公开(公告)号：US20070104696A1

公开(公告)日：2007-05-10

申请号：US10574167

申请日：2004-10-07

Applicant: Thomas Bush ,  Ronald Faris ,  Jin Liu

Inventor： Thomas Bush ,  Ronald Faris ,  Jin Liu

IPC: A61K48/00 ,  C07H21/04 ,  C12P21/06 ,  C12N5/08 ,  C07K14/61 ,  C07K14/75

CPC classification number: C12N5/067 ,  A61K38/00 ,  C07K14/47 ,  C12N2503/02 ,  C12N2510/02 ,  C12N2510/04 ,  G01N33/5067 ,  G01N2333/90245 ,  Y02A50/467

Abstract: This invention relates to the production of proteins, including therapeutic plasma proteins, by virally-immortalized hepatocyte cell lines and by other eukaryotic cells, as well as to the use of such proteins for screening and therapy, as well as to nucleic acids, vectors, and transformed or transfected cells that carry the genetic information for proteins.

Abstract translation: 本发明涉及通过病毒永生化肝细胞细胞系和其它真核细胞产生蛋白质，包括治疗性血浆蛋白质，以及这些蛋白质用于筛选和治疗以及核酸，载体， 以及携带蛋白质遗传信息的转化或转染细胞。

公开(公告)号：US07566567B2

公开(公告)日：2009-07-28

申请号：US10574163

申请日：2004-10-07

Applicant: Jin Liu ,  Ronald A. Faris

Inventor： Jin Liu ,  Ronald A. Faris

IPC: C12N5/16 ,  C12N5/22

CPC classification number: C12N5/067 ,  A61K38/00 ,  C07K14/47 ,  C12N2503/02 ,  C12N2510/02 ,  C12N2510/04 ,  G01N33/5067 ,  G01N2333/90245 ,  Y02A50/467

Abstract: This invention relates to virally-immortalized hepatocyte cell lines, which are derived from a normal primary human liver cell, have the ability to proliferate in a serum-free media, are nontumorigenic, and produce proteins. These cell lines can be used for toxicity testing of potential therapeutic drugs and chemical entities. The cell lines may also be used for the production of therapeutic plasma proteins.

Abstract translation: 本发明涉及源自正常原代人肝细胞的病毒永生化肝细胞细胞系，具有在无血清培养基中增殖的能力，是非致癌性的，并且产生蛋白质。 这些细胞系可用于潜在的治疗药物和化学实体的毒性测试。 细胞系也可用于生产治疗性血浆蛋白。

公开(公告)号：US20070004039A1

公开(公告)日：2007-01-04

申请号：US10574163

申请日：2004-10-07

Applicant: Jin Liu ,  Ronald Farie

Inventor： Jin Liu ,  Ronald Farie

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC classification number: C12N5/067 ,  A61K38/00 ,  C07K14/47 ,  C12N2503/02 ,  C12N2510/02 ,  C12N2510/04 ,  G01N33/5067 ,  G01N2333/90245 ,  Y02A50/467

Abstract: This invention relates to virally-immortalized hepatocyte cell lines, which are derived from a normal primary human liver cell, have the ability to proliferate in a serum-free media, are nontumorigenic, and produce proteins. These cell lines can be used for toxicity testing of potential therapeutic drugs and chemical entities. The cell lines may also be used for the production of therapeutic plasma proteins.

Abstract translation: 本发明涉及源自正常原代人肝细胞的病毒永生化肝细胞细胞系，具有在无血清培养基中增殖的能力，是非致癌性的，并且产生蛋白质。 这些细胞系可用于潜在的治疗药物和化学实体的毒性测试。 细胞系也可用于生产治疗性血浆蛋白。