公开(公告)号：US20240209306A1

公开(公告)日：2024-06-27

申请号：US18287287

申请日：2022-12-21

申请人： POW Genetic Solutions, Inc.

发明人： Shannon HALL ,  Ouwei WANG ,  Brian LEE ,  Charles EMRICH

IPC分类号： C12M1/36 ,  C12M1/00 ,  C12M1/34 ,  C12N1/20

CPC分类号： C12M41/48 ,  C12M27/20 ,  C12M29/06 ,  C12M41/12 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34 ,  C12N1/20

摘要： Methods and systems for iteratively improving culture condition for at least one culture output in a culture process. The methods comprise: a) providing a continuous culture of cells in a bioreactor, wherein the cells are cultured under culture conditions that comprise a set of culture parameters. and wherein the culture of cells produces at least one culture output at a first measure: b) while maintaining the cells in continuous culture, iteratively testing different culture conditions by: (i) selecting a new culture condition comprising a new set of culture parameters predicted to improve the measure of the culture output: (ii) perturbing the cells with the new culture conditions: and (iii) determining a new measure of the culture output: wherein testing is iterated to produce a plurality of improvements in the measure of culture output. The iterative process can be performed by an automated artificial intelligence.