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    MARKING REAGENTS BEARING DIAZO AND NITRO FUNCTIONS, METHODS FOR THE SYNTHESIS OF SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES
    1.
    发明申请
    MARKING REAGENTS BEARING DIAZO AND NITRO FUNCTIONS, METHODS FOR THE SYNTHESIS OF SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES 失效
    携带DIAZO和NITRO功能的标记试剂，合成这些试剂的方法和检测生物分子的方法

    公开(公告)号：US20100136538A1

    公开(公告)日：2010-06-03

    申请号：US12451502

    申请日：2008-06-09

    申请人： Alain Laurent ,  Ali Laayoun ,  Mitsuharu Kotera

    发明人： Alain Laurent ,  Ali Laayoun ,  Mitsuharu Kotera

    IPC分类号： C12Q1/68 ,  C07D495/04 ,  C07H21/00 ,  C12P19/34

    CPC分类号： C07D495/04 ,  Y10T436/13

    摘要： The present invention relates to a labeling reagent of formula: in which: R1 represents at least one detectable label, L and A are each a linker arm, n is an integer equal to 1, and u is an integer between 0 and 2. The present invention also describes a method of synthesizing said markers and also applications for the labeling of biological molecules, more particularly nucleic acids, with a labeling reagent bearing diazo and nitro functions. The invention is particularly suitable for use in the diagnostics field.

    摘要翻译： 本发明涉及下式的标记试剂：其中：R1表示至少一个可检测标记，L和A各自为连接臂，n为等于1的整数，u为0至2之间的整数。 本发明还描述了一种合成所述标记的方法，以及用于用具有重氮和硝基功能的标记试剂标记生物分子，更特别是核酸的应用。 本发明特别适合用于诊断领域。

    Functionalization Processes and Reactants Used in Such Processes Using an Isatoic Anhydride or a Derivative Thereof, Biological Molecules Thus Treated and Kits
    2.
    发明申请
    Functionalization Processes and Reactants Used in Such Processes Using an Isatoic Anhydride or a Derivative Thereof, Biological Molecules Thus Treated and Kits 审中-公开
    官能化方法和反应物在这些方法中使用的盐酸酐或其衍生物，如此处理的生物分子和试剂盒

    公开(公告)号：US20130253179A1

    公开(公告)日：2013-09-26

    申请号：US13992049

    申请日：2011-12-05

    申请人： Arnaud Burr ,  Ali Laayoun ,  Alain Laurent

    发明人： Arnaud Burr ,  Ali Laayoun ,  Alain Laurent

    IPC分类号： C07H21/02

    CPC分类号： C07H21/02 ,  C07D413/12 ,  C07D413/14 ,  C07D495/04 ,  C12N15/111 ,  C12N2330/30 ,  C12Q1/68 ,  C12Q2563/179

    摘要： The present invention relates to a process of functionalising at least one ribonucleic acid (RNA) molecule which comprises the following steps: a) having at least: a binding molecule constituted by an isatoic anhydride or a derivative thereof, a group of interest, and a binding arm linking the binding molecule with the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group in: position 2′ of the ribose of one of the RNA nucleotides, and/or position(s) 2′ and/or 3′ of the ribose of the nucleotide at the 3′ terminal end of the RNA, and c) obtaining an anthranilate linking, via the binding arm, the RNA to the group of interest. The invention also relates to a functionalising reagent able to be used in such processes, a functionalised biological RNA molecule capable of being obtained by these processes and a kit for detecting a target RNA molecule comprising such a reagent.Said invention finds a preferential application in the field of in vitro diagnosis.

    摘要翻译： 本发明涉及功能化至少一种核糖核酸（RNA）分子的方法，该方法包括以下步骤：a）至少具有：由靛红酸酐或其衍生物，感兴趣的组和 将结合分子与感兴趣的基团连接的结合臂，b）使结合分子的酸酐官能团与至少一个羟基在一个RNA核苷酸的核糖的位置2'和/ 2'和/或3'，并且c）获得通过结合臂将RNA连接到感兴趣的组中的邻氨基苯甲酸酯。 本发明还涉及能够用于这些方法的功能化试剂，能够通过这些方法获得的官能化生物RNA分子和用于检测包含这种试剂的靶RNA分子的试剂盒。 所述发明在体外诊断领域中优先应用。

    Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules
    3.
    发明授权
    Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules 失效
    标记试剂，合成这些试剂的方法和检测生物分子的方法

    公开(公告)号：US07691635B2

    公开(公告)日：2010-04-06

    申请号：US10590973

    申请日：2005-03-24

    申请人： Ali Laayoun ,  Eloy Bernal-Mendez

    发明人： Ali Laayoun ,  Eloy Bernal-Mendez

    IPC分类号： G01N37/00 ,  G01N33/48 ,  C07D239/70 ,  A61K31/4188

    CPC分类号： C07D495/04 ,  Y10T436/13 ,  Y10T436/143333

    摘要： The present invention relates to a temperature-stable labeling reagent of formula (0): in which: R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers interlinked by at least one multimeric structure, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R3 and R4 represent, independently of one another: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR, —CO—NH—(CH2)3—(O—CH2—CH2)3—CH2—NH—R2, —CO—NH—(CH2)3—(O—CH2—CH2)4—CH2—NH—R2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, preferably 0 or 1, —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—, —Z— represents —NH—, —NHCO—, —CONH— or —O—, m is an integer between 1 and 10, preferably between 1 and 3, and p is an integer between 1 and 10, preferably between 1 and 3. The present invention also describes a method for the synthesis of said labels and also applications for the labeling of biological molecules, in particular of nucleic acids, with a labeling reagent bearing the diazomethyl function.The invention is particularly suitable for use in the field of diagnostics.

