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    Vitamin C from sorbosone
    3.
    发明授权
    Vitamin C from sorbosone 失效
    维生素C来自sorbosone

    公开(公告)号：US07544494B2

    公开(公告)日：2009-06-09

    申请号：US10528885

    申请日：2003-09-22

    申请人： Tatsuo Hoshino ,  Taro Miyazaki ,  Teruhide Sugisawa

    发明人： Tatsuo Hoshino ,  Taro Miyazaki ,  Teruhide Sugisawa

    IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N9/06

    CPC分类号： C12P17/04

    摘要： The present invention relates to a process for the production of vitamin C from L sorbosone using an aldehyde dehydrogenase which is isolated from Gluconobacter oxydans DSM 4025 (FERM BP-3812), said enzyme having the following physicochemical properties: (a) molecular weight of 150,000±6,000 Da or 230,000±9,000 Da (consisting of 2 or 3 homologous subunits, each subunit having a molecular weight of 75,000±3,000 Da); (b) substrate specificity as active on aldehyde compounds; (c) Cofactors are pyrroloquinoline quinone and heme c; (d) Optimum pH between 6.4 and 8.2 for vitamin C production from L-sorbosone; and (e) as inhibitors Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and ethylendiamine tetraacetic acid. The process is performed in the presence of a suitable electron acceptor and the vitamin C isolated from the reaction mixture.

    摘要翻译： 本发明涉及使用从脱氧葡萄糖酸杆菌DSM 4025（FERM BP-3812）中分离的醛脱氢酶从L山梨糖酮生产维生素C的方法，所述酶具有以下物理化学性质：（a）分子量为15万 ±6,000Da或230,000±9,000Da（由2或3个同源亚基组成，每个亚基具有75,000±3,000Da的分子量）; （b）对醛化合物具有活性的底物特异性; （c）辅因子是吡咯并喹啉醌和血红素c; （d）从L-山梨糖酮生产维生素C的最佳pH在6.4和8.2之间; 和（e）作为抑制剂Co2 +，Cu2 +，Fe2 +，Ni2 +，Zn2 +，一碘乙酸酯和乙二胺四乙酸。 该方法在合适的电子受体和从反应混合物中分离的维生素C的存在下进行。

    Microbial production of vitamin C
    5.
    发明授权
    Microbial production of vitamin C 有权
    微生物生产维生素C

    公开(公告)号：US07341854B2

    公开(公告)日：2008-03-11

    申请号：US10528893

    申请日：2003-09-22

    申请人： Tatsuo Hoshino ,  Teruhide Sugisawa

    发明人： Tatsuo Hoshino ,  Teruhide Sugisawa

    IPC分类号： C12P17/04

    CPC分类号： C12P7/60 ,  C12P17/04

    摘要： The present invention provides a process for the production of vitamin C from different substrates like D-sorbitol, L-sorbose, L-sorbosone or L-gulose using a microorganism selected from the group consisting of Gluconobacter oxydans DMS 4025 (FERM BP-3812), a microorganism belonging to the genus Gluconobacter and having identifying characteristics of G. oxydans DSM 4025 (FERM BP-3812) and mutants thereof.

    摘要翻译： 本发明提供使用选自氧化葡糖杆菌DMS 4025（FERM BP-3812）的微生物从不同底物如D-山梨醇，L-山梨糖，L-山梨糖酮或L-古洛糖生产维生素C的方法， 属于葡萄糖杆菌属的微生物，具有G.oxydans DSM 4025（FERM BP-3812）的鉴定特征及其突变体。

    Aldehyde dehydrogenase gene
    6.
    发明授权
    Aldehyde dehydrogenase gene 有权
    醛脱氢酶基因

    公开(公告)号：US07135315B2

    公开(公告)日：2006-11-14

    申请号：US10528675

    申请日：2003-09-22

    申请人： Tatsuo Hoshino ,  Taro Miyazaki ,  Teruhide Sugisawa

    发明人： Tatsuo Hoshino ,  Taro Miyazaki ,  Teruhide Sugisawa

    IPC分类号： C12P7/40 ,  C12P7/60 ,  C12N9/04 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12N9/0006 ,  C12P7/60

    摘要： The present invention relates to a novel DNA which encodes aldehyde dehydrogenase (SNDH), an expression vector containing the DNA and recombinant organisms containing said DNA. Furthermore, the present invention concerns a process for producing recombinant aldehyde dehydrogenase protein and a process for producing L-ascorbic acid (vitamin C) and/or 2-keto-L-gulonic acid (2-KGA) from L-sorbosone by using the recombinant aldehyde dehydrogenase protein or recombinant organisms containing the expression vector. Also provided is a process for the production of 2KGA with a microorganism in which the gene encoding said aldehyde dehydrogenase is disrupted.

