公开(公告)号：US20110294129A1

公开(公告)日：2011-12-01

申请号：US13131174

申请日：2010-01-04

申请人： Ali Laayoun ,  Alain Troesch

发明人： Ali Laayoun ,  Alain Troesch

IPC分类号： C12P19/34 ,  C12N9/12 ,  C12Q1/68

CPC分类号： C12Q1/6848 ,  C12Q2523/125

摘要： A method of amplification and/or of detection for removing contaminants in a liquid biological sample containing nucleic acids of interest to amplify, which includes treating the biological sample chemically or enzymatically to convert one type of base of the nucleic acids of interest to another type of base; adding amplification primers, intended for specifically amplifying said converted nucleic acids of interest, each primer being constituted of three different types of bases; adding to the biological sample, after these treatments, amplification reagents the primers previously synthesized; and placing the solution and the reagents in conditions permitting amplification and/or detection of the converted nucleic acids.

摘要翻译： 一种用于去除含有目的核酸的液体生物样品中的污染物的扩增和/或检测方法，用于扩增，其包括以化学或酶学方式处理生物样品以将目的核酸的一种碱基转化为另一种类型的 基础; 加入用于特异性扩增所述转化的目的核酸的扩增引物，每个引物由三种不同类型的碱基组成; 加入生物样品后，经过这些处理，扩增试剂是先前合成的引物; 并将溶液和试剂置于允许扩增和/或检测转化的核酸的条件下。

公开(公告)号：US09365894B2

公开(公告)日：2016-06-14

申请号：US13131174

申请日：2010-01-04

申请人： Ali Laayoun ,  Alain Troesch

发明人： Ali Laayoun ,  Alain Troesch

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6848 ,  C12Q2523/125

摘要： A method of amplifying a nucleic acid of interest comprises (i) treating a biological sample chemically or enzymatically to permit conversion of one type of nucleic acid base to another type of base, (ii) purifying the treated biological sample before adding amplification primers and amplification reagents to the biological sample, (iii) adding the amplification primers and amplification reagents to the biological sample, each primer being constituted of three different types of bases and being specific to a converted nucleic acid of interest or to a nucleic acid that is complementary to the converted nucleic acid of interest, and (iv) amplifying the converted nucleic acid of interest provided that the nucleic acid of interest was present in the biological sample. In the method, amplification of contaminating nucleic acids is avoided by converting the one type of nucleic acid base to another type of base prior to adding the amplification reagents to the biological sample.

摘要翻译： 扩增目的核酸的方法包括（i）以化学或酶学方式处理生物样品以允许将一种类型的核酸碱基转化为另一种类型的碱，（ii）在加入扩增引物和扩增前纯化经处理的生物样品 生物样品的试剂，（iii）将扩增引物和扩增试剂加入到生物样品中，每个引物由三种不同类型的碱基构成，并且对转化的目的核酸具有特异性， 转化的目标核酸，以及（iv）扩增所转化的转化的核酸，只要感兴趣的核酸存在于生物样品中即可。 在该方法中，通过在向生物样品中加入扩增试剂之前将一种类型的核酸碱基转化为另一种碱基来避免污染性核酸的扩增。

公开(公告)号：US07659059B2

公开(公告)日：2010-02-09

申请号：US10560433

申请日：2004-07-08

申请人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

发明人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689

摘要： The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps: A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.

摘要翻译： 本发明涉及一种用于检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤：A.提​​取葡萄球菌属细菌的核酸物质，B.核酸的至少一个靶序列 使用至少一个包含SEQ ID No.1的至少10个核苷酸基序的扩增引物和/或至少一个包含SEQ ID No.2的至少10个核苷酸基序的扩增引物扩增葡萄球菌属细菌的酸性物质， 以获得靶序列的扩增子，C.通过检测所述扩增子来确定葡萄球菌属细菌的存在。

公开(公告)号：US20070292846A1

公开(公告)日：2007-12-20

申请号：US10560433

申请日：2004-07-08

申请人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

发明人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689

摘要： The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps: A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.

摘要翻译： 本发明涉及一种用于检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤：A.提​​取葡萄球菌属细菌的核酸物质，B.核酸的至少一个靶序列 使用至少一个包含SEQ ID No.1的至少10个核苷酸基序的扩增引物和/或至少一个包含SEQ ID No.2的至少10个核苷酸基序的扩增引物扩增葡萄球菌属细菌的酸性物质， 以获得靶序列的扩增子，C.通过检测所述扩增子来确定葡萄球菌属细菌的存在。