公开(公告)号：US20230242882A1

公开(公告)日：2023-08-03

申请号：US17998563

申请日：2021-05-17

申请人： MYORIDGE CO. LTD.

发明人： Itsunari Minami ,  Itsuro Motegi ,  Koji Eto

IPC分类号： C12N5/078

CPC分类号： C12N5/0644 ,  C12N2506/1346 ,  C12N2506/45 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2500/90

摘要： A method for producing a target cell includes producing a target cell by culturing a raw material cell in a serum-free medium containing: at least one culture factor selected from a growth factor and a differentiation inducing factor; human serum albumin; and at least one additive selected from a group consisting of a Src inhibitor, a PKC activator, methylcellulose, an Essential 8 medium, recombinant human serum albumin, and non-human animal serum albumin.

公开(公告)号：US11726083B2

公开(公告)日：2023-08-15

申请号：US16957625

申请日：2018-12-26

申请人： Myoridge Co. Ltd.

发明人： Shinichi Sueta ,  Itsunari Minami ,  Tomoko Kasahara ,  Kentaro Ishida ,  Naohiro Makita

IPC分类号： C12Q1/02 ,  G01N33/50 ,  G01N33/84

CPC分类号： G01N33/5061 ,  G01N33/84 ,  G01N2500/10

摘要： A method for testing drug response of a cardiomyocyte, the method comprising: testing a response of the cardiomyocyte to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less; or testing a response of the cardiomyocyte to an added drug immediately after placing the cardiomyocyte in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less.

公开(公告)号：US20240309327A1

公开(公告)日：2024-09-19

申请号：US18411134

申请日：2024-01-12

申请人： MITSUBISHI CHEMICAL CORPORATION ,  MYORIDGE CO. LTD.

发明人： Itsunari MINAMI ,  Kazuma SHOJI ,  Michiko YAMAZAKI ,  Hiroshi KAWABATA

IPC分类号： C12N5/077 ,  C12N5/00

CPC分类号： C12N5/0657 ,  C12N5/0037 ,  C12N5/0056 ,  C12N2501/998 ,  C12N2506/45

摘要： The present invention relates to a method for producing a cardiomyocyte. In more detail, the present invention relates to a method for producing a cell population containing cardiomyocytes, including differentiation process from cells capable of differentiating into cardiomyocytes to cardiomyocytes, and the differentiation process includes culturing the cells in a serum-free medium containing α fetoprotein to obtain the cardiomyocytes.

公开(公告)号：US20210372993A1

公开(公告)日：2021-12-02

申请号：US16957625

申请日：2018-12-26

申请人： Myoridge Co. Ltd.

发明人： Shinichi Sueta ,  Itsunari Minami ,  Tomoko Kasahara ,  Kentaro Ishida ,  Naohiro Makita

IPC分类号： G01N33/50 ,  G01N33/84

摘要： A method for testing drug response of a cardiomyocyte, the method comprising: testing a response of the cardiomyocyte to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less; or testing a response of the cardiomyocyte to an added drug immediately after placing the cardiomyocyte in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less.

公开(公告)号：US20230408495A1

公开(公告)日：2023-12-21

申请号：US18337884

申请日：2023-06-20

申请人： MYORIDGE CO. LTD.

发明人： Shinichi Sueta ,  Itsunari Minami ,  Tomoko Kasahara ,  Kentaro Ishida ,  Naohiro Makita

IPC分类号： G01N33/50 ,  G01N33/84

CPC分类号： G01N33/5061 ,  G01N33/84 ,  G01N2500/10

摘要： A method for testing drug response of a cardiomyocyte, the method comprising: testing a response of the cardiomyocyte to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less; or testing a response of the cardiomyocyte to an added drug immediately after placing the cardiomyocyte in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less.

公开(公告)号：US20230242885A1

公开(公告)日：2023-08-03

申请号：US18001058

申请日：2021-06-08

申请人： Myoridge Co. Ltd.

发明人： Toyomi Nakamura ,  Saori Fujikawa ,  Maiko Minatohara ,  Itsunari Minami

IPC分类号： C12N5/077 ,  A61L27/38

CPC分类号： C12N5/0657 ,  A61L27/38 ,  C12N2501/415 ,  C12N2501/11 ,  C12N2506/45

摘要： A method of producing a cardiomyocyte population, the method comprising culturing a cell population containing cardiomyocytes seeded at a cell density of 0.1×105 to 5.0×105 cells/cm2 in a culture solution to proliferate the cardiomyocytes.

公开(公告)号：USD998820S1

公开(公告)日：2023-09-12

申请号：US35513237

申请日：2021-06-24

申请人： Myoridge Co. Ltd.

设计人： Itsunari Minami

摘要： 1. Culture vessel
1.1 : is a front elevation view of an embodiment of a culture vessel having caps
1.2 : is a back elevation view of thereof
1.3 : is a top plan view thereof
1.4 : is a bottom plan view thereof
1.5 : is a right elevation view thereof
1.6 : is a left elevation view thereof
1.7: is a cross-sectional view thereof
1.8 : is a perspective top, front view thereof
1.9 : is a front elevation view of another embodiment of a culture vessel, without caps
1.10 : is a back elevation view of thereof
1.11 : is a top plan view thereof
1.12 : is a bottom plan view thereof
1.13 : is a right elevation view thereof
1.14 : is a left elevation view thereof
1.15 : is a cross-sectional view thereof
1.16 : is a perspective top, front view thereof

公开(公告)号：US20210363474A1

公开(公告)日：2021-11-25

申请号：US17251112

申请日：2019-07-04

申请人： MYORIDGE CO. LTD.

发明人： Itsunari Minami ,  Shinichi Sueta ,  Toyomi Nakamura ,  Saori Fujikawa ,  Maiko Minatohara

IPC分类号： C12M3/00 ,  C12M3/04 ,  C12M1/42 ,  C12N5/077

摘要： A method for producing a cell sheet, the method including subjecting animal cells dispersed in a culture medium to a suspension culture while gathering the animal cells toward the center of the culture medium, to form a cell sheet floating in the culture medium.