    摘要翻译： 本发明涉及编码醛脱氢酶（SNDH）的新型DNA，含有DNA的表达载体和含有所述DNA的重组生物。 此外，本发明涉及一种生产重组醛脱氢酶蛋白的方法和由L-山梨糖酮生产L-抗坏血酸（维生素C）和/或2-酮-L-古洛糖酸（2-KGA）的方法， 重组醛脱氢酶蛋白或含有表达载体的重组生物体。 还提供了用编码所述醛脱氢酶的基因被破坏的微生物生产2KGA的方法。

    Production of l-aldonolactone
    7.
    发明申请
    Production of l-aldonolactone 审中-公开
    L-己内酯的生产

    公开(公告)号：US20060166339A1

    公开(公告)日：2006-07-27

    申请号：US10528899

    申请日：2003-09-18

    申请人： Tatsuo Hoshino ,  Masako Shinjoh

    发明人： Tatsuo Hoshino ,  Masako Shinjoh

    IPC分类号： C12P17/06 ,  C12P17/04

    CPC分类号： C12P17/04 ,  C12P7/58

    摘要： The present invention provides a process for the production of L-aldonolactone from L-aldohexose, especially for producing L-gulono-1,4-lactone or L-gulonic acid from L-gulose and producing L-galactono-1,4-lactone or L-galactonic acid from L-galactose by a microorganism belonging to the genus Pseudomonas or the genus Gluconobacter.

    摘要翻译： 本发明提供了L- L-己内酯生产L-阿尔多内酯的方法，特别是用于从L-古洛糖生产L-古洛酮-1,4-内酯或L-古洛糖酸并生产L-半乳糖-1,4-内酯 或L-半乳糖酸，其由属于假单胞菌属或葡萄糖酸杆菌属的微生物由L-半乳糖得到。

    Alcohol/aldehyde dehydrogenase
    9.
    发明授权
    Alcohol/aldehyde dehydrogenase 有权
    醇/醛脱氢酶

    公开(公告)号：US06730503B1

    公开(公告)日：2004-05-04

    申请号：US09470667

    申请日：1999-12-22

    申请人： Akira Asakura ,  Tatsuo Hoshino ,  Setsuko Ojima ,  Masako Shinjoh ,  Noribumi Tomiyama

    发明人： Akira Asakura ,  Tatsuo Hoshino ,  Setsuko Ojima ,  Masako Shinjoh ,  Noribumi Tomiyama

    IPC分类号： C12P740

    CPC分类号： C12N9/0006 ,  C12P7/24 ,  C12P7/26 ,  C12P7/40 ,  C12P7/60 ,  C12P17/00

    摘要： The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or mom recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided. Furthermore the recombinant enzymes having alcohol and aldehyde dehydrogenase activity are used for obtaining aldehydes, ketones or carboxylic acids, and specifically, 2-keto-L-gulonic acid an intermediate for the production of L-ascorbic acid (vitamin C).

    摘要翻译： 本发明涉及具有醇和醛脱氢酶活性的重组酶，其包含一种或多种选自SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO： NO 8和作为SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO 8鉴定的至少两个以下氨基酸序列和上述多肽的功能衍生物的嵌合组合的嵌合重组多肽 其含有一个或多个氨基酸残基的添加，插入，缺失和/或取代，其中所述酶多肽具有所述醇和醛脱氢酶活性。 提供了编码重组多肽的DNA分子，包含这种DNA分子的载体，由这些载体转化的宿主细胞和用于产生这种重组酶的方法。 此外，具有醇和醛脱氢酶活性的重组酶用于获得醛，酮或羧酸，特别是2-酮-L-古洛糖酸，用于生产L-抗坏血酸（维生素C）的中间体。