公开(公告)号：US10059745B2

公开(公告)日：2018-08-28

申请号：US13578556

申请日：2011-02-11

申请人： Alberto Brandariz Nunez ,  Rebeca Menaya Vargas ,  Francisco Javier Benavente Martinez ,  Jose Manuel Martinez Costas

发明人： Alberto Brandariz Nunez ,  Rebeca Menaya Vargas ,  Francisco Javier Benavente Martinez ,  Jose Manuel Martinez Costas

IPC分类号： A61K39/15 ,  C07K14/005 ,  A61K38/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2720/12222 ,  Y02A50/385

摘要： The invention is based on the identification of the minimum region of the avian Orthoreovirus muNS protein which is capable of forming inclusions as well as on the identification of specific regions of the muNS protein showing capacity to associate with the inclusions formed by muNS. The identification of said regions allows developing methods for purifying recombinant polypeptides as well as methods for detecting the interaction between two polypeptides of interest.

公开(公告)号：US20120301493A1

公开(公告)日：2012-11-29

申请号：US13578556

申请日：2011-02-11

申请人： Alberto Brandariz Nunez ,  Rebeca Menaya Vargas ,  Francisco Javier Benavente Martinez ,  Jose Manuel Martinez Costas

发明人： Alberto Brandariz Nunez ,  Rebeca Menaya Vargas ,  Francisco Javier Benavente Martinez ,  Jose Manuel Martinez Costas

IPC分类号： A61K39/15 ,  C12N15/46 ,  C07K19/00 ,  C12N15/62 ,  C12N1/21 ,  A61P37/04 ,  A61P31/04 ,  A61P31/10 ,  C12N5/10 ,  C12N1/19 ,  C07K14/14 ,  A61P31/14

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2720/12222 ,  Y02A50/385

摘要： The invention is based on the identification of the minimum region of the avian Orthoreovirus muNS protein which is capable of forming inclusions as well as on the identification of specific regions of the muNS protein showing capacity to associate with the inclusions formed by muNS. The identification of said regions allows developing methods for purifying recombinant polypeptides as well as methods for detecting the interaction between two polypeptides of interest.

摘要翻译： 本发明基于鉴定能够形成包涵体的禽类正交免疫球蛋白muNS蛋白的最小区域，以及鉴定显示与由muNS形成的夹杂物相关的能力的muNS蛋白质的特定区域。 所述区域的鉴定允许开发用于纯化重组多肽的方法以及用于检测两种感兴趣的多肽之间的相互作用的方法。