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1.发明申请Method for controlling the microbiological quality of an aqueous medium and kit therefor 审中-公开用于控制水性介质的微生物质量的方法及其试剂盒公开(公告)号：US20080171314A1
公开(公告)日：2008-07-17
申请号：US12010716
申请日：2008-01-29
申请人： Patricia Renaud , Emmanuelle Guillot , Claude Mabilat , Carole Vachon , Bruno Lacroix , Guy Vernet , Marie-Astrid Armand , Philippe Laffaire
发明人： Patricia Renaud , Emmanuelle Guillot , Claude Mabilat , Carole Vachon , Bruno Lacroix , Guy Vernet , Marie-Astrid Armand , Philippe Laffaire
CPC分类号： C12Q1/701 , C12Q1/689 , C12Q1/6893
摘要： Disclosed is a method for controlling the microbiological quality of an environmental aqueous medium, suspected of containing various micro-organisms, having the following steps: selecting a reference set, having at least three micro-organisms, representing jointly or separately a microbiological quality level; providing a microbiological detection kit, having at least three probes specifically and respectively identifying said three micro-organisms; after treating the medium to be analyzed, contacting said micro-organisms, or any fraction thereof derived from the medium to be analyzed therefrom, with said detection kit, whereby a multiple determination of the micro-organisms is carried out, the determination representing the microbiological quality level of the medium.
摘要翻译： 公开了一种用于控制怀疑含有各种微生物的环境水性介质的微生物质量的方法，具有以下步骤：选择具有至少三种微生物的参考文献，共同或分别表示微生物质量水平; 提供微生物检测试剂盒，具有特异性和分别鉴定所述三种微生物的至少三种探针; 在处理要分析的培养基之后，将所述微生物或其衍生自待分析培养基的任何部分与所述检测试剂盒接触，由此进行微生物的多重测定，所述微生物 质量水平的介质。
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公开(公告)号：US20060263784A1
公开(公告)日：2006-11-23
申请号：US10572905
申请日：2004-10-01
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/118
摘要： The present invention relates to a method for neuroblastoma prognosis in a patient suffering from neuroblastoma, characterized in that it comprises the following steps: a. biological material is extracted from a biological sample taken from the patient, b. the biological material is brought into contact with at least one specific reagent chosen from the reagents specific for the target genes exhibiting a nucleic acid sequence having any one of SEQ ID Nos. 1 to 37, it being understood that, when the target gene exhibits a nucleic acid sequence having one of SEQ ID Nos. 11, 17 or 37, the biological material is brought into contact with at least two specific reagents chosen from the reagents specific for the target genes exhibiting a nucleic acid sequence having any one of SEQ ID Nos. 1 to 37, c. the expression of at least one of said target genes is determined, it being understood that, when the target gene exhibits a nucleic acid sequence having one of SEQ ID Nos. 11, 17 or 37, the expression of at least two of said target genes is determined.
摘要翻译： 本发明涉及神经母细胞瘤患者神经母细胞瘤预后的方法，其特征在于包括以下步骤：a。 生物材料从取自患者的生物样品中提取，b。 使生物材料与选自对具有SEQ ID No.1至37中任一项所示的核酸序列的靶基因特异性的试剂中的至少一种特异性试剂接触，应理解，当靶基因表现出 具有SEQ ID No.11,17或37之一的核酸序列，使生物材料与至少两种特异性试剂接触，所述试剂选自对具有SEQ ID NO：11,17或37中任一项所示的核酸序列的靶基因特异性的试剂 1至37，c。 确定至少一个所述靶基因的表达，应当理解，当靶基因表现出具有SEQ ID No.11,17或37之一的核酸序列时，至少两个所述靶基因的表达 决心，决意，决定。
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公开(公告)号：US20070027308A1
公开(公告)日：2007-02-01
申请号：US10631441
申请日：2003-07-30
CPC分类号： C07K14/47
摘要： The sequences of 5′ ESTs derived from mRNAs encoding secreted proteins are disclosed. The 5′ ESTs may be to obtain cDNAs and genomic DNAs corresponding to the 5′ ESTs. The 5′ ESTs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. Upstream regulatory sequences may also be otained using the 5′ ESTs. The 5′ ESTs may also be used to design expression vectors and secretion vectors.
摘要翻译： 公开了从编码分泌蛋白的mRNA衍生的5'EST的序列。 5'EST可能是获得与5'EST相对应的cDNA和基因组DNA。 5'EST也可用于诊断，法医，基因治疗和染色体测绘程序。 也可以使用5'EST来获得上游调控序列。 5'EST也可用于设计表达载体和分泌载体。
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4.发明授权公开(公告)号：US07659059B2
公开(公告)日：2010-02-09
申请号：US10560433
申请日：2004-07-08
申请人： Alain Troesch , Bruno Lacroix , Corinne Jay
发明人： Alain Troesch , Bruno Lacroix , Corinne Jay
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps: A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.
摘要翻译： 本发明涉及一种用于检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤：A.提取葡萄球菌属细菌的核酸物质，B.核酸的至少一个靶序列 使用至少一个包含SEQ ID No.1的至少10个核苷酸基序的扩增引物和/或至少一个包含SEQ ID No.2的至少10个核苷酸基序的扩增引物扩增葡萄球菌属细菌的酸性物质， 以获得靶序列的扩增子，C.通过检测所述扩增子来确定葡萄球菌属细菌的存在。
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5.发明申请公开(公告)号：US20070292846A1
公开(公告)日：2007-12-20
申请号：US10560433
申请日：2004-07-08
申请人： Alain Troesch , Bruno Lacroix , Corinne Jay
发明人： Alain Troesch , Bruno Lacroix , Corinne Jay
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps: A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.
