公开(公告)号：US20180312816A1

公开(公告)日：2018-11-01

申请号：US15977788

申请日：2018-05-11

申请人： President and Fellows of Harvard College

发明人： Jonathan Beckwith ,  Dana Boyd ,  Jessica Blazyk ,  Feras Hatahet

IPC分类号： C12N9/04

CPC分类号： C12N9/0006 ,  C07K14/245 ,  C07K14/705 ,  C12N9/0051 ,  C12N9/52 ,  C12N9/90 ,  C12P21/02 ,  C12R1/19 ,  C12Y101/04001 ,  G01N33/5041 ,  G01N2333/904

摘要： Provided herein are host cells or host cellular expression systems that express a membrane protein. Also, methods are provided that use such host cells or host cellular expression systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.

公开(公告)号：US20180245062A1

公开(公告)日：2018-08-30

申请号：US15748164

申请日：2016-07-29

申请人： CJ CHEILJEDANG CORPORATION

发明人： Sung Jae YANG ,  Yang Hee KIM ,  IL Hyang PARK ,  Young Mi LEE ,  Hyun Kug CHO ,  Seong Bo KIM ,  Seung Won PARK

IPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12N1/20 ,  C12P19/02 ,  C12P19/24 ,  C12R1/19 ,  C12Y501/02

摘要： A hexuronate C4-epimerase with improved conversion activity and a method for producing D-tagatose using the hexuronate C4-epimerase. The hexuronate C4-epimerase includes an amino acid sequence set forth in SEQ ID NO: 1, in which serine (S) at position 125, serine (S) at position 185, valine (V) at position 267, serine (S) at position 268, threonine (T) at position 272, tryptophan (W) at position 306, arginine (R) at position 386 and tyrosine (Y) at position 403 from an N-terminal of hexunorate C4-epimerase are mutated.

公开(公告)号：US10006063B2

公开(公告)日：2018-06-26

申请号：US14891776

申请日：2014-05-23

申请人： Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

发明人： Xueli Zhang ,  Xinna Zhu ,  Hongtao Xu ,  Zaigao Tan

IPC分类号： C12N1/20 ,  C12P7/46 ,  C12R1/19 ,  C12N15/52 ,  C12N9/02

CPC分类号： C12P7/46 ,  C12N1/20 ,  C12N9/0051 ,  C12N15/52 ,  C12R1/19 ,  C12Y108/01004

摘要： The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.

公开(公告)号：US20180171284A1

公开(公告)日：2018-06-21

申请号：US15893028

申请日：2018-02-09

申请人： Bobban Subhadra

发明人： Bobban Subhadra

IPC分类号： C12N1/20 ,  C12M1/12 ,  A61K9/00 ,  A61K35/74 ,  C12N7/00 ,  C12N1/04 ,  C12M1/34 ,  C12M1/00 ,  C12M3/00 ,  A61K35/00

CPC分类号： C12N1/20 ,  A61K9/0031 ,  A61K9/0053 ,  A61K35/74 ,  A61K2035/11 ,  C12M23/02 ,  C12M23/06 ,  C12M23/20 ,  C12M23/42 ,  C12M25/10 ,  C12M41/12 ,  C12M41/14 ,  C12M41/34 ,  C12N1/04 ,  C12N7/00 ,  C12N2795/00032 ,  C12N2795/00051 ,  C12R1/01 ,  C12R1/145 ,  C12R1/19 ,  C12R1/46 ,  G01N33/558 ,  Y02A50/463 ,  Y02A50/473 ,  Y02A50/475

摘要： One embodiment provides a commensal gut production platform for ex vivo production of human gut commensal microbiota. Another embodiment provides devices, systems and methods for ex vivo culturing of gut microflora in a system that mimics the human gut environment. The culturing of the commensal microbiota in the disclosed systems produces gut microbiota having defined characteristics and properties that can be exploited to treat various conditions in a subject.

公开(公告)号：US20180135007A1

公开(公告)日：2018-05-17

申请号：US14578494

申请日：2014-12-22

申请人： Nadia Adam

发明人： Nadia Adam

IPC分类号： C12N1/20 ,  C12R1/19 ,  C12N15/82 ,  A01N63/02

CPC分类号： C12N1/20 ,  A01H3/00 ,  A01N63/02 ,  C12N15/8261 ,  C12R1/01 ,  C12R1/19 ,  Y02P60/218 ,  A01N25/00

摘要： Endophytic microbial strains as biocatalysts isolated from fresh plant samples, compositions, and methods of use thereof to enhance the growth and/or yield of a plant in the presence of reduced synthetic nitrogen fertilizers are provided. Endophytic microbial strains serve as biocatalysts to solubilize organic (proteinaceous) nitrogen otherwise unavailable to plants for their nutritional needs. Thus defined, biocatalysts will serve to replace synthetic nitrogen fertilizers. Also provided are materials and methods for inoculating plants with these biocatalysts at carefully selected inoculum densities to reliably reduce the amount of nitrogen fertilizer by 50% thus accomplishing optimal yields in technically and cost-effective manner.

