公开(公告)号：US20140080200A1

公开(公告)日：2014-03-20

申请号：US13516740

申请日：2010-12-15

申请人： Adam Charlton ,  Mark Napoli ,  Paul Smrdelj ,  Anthony Stowers ,  Vicky Pirzas ,  Magnus Schröder ,  Ian Walker

发明人： Adam Charlton ,  Mark Napoli ,  Paul Smrdelj ,  Anthony Stowers ,  Vicky Pirzas ,  Magnus Schröder ,  Ian Walker

IPC分类号： C07K1/18 ,  C07K16/00 ,  C12N9/50

CPC分类号： C07K1/18 ,  C07K1/22 ,  C07K16/00 ,  C12N9/6437 ,  C12N9/644 ,  C12Y304/21021 ,  C12Y304/21022

摘要： The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an ion-exchange matrix relative to the amount of one or more impurities bound to the ion-exchange matrix. This effect is achieved by adding a chemical compound in the process which by also binding to the ion-exchange matrix due to a change that is opposite to the change of the ion-exchange matrix, reduces the binding of impurities more than the binding of the polypeptide of interest.

摘要翻译： 本发明涉及通过相对于结合到离子交换基质的一种或多种杂质的量增加与离子交换基质结合的目标多肽的量来纯化目的多肽的改进方法。 通过在与离子交换基质的变化相反的离子交换基质的过程中加入化学化合物，可以实现这种效果，减少了杂质的结合， 感兴趣的多肽