公开(公告)号：US09863864B2

公开(公告)日：2018-01-09

申请号：US15264326

申请日：2016-09-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Daniel Nelson Fox

IPC: G01N21/00 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N15/1429 ,  G01N15/1425 ,  G01N15/147 ,  G01N2015/1006

Abstract: Disclosed is a threshold selector that selects a threshold by generating a histogram from a detector signal and a flow cytometer. The detector signal includes both data and noise. A histogram is generated, which includes height data from the pulses of the detector signal as well as noise. The flow cytometer can be operated without samples to generate a histogram that includes only noise. The threshold signal can then be selected by selecting an intensity level on the histogram that is between the noise and data.