公开(公告)号：US5807735A

公开(公告)日：1998-09-15

申请号：US831400

申请日：1997-04-01

Applicant: Alexander A. Klibanov ,  Kim Lewis ,  Anthony Ferrante ,  Catherine L. Coyle ,  Gerben Zylstra ,  Michael S. P. Logan ,  Matthew J. Grossman

Inventor： Alexander A. Klibanov ,  Kim Lewis ,  Anthony Ferrante ,  Catherine L. Coyle ,  Gerben Zylstra ,  Michael S. P. Logan ,  Matthew J. Grossman

IPC: C12N9/02 ,  C12N9/08 ,  C12N15/52 ,  C12N15/53 ,  C12N15/70 ,  C07H21/04 ,  C12N9/04

CPC classification number: C12N9/0036 ,  C12N15/52 ,  C12N9/0065 ,  C12R1/19

Abstract: The present invention includes microorganisms that are resistant to non-aqueous solvents. In addition, the organisms of interest are able to grow and/or carry out various organic (e.g. hydrocarbon) transformations in non-aqueous/aqueous mixtures. The invention also includes a gene which encodes an enzyme, hydroperoxide reductase, which renders the host microorganism resistant to many organic solvents. The invention also includes the operon which includes the mutant gene and the ahpF gene which encodes an NAD(P)H dehydrogenase. The invention also includes a plasmid vehicle and a host microorganism containing these genes. The invention also includes cloning the genes encoding for solvent-resistance into other organisms rendering them solvent-resistant. In addition, the genes encoding for a specific organic or hydrocarbon transformation are placed into the solvent resistant microorganism.

Abstract translation: 本发明包括耐非水溶剂的微生物。 此外，感兴趣的生物体能够在非水/含水混合物中生长和/或进行各种有机（例如烃）转化。 本发明还包括编码酶，氢过氧化物还原酶的基因，其使得宿主微生物对许多有机溶剂具有抗性。 本发明还包括包含突变基因和编码NAD（P）H脱氢酶的ahpF基因的操纵子。 本发明还包括质粒载体和含有这些基因的宿主微生物。 本发明还包括将编码耐溶剂性的基因克隆到其具有耐溶剂性的其他生物体中。 此外，将编码特异性有机或烃转化的基因置于耐溶剂微生物中。