公开(公告)号：US09968642B2

公开(公告)日：2018-05-15

申请号：US14567751

申请日：2014-12-11

申请人： Northwestern University

发明人： Charles N. Rudick ,  David J. Klumpp ,  Anthony J. Schaeffer

IPC分类号： A61K35/741 ,  C12R1/19 ,  C12N1/20 ,  A61K35/00

CPC分类号： A61K35/741 ,  A61K2035/115 ,  C12N1/20 ,  C12R1/19 ,  Y02A50/473

摘要： The present invention provides compositions and methods for treatment or prevention of pain resulting from infection, infection related pain, and non-infectious pain as well as treatment or prevention of infections or adverse health consequences associated with infections.

公开(公告)号：US20170226541A1

公开(公告)日：2017-08-10

申请号：US15502747

申请日：2015-08-21

申请人： Nanyang Technological University ,  National University of Singapore

发明人： Jamie Hinks ,  Stefan Wuertz

IPC分类号： C12P7/16 ,  C12N1/04 ,  C12N15/01

CPC分类号： C12P7/16 ,  C12N1/04 ,  C12N15/01 ,  C12R1/19 ,  Y02E50/10

摘要： The invention relates generally to a method of improving solvent tolerance in microbes, and in particular, to a method of improving bio-butanol titre in a fermentation process due to improved solvent tolerance in microbes. The method of improving butanol titre in a fermentation process involves the addition of a membrane insertion molecule comprising a conjugated oligoelectrolyte, particularly an oligo-polyphenylene vinylene conjugated oligoelectrolyte.

公开(公告)号：US09677103B2

公开(公告)日：2017-06-13

申请号：US13966600

申请日：2013-08-14

申请人： Fujifilm Diosynth Biotechnologies UK Limited

发明人： Bhupendra Vallabh Kara ,  Christopher David John Lennon ,  Ian John Hodgson

IPC分类号： C12P21/00 ,  C12N15/70 ,  C12N15/72 ,  C12N15/78 ,  C12N15/81 ,  C12N15/85 ,  C12N15/73 ,  C12R1/19

CPC分类号： C12P21/00 ,  C12N15/70 ,  C12N15/72 ,  C12N15/73 ,  C12N15/78 ,  C12N15/81 ,  C12N15/85 ,  C12R1/19

摘要： A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.

公开(公告)号：US09315770B2

公开(公告)日：2016-04-19

申请号：US14827408

申请日：2015-08-17

申请人： UCB PHARMA, S.A.

发明人： Mark Ellis ,  David Paul Humphreys

IPC分类号： C12N1/21 ,  C12N1/20 ,  C12R1/19 ,  C07K16/24 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02

CPC分类号： C12N1/20 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/55 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19 ,  Y02P20/52

摘要： A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

公开(公告)号：US09125864B2

公开(公告)日：2015-09-08

申请号：US13702949

申请日：2011-06-10

申请人： Renata Maria Grifantini ,  Erika Bartolini ,  Oretta Finco ,  Guido Grandi

发明人： Renata Maria Grifantini ,  Erika Bartolini ,  Oretta Finco ,  Guido Grandi

IPC分类号： A61K39/118 ,  A61K45/00 ,  A61K39/02 ,  A61K39/385 ,  C12N1/20 ,  C12N1/00 ,  C12N1/12 ,  C07K14/245 ,  C07K14/295 ,  C12P21/02 ,  A61K39/00

CPC分类号： A61K39/118 ,  A61K2039/52 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/55505 ,  A61K2039/575 ,  A61K2039/6037 ,  A61K2039/6068 ,  C07K14/245 ,  C07K14/295 ,  C12N1/20 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19

摘要： The invention is in the field of outer membrane vesicles (OMV) and their uses. More particularly the present invention provides OMV obtained from a bacterium being an ompA mutant and/or a mutant in one or more components of the TolPal complex and presenting a heterologous antigen on its surface. The heterologous antigen is selected from the group consisting of Chlamydia trachomatis CT823, CT681, CT372, CT443, CT043, CT733, CT279, CT601 and CT153 for the treatment, prevention or diagnosis of Chlamydia infection.

摘要翻译： 本发明涉及外膜囊泡（OMV）及其用途。 更具体地说，本发明提供从在一个或多个TolPal复合物组分中的ompA突变体和/或突变体获得的OMV，并在其表面上呈递异源抗原。 异源抗原选自沙眼衣原体CT823，CT681，CT372，CT443，CT043，CT733，CT279，CT601和CT153，用于治疗，预防或诊断衣原体感染。