公开(公告)号：US20120060413A1

公开(公告)日：2012-03-15

申请号：US13233498

申请日：2011-09-15

申请人： Mariya Somleva ,  Himani Chinnapen ,  Aminat Ali ,  Kristi D. Snell ,  Oliver P. Peoples ,  Nii Patterson ,  Jihong Tang ,  Karen Bohmert-Tatarev

发明人： Mariya Somleva ,  Himani Chinnapen ,  Aminat Ali ,  Kristi D. Snell ,  Oliver P. Peoples ,  Nii Patterson ,  Jihong Tang ,  Karen Bohmert-Tatarev

IPC分类号： C10L8/00 ,  C12N5/10 ,  C08L67/04 ,  C12N15/82 ,  C08L97/02 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8261 ,  C10B53/02 ,  C10G3/00 ,  C10G2300/1014 ,  C10J2300/0916 ,  C10J2300/092 ,  C10L5/44 ,  C12N15/8243 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8269 ,  C12P7/625 ,  Y02A40/146 ,  Y02E50/10 ,  Y02E50/30 ,  Y02P30/20

摘要： Transgenic plants, transgenic plant material, and transgenic plant cells for the improved synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to as PHB), have been developed. In one embodiment, carbon flow is modulated to increase production of PHB. Preferred plants that can be genetically engineered to produce PHB include plants that produce a large amount of lignocellulosic biomass that can be converted into biofuels, such as switchgrass, Miscanthus, Sorghum, sugarcane, millets, Napier grass and other forage and turf grasses. An exemplary plant that can be genetically engineered to produce PHB and produces lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include, but are not limited to, Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

摘要翻译： 已经开发了转基因植物，转基因植物材料和用于改进合成聚羟基链烷酸酯，优选聚（3-羟基丁酸酯）（也称为PHB））的转基因植物细胞。 在一个实施方案中，调节碳流以增加PHB的产生。 可以遗传工程化生产PHB的优选植物包括产生大量木质纤维素生物质的植物，其可以转化成生物燃料，例如柳枝稷，芒草，高粱，甘蔗，米勒，纳皮尔草和其他草料和草坪草。 可以进行基因工程以生产PHB并产生木质纤维素生物质的示范性植物是柳枝稷（Panicum virgatum L.）。优选的柳枝稷品种是Alamo。 柳枝稷的其他合适品种包括但不限于：Blackwell，Kanlow，Nebraska 28，Pathfinder，Cave-in-Rock，Shelter和Trailblazer。

公开(公告)号：US20100229256A1

公开(公告)日：2010-09-09

申请号：US12718559

申请日：2010-03-05

申请人： Mariya Somleva ,  Aminat Ali

发明人： Mariya Somleva ,  Aminat Ali

IPC分类号： A01H4/00 ,  A01H5/00

CPC分类号： A01H4/005 ,  C12N15/8257

摘要： Methods for increasing the product yield from plants, preferably transgenic plants, are provided. It has been discovered that in vitro cultures from donor plants produce plants that have two or three fold increase in product yield. One embodiment provides a method for increasing product yield from a transgenic plant by initiating an in vitro culture from a donor transgenic plant, wherein the donor transgenic plant is genetically engineered to produce a product and regenerating a second transgenic plant from the in vitro culture, wherein the yield of the product from the second transgenic plant is greater than the yield of the product from the donor transgenic plant. In a preferred embodiment, the transgenic plant is a graminaceous plant such as switchgrass.

摘要翻译： 提供了从植物，优选转基因植物提高产品产量的方法。 已经发现，来自供体植物的体外培养产生的产品产量增加了两到三倍的植物。 一个实施方案提供了通过从供体转基因植物开始体外培养来提高来自转基因植物的产量的方法，其中所述供体转基因植物被遗传工程化以产生产物并从体外培养物再生第二转基因植物，其中 来自第二转基因植物的产物的产量大于来自供体转基因植物的产物的产量。 在优选的实施方案中，转基因植物是诸如柳枝稷的禾本科植物。