPC分类号： A24C5/00

CPC分类号： A24C5/06 ,  A24C5/40

摘要： A device and method for loading a cartridge with pre-formed tubular wrappers for cigarette manufacture. The device provides for dispensing pre-formed tubular wrappers from a container to a cartridge without directly touching the wrappers.

摘要翻译： 用于装载用于卷烟制造的预成型管状包装纸盒的装置和方法。 该装置用于将预先形成的管状包装从容器分配到药筒而不直接接触包装纸。

公开(公告)号：US20060272655A1

公开(公告)日：2006-12-07

申请号：US11375700

申请日：2006-03-14

申请人： Timothy Thomas ,  Vernon Barnes ,  John Nelson ,  August Borschke

发明人： Timothy Thomas ,  Vernon Barnes ,  John Nelson ,  August Borschke

IPC分类号： A24C5/00

CPC分类号： A24C5/40 ,  A24C5/06 ,  A24C5/12 ,  B26D1/16 ,  B26D3/16 ,  B26D7/01

摘要： An apparatus and method for manufacturing small quantities of cigarettes in a substantially simultaneous fashion while maintaining consistent quality between the cigarettes. The invention provides an apparatus and method for simultaneously inserting charges of tobacco into pre-formed tubular cigarette wrappers.

公开(公告)号：US20060191680A1

公开(公告)日：2006-08-31

申请号：US11348201

申请日：2006-02-06

申请人： John Nelson

发明人： John Nelson

IPC分类号： E21B43/10

CPC分类号： E21B33/043 ,  E21B2033/005

摘要： A method of completing a well having a casing hanger set in a subsea wellhead housing includes attaching a running tool to a tubular bridging hanger. A metal-to-metal inner seal is attached to a lower exterior portion of the bridging hanger and a metal-to-metal outer seal is located on an upper exterior portion of the bridging hanger. The assembly is lowered into the well and the lower exterior portion of the bridging hanger is inserted into the casing hanger. The inner seal is wedged between the casing hanger and the bridging hanger in response to weight of the running string. The running tool is actuated to set the outer seal between the upper exterior portion of the bridging hanger and the wellhead housing. Then, a tubing hanger is landed and sealed in the interior of the bridging hanger.

摘要翻译： 完成具有设置在海底井口壳体中的套管悬挂器的井的方法包括将运行的工具附接到管状桥接悬挂器。 金属对金属内部密封件附接到桥接悬挂器的下部外部部分，并且金属对金属外部密封件位于桥接悬挂器的上部外部部分上。 将组件下降到井中，桥接衣架的下部外部部分插入到衣架中。 响应于运行弦的重量，内密封件楔入套管吊架和桥接衣架之间。 运行工具被致动以将外部密封件设置在桥接衣架的上部外部部分与井口壳体之间。 然后，管道悬挂器着陆并密封在桥接衣架的内部。

公开(公告)号：US20050246275A1

公开(公告)日：2005-11-03

申请号：US10862697

申请日：2004-06-07

申请人： John Nelson

发明人： John Nelson

IPC分类号： G06Q30/00 ,  G06F17/60

CPC分类号： G06Q30/06 ,  G06Q20/102

摘要： An apparatus, system and method is disclosed that solves the needs in the art by providing an apparatus, method and system to accomplish transactions between aircraft customers (buyers) and FBO office (seller) to be sold, paid, billed and accounted in real-time, both remotely at the aircraft location on the airport by a remote operator and inside the FBO office by an inside operator.

摘要翻译： 公开了一种通过提供一种装置，方法和系统来实现飞机客户（买方）和FBO办公室（卖方）之间交易的设备，系统和方法，以便实时出售，支付，计费和核算交易。 时间，远程操作员在机场的飞机位置，以及内部操作员在FBO办公室内。

公开(公告)号：US20050128579A1

公开(公告)日：2005-06-16

申请号：US10733066

申请日：2003-12-11

申请人： Patrick Thomas ,  John Nelson ,  Scott Tapio ,  Michael Graetz ,  Amy Gates ,  Peter Olofson ,  Robert Brott ,  Robert Moshrefzadeh

发明人： Patrick Thomas ,  John Nelson ,  Scott Tapio ,  Michael Graetz ,  Amy Gates ,  Peter Olofson ,  Robert Brott ,  Robert Moshrefzadeh

IPC分类号： B29C70/74 ,  B29D11/00 ,  G03B21/56 ,  G03B21/62

CPC分类号： B29C70/745 ,  B29D11/00278 ,  B29D11/0074 ,  G03B21/625

摘要： A film for a screen has a light transmitting substrate and a plurality of structures disposed on the substrate. An optical material at least partially fills cavities between the structures. A method of forming a film includes providing a light transmitting substrate having a plurality of structures disposed thereon and at least partially filling the cavities therebetween with an optical material.