    摘要翻译： 本发明涉及式（O）的温度稳定的标记试剂：其中：R 1表示H或烷基，芳基或取代的芳基，R 2表示可检测标记或至少两个可检测标记，其与至少一个多聚体 结构，L是包含至少两个共价键的直链的连接臂，n是等于0或1的整数，R 3和R 4彼此独立地表示：H，NO 2，Cl，Br，F，I， R 2 - （L）n Y-X，OR，SR，NR 2，R，NHCOR，CONHR，COOR，-CO-NH-（CH 2）3 - （O-CH 2 -CH 2）3 -CH 2 -NH-R 2， CO-NH-（CH 2）3 - （O-CH 2 -CH 2）4 -CH 2 -NH-R 2，其中R =烷基或芳基，A是连接臂，其包含至少一个共价双键，使得重氮官能团与 芳环和u是0和2之间的整数，优选0或1，-Y-X-表示-CONH - ， - NHCO - ， - CH 2 O - ， - CH 2 S - ， - Z-表示-NH-，-NHCO - ， - CONH-或-O-，m是1 a之间的整数 nd 10，优选在1和3之间，p是1至10之间，优选1至3之间的整数。本发明还描述了合成所述标记的方法以及用于标记生物分子的应用，特别是 的核酸，具有带有重氮甲基功能的标记试剂。 本发明特别适用于诊断领域。

    Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
    5.
    发明授权
    Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby 有权
    用于标记核糖核酸的方法和由此获得的标记的RNA片段

    公开(公告)号：US06376179B1

    公开(公告)日：2002-04-23

    申请号：US09446156

    申请日：1999-12-17

    申请人： Ali Laayoun

    发明人： Ali Laayoun

    IPC分类号： C12Q168

    CPC分类号： C07H21/00

    摘要： The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a terminal phosphate of each fragment for further reaction, and labeling each fragment at the freed terminal phosphate which is located at the 3′ end and/or the 5′ end of each fragment of the RNA, and to the use of such RNA fragments, for example, in the field of medical diagnosis.

    摘要翻译： 本发明涉及用于标记合成或天然核糖核酸（RNA）的方法。 它还涉及RNA片段，其已经通过片段化RNA来标记以释放每个片段的末端磷酸酯用于进一步的反应，并且在位于3'末端和/或5'端的释放的磷酸末端标记每个片段， RNA的每个片段的末端，以及例如在医学诊断领域中使用这样的RNA片段。

    Methods and kits for avoiding amplification of contaminating nucleic acids
    6.
    发明授权
    Methods and kits for avoiding amplification of contaminating nucleic acids 有权
    用于避免污染性核酸扩增的方法和试剂盒

    公开(公告)号：US09365894B2

    公开(公告)日：2016-06-14

    申请号：US13131174

    申请日：2010-01-04

    申请人： Ali Laayoun ,  Alain Troesch

    发明人： Ali Laayoun ,  Alain Troesch

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6848 ,  C12Q2523/125

    摘要： A method of amplifying a nucleic acid of interest comprises (i) treating a biological sample chemically or enzymatically to permit conversion of one type of nucleic acid base to another type of base, (ii) purifying the treated biological sample before adding amplification primers and amplification reagents to the biological sample, (iii) adding the amplification primers and amplification reagents to the biological sample, each primer being constituted of three different types of bases and being specific to a converted nucleic acid of interest or to a nucleic acid that is complementary to the converted nucleic acid of interest, and (iv) amplifying the converted nucleic acid of interest provided that the nucleic acid of interest was present in the biological sample. In the method, amplification of contaminating nucleic acids is avoided by converting the one type of nucleic acid base to another type of base prior to adding the amplification reagents to the biological sample.

