公开(公告)号：US20100167275A1

公开(公告)日：2010-07-01

申请号：US12121900

申请日：2008-05-16

申请人： Ekkehard Leberer ,  Thomas Leeuw ,  Allegra Ritscher

发明人： Ekkehard Leberer ,  Thomas Leeuw ,  Allegra Ritscher

IPC分类号： C12Q1/68 ,  C12Q1/02 ,  C12Q1/06 ,  C12N1/19

CPC分类号： C07K14/395

摘要： The invention relates to processes for identifying inhibitors and activators of eukaryotic potassium channels, in which a mutated S. cerevisiae cell is used whose endogenous potassium channels TRK1, TRK2 and TOK1 are not expressed functionally, but which expresses heterologously a eukaryotic potassium channel to be studied. Other subject matters of the invention are mutated S. cerevisiae cells which do not express TRK1, TRK2 and TOK1, and the preparation and use of these mutated S. cerevisiae cells.

摘要翻译： 本发明涉及用于鉴定真核钾通道的抑制剂和活化剂的方法，其中使用其内源性钾通道TRK1，TRK2和TOK1在功能上不表达但是在异源表达待研究的真核钾通道的突变型酿酒酵母细胞 。 本发明的其它主题是突变的不表达TRK1，TRK2和TOK1的酿酒酵母细胞，以及这些突变的酿酒酵母细胞的制备和使用。

公开(公告)号：US07429449B2

公开(公告)日：2008-09-30

申请号：US09758036

申请日：2001-01-11

申请人： Ekkehard Leberer ,  Thomas Leeuw ,  Allegra Ritscher

发明人： Ekkehard Leberer ,  Thomas Leeuw ,  Allegra Ritscher

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/569 ,  C12N1/00

CPC分类号： C07K14/395

摘要： The invention relates to processes for identifying inhibitors and activators of eukaryotic potassium channels, in which a mutated S. cerevisiae cell is used whose endogenous potassium channels TRK1, TRK2 and TOK1 are not expressed functionally, but which expresses heterologously a eukaryotic potassium channel to be studied. Other subject matters of the invention are mutated S. cerevisiae cells which do not express TRK1, TRK2 and TOK1, and the preparation and use of these mutated S. cerevisiae cells.

摘要翻译： 本发明涉及用于鉴定真核钾通道的抑制剂和活化剂的方法，其中使用其内源性钾通道TRK1，TRK2和TOK1在功能上不表达但是在异源表达待研究的真核钾通道的突变型酿酒酵母细胞 。 本发明的其它主题是突变的不表达TRK1，TRK2和TOK1的酿酒酵母细胞，以及这些突变的酿酒酵母细胞的制备和使用。