公开(公告)号：US20140315310A1

公开(公告)日：2014-10-23

申请号：US14105994

申请日：2013-12-13

Applicant: Massachusetts Institute of Technology

Inventor： Timothy Kuan-Ta Lu ,  Piro Siuti

IPC: G06N3/00

CPC classification number: G06N3/002

Abstract: The invention provides, inter alia, recombinase-based systems that provide for integrated logic and memory in living cells.

Abstract translation: 本发明尤其提供了提供活细胞中的集成逻辑和记忆的基于重组酶的系统。

公开(公告)号：US08795958B2

公开(公告)日：2014-08-05

申请号：US13661169

申请日：2012-10-26

Applicant: Trustees of Boston University ,  Massachusetts Institute of Technology

Inventor： James J. Collins ,  Timothy Kuan-Ta Lu

IPC: C12Q1/68

CPC classification number: C12N15/1086 ,  C12N15/111 ,  C12N15/63 ,  C12N2310/111 ,  C12N2320/50

Abstract: Described are methods and compositions for the detection of target genes. The inventors have developed a synthetic nucleic acid sensor-effector gene circuit. In cells without a target gene, the circuit suppresses e.g., effector production, but in the presence of the target gene the suppression is subject to competition, such that the synthetic sensor is de-repressed and permits expression of the effector gene. The methods and compositions described further permit the selective expression of an effector gene in those cells expressing the target gene. In this manner, cells expressing a target gene can be selectively targeted for treatment or elimination. In certain aspects, the methods and compositions described permit the selective expression of an agent such as a therapeutic gene product, in a specifically targeted population of cells in an organism.

Abstract translation: 描述了用于检测靶基因的方法和组合物。 本发明人开发了合成核酸传感器 - 效应子基因电路。 在没有靶基因的细胞中，电路抑制例如效应子产生，但是在靶基因的存在下，抑制受到竞争，使得合成传感器被去抑制并且允许效应基因的表达。 所描述的方法和组合物进一步允许在表达靶基因的那些细胞中选择性表达效应基因。 以这种方式，表达靶基因的细胞可以选择性地靶向用于治疗或消除。 在某些方面，所描述的方法和组合允许在生物体中的特定靶细胞群中选择性地表达诸如治疗性基因产物的试剂。