    摘要翻译： 扩增目的核酸的方法包括（i）以化学或酶学方式处理生物样品以允许将一种类型的核酸碱基转化为另一种类型的碱，（ii）在加入扩增引物和扩增前纯化经处理的生物样品 生物样品的试剂，（iii）将扩增引物和扩增试剂加入到生物样品中，每个引物由三种不同类型的碱基构成，并且对转化的目的核酸具有特异性， 转化的目标核酸，以及（iv）扩增所转化的转化的核酸，只要感兴趣的核酸存在于生物样品中即可。 在该方法中，通过在向生物样品中加入扩增试剂之前将一种类型的核酸碱基转化为另一种碱基来避免污染性核酸的扩增。

    Detection probe
    8.
    发明授权
    Detection probe 失效
    检测探头

    公开(公告)号：US08663923B2

    公开(公告)日：2014-03-04

    申请号：US12996457

    申请日：2009-07-03

    申请人： Ali Laayoun ,  Eloy Bernal Mendez

    发明人： Ali Laayoun ,  Eloy Bernal Mendez

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/703 ,  C12Q1/6818 ,  C12Q1/701 ,  C12Q2525/307 ,  C12Q2525/301

    摘要： A nucleotide probe permitting the detection of nucleic acids and constituted of a labeled nucleotide strand having three fragments: a first fragment having a first closing sequence, a second fragment, all or part of which has a recognition sequence for the molecular recognition of the target nucleic acid and a third fragment having a second closing sequence, and at least two markers, one of the ends of the strand of the detection probe being free of any marker, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, the closing sequences thus maintaining the probe in a conformation that cannot be detected in the absence of the target nucleic acid.

    摘要翻译： 允许检测核酸并由具有三个片段的标记的核苷酸链构成的核苷酸探针：具有第一封闭序列的第一片段，其全部或部分具有用于靶核酸的分子识别的识别序列的第二片段 酸和具有第二闭合序列的第三片段和至少两个标记，所述检测探针的链的末端之一没有任何标记，其中当两个封闭序列杂交在一起时，检测探针具有 完全圆形的形状，因此关闭序列因此将探针保持在不存在靶核酸的情况下不能被检测的构象。

    METHOD FOR DECONTAMINATING A SOLUTION WITH RESPECT TO UNWANTED NUCLEIC ACIDS
    9.
    发明申请
    METHOD FOR DECONTAMINATING A SOLUTION WITH RESPECT TO UNWANTED NUCLEIC ACIDS 审中-公开
    一种解决无污染核酸的溶液的方法

    公开(公告)号：US20100086976A1

    公开(公告)日：2010-04-08

    申请号：US12450020

    申请日：2008-03-28

    申请人： Glaucia Paranhos-Baccala ,  Alain Laurent ,  Ali Laayoun

    发明人： Glaucia Paranhos-Baccala ,  Alain Laurent ,  Ali Laayoun

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6848 ,  C12Q2523/107

    摘要： The present invention concerns a method for decontaminating a solution of any nucleic acid present in said solution, comprising the following steps: for a sufficient period, subjecting the solution to the action of at least one type of molecule having the property of degrading the nucleic acids by fragmentation, termed a fragmentation molecule, until said nucleic acids, termed contaminating nucleic acids, have been completely degraded; and stopping the activity of the fragmentation molecule. The invention also concerns the use of said treated solution.The preferred application of the invention is in the field of diagnostics.

    摘要翻译： 本发明涉及一种用于去除所述溶液中存在的任何核酸的溶液的方法，包括以下步骤：在足够的时间内，使溶液经受至少一种具有降解核酸性质的分子的作用 通过片段化，称为断裂分子，直到称为污染性核酸的所述核酸已经完全降解; 并停止破碎分子的活性。 本发明还涉及使用所述处理过的溶液。 本发明的优选应用在诊断领域。

    Process for Labeling and Purification of Nucleic Acids of Interest Present in a Biological Sample to be Treated in a Single Reaction Vessel
    10.
    发明申请
    Process for Labeling and Purification of Nucleic Acids of Interest Present in a Biological Sample to be Treated in a Single Reaction Vessel 审中-公开
    用于在单个反应容器中处理的生物样品中存在的感兴趣的核酸的标记和纯化方法

    公开(公告)号：US20080233632A1

    公开(公告)日：2008-09-25

    申请号：US11658028

    申请日：2005-07-21

    申请人： Ali Laayoun ,  Lionel Menou ,  Frederic Ginot

    发明人： Ali Laayoun ,  Lionel Menou ,  Frederic Ginot

    IPC分类号： C07H1/00

    CPC分类号： C07D333/00 ,  C07D233/32 ,  C07D495/04 ,  C07H1/08 ,  C07H21/00

    摘要： The present invention relates to a process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising: taking a single reaction vessel, introducing into the reaction vessel: the biological sample, at least one labeling reagent for nucleic acids, at least one solid support enabling the adsorption of said nucleic acids, any ingredient necessary for the labeling of the nucleic acids and/or for the immobilization of said nucleic acids on the support, incubating the contents of the reaction vessel, and isolating the nucleic acids thus labeled. The invention finds a preferred application in the diagnostics field.

    摘要翻译： 本发明涉及一种用于标记和纯化待处理的生物样品中感兴趣的核酸的方法，包括：取单个反应容器，将反应容器引入生物样品，至少一种用于核酸的标记试剂 酸，至少一种能够吸附所述核酸的固体支持物，用于标记核酸所必需的任何成分和/或将所述核酸固定在载体上，孵育反应容器的内容物，并分离 如此标记的核酸。 本发明在诊断领域中找到了优选的应用。