摘要翻译： 本发明涉及一种用于检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤：A.提取葡萄球菌属细菌的核酸物质，B.核酸的至少一个靶序列 使用至少一个包含SEQ ID No.1的至少10个核苷酸基序的扩增引物和/或至少一个包含SEQ ID No.2的至少10个核苷酸基序的扩增引物扩增葡萄球菌属细菌的酸性物质， 以获得靶序列的扩增子，C.通过检测所述扩增子来确定葡萄球菌属细菌的存在。
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6.发明申请Method for the detection and/or identification of the original animal species in animal matter contained in a sample 审中-公开用于检测和/或鉴定样品中包含的动物物质中的原始动物物种的方法公开(公告)号：US20050069890A1
公开(公告)日：2005-03-31
申请号：US10500646
申请日：2003-01-10
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68 , C12Q1/6876
CPC分类号： C12Q1/6876 , C12Q2600/16
摘要： Disclosed is a method for detecting or identifying the original animal species in a sample likely to contain an ingredient obtained at least from said species. The inventive method is characterized by the following steps: a) a nuclear fraction is obtained from said sample; b) at least one reactant which is specific to the animal species is provided and selected among a group formed by: - the reference sequences SEQ ID numbers 1 to 232, 242 to 261; - the sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; - the sequences homologous to each of the sequences SEQ ID numbers 1 to 232, 242 to 261 and sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; c) the nuclear fraction and said reactant are reacted with each other; and d) any signal or information resulting from the specific reaction between said reactant and the nuclear fraction is detected, whereby it can be established if said sample contains said original animal species.
摘要翻译： 公开了一种用于检测或鉴定可能含有至少从所述物种获得的成分的样品中的原始动物物种的方法。 本发明的方法的特征在于以下步骤：a）从所述样品获得核级分; b）至少一种对动物物种具有特异性的反应物，由选自下组形成的基团： - 参考序列SEQ ID号1至232,242至261; - 分别与每个序列SED ID号1至232,242至261互补的序列; - 与SEQ ID号1至232,242至261中的每一个序列同源的序列和分别与每个序列SED ID编号1至232,242至261互补的序列; c）核部分和所述反应物彼此反应; 并且d）检测由所述反应物和核部分之间的特异性反应产生的任何信号或信息，由此如果所述样品包含所述原始动物物种可以确定。
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公开(公告)号：US07393663B2
公开(公告)日：2008-07-01
申请号：US10631441
申请日：2003-07-30
CPC分类号： C07K14/47
摘要： The sequences of 5′ ESTs derived from mRNAs encoding secreted proteins are disclosed. The 5′ ESTs may be to obtain cDNAs and genomic DNAs corresponding to the 5′ ESTs. The 5′ ESTs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. Upstream regulatory sequences may also be obtained using the 5′ ESTs. The 5′ ESTs may also be used to design expression vectors and secretion vectors.
摘要翻译： 公开了从编码分泌蛋白的mRNA衍生的5'EST的序列。 5'EST可能是获得与5'EST相对应的cDNA和基因组DNA。 5'EST也可用于诊断，法医，基因治疗和染色体测绘程序。 也可以使用5'EST获得上游调控序列。 5'EST也可用于设计表达载体和分泌载体。
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8.发明申请公开(公告)号：US20060046246A1
公开(公告)日：2006-03-02
申请号：US10831286
申请日：2004-04-26
CPC分类号： C12Q1/689 , C12Q1/6837 , C12Q1/686 , G16B30/00
摘要： Materials and methods for identifying unique sites in bacterial 16S and 23S rDNA are provided, as well as specific unique sequences of 16S rDNA in select bacteria. The distinguishing moieties will enable rapid differentiation between families, genera, groups, species, strains, subspecies, and isolates of microorganisms. Such differentiation can be performed by using rapid screening kits in combination with in silico analysis for diagnostic, prognastic, epidemiologic, phylogenetic, and other purposes.
摘要翻译： 提供了在细菌16S和23S rDNA中识别独特位点的材料和方法，以及选择细菌中16S rDNA的特异性独特序列。 区别部分将能够快速区分家族，属，群体，物种，菌株，亚种和微生物分离物。 这种分化可以通过使用快速筛选试剂盒与用于诊断，预后，流行病学，系统发育和其它目的的计算机分析结合来进行。
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公开(公告)号：US06222029B1
公开(公告)日：2001-04-24
申请号：US08905223
申请日：1997-08-01
IPC分类号： C07H2104
CPC分类号： C07K14/47 , C07K2319/02
摘要： The sequences of 5′ ESTs derived from mRNAs encoding secreted proteins are disclosed. The 5′ ESTs may be to obtain cDNAs and genomic DNAs corresponding to the 5′ ESTs. The 5′ ESTs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. Upstream regulatory sequences may also be otained using the 5′ ESTs. The 5′ ESTs may also be used to design expression vectors and secretion vectors.
摘要翻译： 公开了从编码分泌蛋白的mRNA衍生的5'EST的序列。 5'EST可能是获得与5'EST相对应的cDNA和基因组DNA。 5'EST也可用于诊断，法医，基因治疗和染色体测绘程序。 也可以使用5'EST来获得上游调控序列。 5'EST也可用于设计表达载体和分泌载体。
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