公开(公告)号：US20080295259A1

公开(公告)日：2008-12-04

申请号：US12034793

申请日：2008-02-21

申请人： Shigeru UEDA ,  Takeshi MATSUOKA ,  Akihiko KAN

发明人： Shigeru UEDA ,  Takeshi MATSUOKA ,  Akihiko KAN

IPC分类号： C09B67/00

CPC分类号： C12Q1/28 ,  C09B67/0077

摘要： Provided are a method of stabilizing a leuco dye storable as a liquid for a long time, a method of reducing nonspecific color development thereof at a time of a color development reaction, and a stable liquid reagent composition using the methods. The inventors of the present invention found that coexistence of the leuco dye with a specific reducing agent resulted in suppression of self color development and its remarkably improved stability in a solution, and that when a color development reaction of the leuco dye with hydrogen peroxide was performed, coexistence of the leuco dye, in a reaction solution, with another dye which had an absorption spectrum not influencing a measurement wavelength of the leuco dye and did not react with hydrogen peroxide suppressed nonspecific color development and lowered a reagent blank value, which was applied to an analytical reagent.

摘要翻译： 提供一种稳定长时间保存为液体的无色染料的方法，在显色反应时减少其非特异性显色的方法和使用该方法的稳定的液体试剂组合物。 本发明人发现，无色染料与特定的还原剂共存导致自色显影的抑制和其在溶液中的显着提高的稳定性，并且当进行无色染料与过氧化氢的显色反应时 在无色染料的反应溶液中与另一种不影响无色染料的测定波长的吸收光谱并且不与过氧化氢反应的染料共存抑制非特异性显色，降低试剂空白值 分析试剂。

公开(公告)号：US08268017B2

公开(公告)日：2012-09-18

申请号：US12034793

申请日：2008-02-21

申请人： Shigeru Ueda ,  Takeshi Matsuoka ,  Akihiko Kan

发明人： Shigeru Ueda ,  Takeshi Matsuoka ,  Akihiko Kan

IPC分类号： C09B67/00 ,  D06P5/00 ,  G01N33/72 ,  G03C8/00 ,  C08B37/16 ,  C12N9/00 ,  C12Q1/00 ,  C12Q1/37 ,  C12Q1/26

CPC分类号： C12Q1/28 ,  C09B67/0077

摘要： Provided are a method of stabilizing a leuco dye storable as a liquid for a long time, a method of reducing nonspecific color development thereof at a time of a color development reaction, and a stable liquid reagent composition using the methods. The inventors of the present invention found that coexistence of the leuco dye with a specific reducing agent resulted in suppression of self color development and its remarkably improved stability in a solution, and that when a color development reaction of the leuco dye with hydrogen peroxide was performed, coexistence of the leuco dye, in a reaction solution, with another dye which had an absorption spectrum not influencing a measurement wavelength of the leuco dye and did not react with hydrogen peroxide suppressed nonspecific color development and lowered a reagent blank value, which was applied to an analytical reagent.

摘要翻译： 提供一种稳定长时间保存为液体的无色染料的方法，在显色反应时减少其非特异性显色的方法和使用该方法的稳定的液体试剂组合物。 本发明人发现，无色染料与特定的还原剂共存导致自色显影的抑制和其在溶液中的显着提高的稳定性，并且当进行无色染料与过氧化氢的显色反应时 在无色染料的反应溶液中与另一种不影响无色染料的测定波长的吸收光谱并且不与过氧化氢反应的染料共存抑制非特异性显色，降低试剂空白值 分析试剂。