公开(公告)号：US5922581A

公开(公告)日：1999-07-13

申请号：US833411

申请日：1997-04-04

申请人： Tatsuo Hoshino ,  Akifumi Noro ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Akifumi Noro ,  Masaaki Tazoe

IPC分类号： C12N1/20 ,  C12P17/18 ,  C12R1/01 ,  C12N1/00 ,  C12P17/10

CPC分类号： C12P17/186 ,  Y10S435/822

摘要： A process for producing d-biotin comprises cultivating a microorganism of the genus Kurthia and which is resistant to biotin antimetabolites and capable of producing d-biotin in a medium under aerobic conditions, and separating the resulting d-biotin from the fermentation broth. The cultivation medium suitably contains an assimilable carbon source, a digestible nitrogen source, inorganic salts and other nutrients necessary for the growth of the microorganism at a pH of about 5-9, temperature of about 10-40.degree. C. and for a duration of about 1-10 days. The preferred microorganism are Kurthia sp. 538-KA26, 538-17H4, 538-51F9 and 538-2A 13 (DSM No. 10609, 10608, 10610 and 10607, respectively), which are also new, and as such represent a further aspect of the present invention. The so-produced d-biotin is one of the essential vitamins for the nutrition of animals, plants and microorganisms, and is important as a medicine or food additive. It is produced in particularly high yield by the process of the present invention.

摘要翻译： 一种生产d-生物素的方法包括培养Kurthia属的微生物，其对生物素抗代谢物具有抗性，并且能够在需氧条件下在培养基中产生d-生物素，并将所得的d-生物素与发酵液分离。 栽培培养基适当地含有可吸收的碳源，可消化的氮源，无机盐和微生物生长所需的其它营养物，pH约为5-9，温度约为10-40℃，持续时间为 约1-10天。 优选的微生物是Kurthia sp。 538-KA26,538-17H4,538-51F9和538-2A 13（分别为DSM10609,10608,101010和10607），它们也是新的，因此代表本发明的另一方面。 如此生产的d生物素是动物，植物和微生物营养的必需维生素之一，并且作为药物或食品添加剂是重要的。 通过本发明的方法产生的产率特别高。