摘要翻译： 用于屏幕的膜具有透光基板和设置在基板上的多个结构。 光学材料至少部分地填充结构之间的空腔。 一种形成膜的方法包括提供一种透光基底，其具有设置在其上的多个结构，并且用光学材料至少部分地填充其间的空腔。

公开(公告)号：US20050076925A1

公开(公告)日：2005-04-14

申请号：US10682570

申请日：2003-10-09

申请人： Barry Fagg ,  Vernon Barnes ,  Sydney Seymour ,  Lloyd Hancock ,  John Nelson ,  Carl Greene ,  Balager Ademe

发明人： Barry Fagg ,  Vernon Barnes ,  Sydney Seymour ,  Lloyd Hancock ,  John Nelson ,  Carl Greene ,  Balager Ademe

IPC分类号： A24C1/26 ,  A24C5/00 ,  A24C5/32

CPC分类号： A24D1/025 ,  A24C5/005 ,  A24C5/20

摘要： Cigarettes are manufactured using modified automated cigarette making apparatus. Those cigarettes possess smokable rods having paper wrapping materials having additive materials applied thereto as patterns. The additive materials, which can have the forms of liquid or paste formulations (e.g., aqueous formulations incorporating starch or modified starch), are applied to a continuous paper web on the cigarette making apparatus. The formulation is applied to the paper web using application apparatus possessing rollers a series of roller. For example, additive material is applied to the roll face of a transfer roller due to roll interaction of that transfer roller with a pick-up roller; roll interaction of the transfer roller with an application roller causes transfer of the additive material from the transfer roller to the application roller; and additive material from the application roller is transferred to the paper web that passes between the application roller and a back-up roller. A radiant dryer is used to dry the additive material that has been applied to the paper web. The radiant dryer is located on one component of a two component assembly that is used to manufacture cigarettes. A first component of the two component assembly provides a source of paper web, applies additive material to that web in a pattern and dries the paper web; while a second component receives the paper web, supplies tobacco filler and manufactures a cigarette rod from the paper web and tobacco filler. An alternate assembly system provides a source of paper web, applies additive material to that web in a pattern, dries the paper web, and winds the treated paper web onto a bobbin; and that bobbin is later used to provide a source of paper web on a cigarette making machine unrolls that bobbin, receives the treated paper web from that bobbin, supplies tobacco filler and manufactures a cigarette rod from the paper web and tobacco filler. Spectrometric techniques are used to ensure proper registration of the additive material on the cigarette rods so manufactured, and to ensure proper quality of those cigarettes.

公开(公告)号：US20050039764A1

公开(公告)日：2005-02-24

申请号：US10645997

申请日：2003-08-22

申请人： Vernon Barnes ,  Philip Deal ,  Lloyd Hancock ,  John Nelson

发明人： Vernon Barnes ,  Philip Deal ,  Lloyd Hancock ,  John Nelson

IPC分类号： A24C1/26 ,  A24C5/00 ,  A24C5/32

CPC分类号： A24D1/025 ,  A24C5/005 ,  A24C5/20

摘要： Cigarettes are manufactured using modified automated cigarette making apparatus. Those cigarettes possess smokable rods having paper wrapping materials having additive materials applied thereto as patterns. The additive materials, which can have the forms of liquid or paste formulations (e.g., aqueous formulations incorporating starch or modified starch), are applied to a continuous paper web on the cigarette making apparatus. The formulation is applied to the paper web using application apparatus possessing rollers, and one of those rollers has a series of pockets in its roll face to receive additive formulation from a reservoir and to define the pattern of the formulation on the paper. For example, additive material located in the recessed pockets of a first roller is transferred in a controlled manner to the roll face of a second roller in roll contact with that first roller; and the additive material on the roll face of the second roller is transferred to desired locations on the surface of the paper web. The formulation also can be applied to a continuous moving paper web using an application apparatus possessing four rollers. For example, additive material is applied to the roll face of a transfer roller due to roll interaction of that transfer roller with a pick-up roller; roll interaction of the transfer roller with an application roller causes transfer of the additive material from the transfer roller to the application roller; and additive material from the application roller is transferred to the paper web that passes between the application roller and a back-up roller. A radiant dryer is used to dry the additive material that has been applied to the paper web. The radiant dryer is located on one component of a two component assembly that is used to manufacture cigarettes. A first component of the two component assembly provides a source of paper web, applies additive material to that web in a pattern and dries the paper web; while a second component receives the paper web, supplies tobacco filler and manufactures a cigarette rod from the paper web and tobacco filler. Spectrometric techniques are used to ensure proper registration of the additive material on the cigarette rods so manufactured, and to ensure proper quality of those cigarettes.

公开(公告)号：US06323009B1

公开(公告)日：2001-11-27

申请号：US09605192

申请日：2000-06-28

申请人： Roger S. Lasken ,  Frank B. Dean ,  John Nelson

发明人： Roger S. Lasken ,  Frank B. Dean ,  John Nelson

IPC分类号： C12P1934

CPC分类号： C12Q1/6844 ,  C12Q2537/143 ,  C12Q2531/125

摘要： Processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences. A kit containing components for use in the invention is also described.

摘要翻译： 公开了使用多个特异性和/或随机序列寡核苷酸引物扩增单链或双链环状DNA分子，特别是存在于菌落和斑块提取物中的靶DNA序列的方法以及用于检测这些扩增的靶序列 。 还描述了包含用于本发明的组分的试剂盒。