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1.发明授权Stimulated and coherent anti-stokes raman spectroscopic methods for the detection of molecules 有权刺激和连贯的反斯托克斯拉曼光谱法检测分子公开(公告)号：US08088628B2
公开(公告)日：2012-01-03
申请号：US10262349
申请日：2002-09-30
IPC分类号： G01N21/03
CPC分类号： G01N21/65 , G01N2021/052 , G01N2021/653 , G01N2021/655 , Y10T436/143333 , Y10T436/24
摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed which exploit inelastically scattering radiation to amplify optical signals from an irradiated sample. Samples are irradiated in a chamber having a resonant cavity containing a plurality of affixed reflectors, where selective Stokes scattered radiation is transmitted to a detector for determination of sample identity. Coupling the spectroscopic analysis system and method with a nucleic acid sequencing system is also disclosed for determining nucleic acid sequences.
摘要翻译： 公开了用于分析样品的光谱分析系统和方法，其利用非常散射的辐射来放大来自辐射样品的光信号。 在具有包含多个固定反射器的谐振腔的腔室中照射样品，其中选择性斯托克斯散射辐射被传输到检测器以确定样品身份。 还公开了用于测定核酸序列的光谱分析系统和方法与核酸测序系统的偶联。
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公开(公告)号：US08009288B2
公开(公告)日：2011-08-30
申请号：US11404680
申请日：2006-04-14
CPC分类号： G01N21/65 , G01N21/658 , G01N2021/052 , G01N2021/653 , G01N2021/655 , Y10T436/143333
摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.
摘要翻译： 公开了用于分析样品的光谱分析系统和方法。 分析系统可以包含用于提供辐射的电磁辐射源，用于执行相干拉曼光谱（例如，受激拉曼或相干反斯托克斯拉曼光谱）的光谱分析室，以及基于光谱检测辐射的辐射检测器。 腔室可以具有一个谐振腔，以容纳用于分析的样本，至少一个窗口，用于将腔室中的第一辐射传输到腔体中并将第二辐射透射出来，多个反射器固定到空腔的壳体以反射 预定频率的辐射，多个反射器分开足以使辐射共振的距离。 光谱分析系统可以与核酸测序系统结合以在溶液中接受单个核酸衍生物，并鉴定衍生物以对核酸进行排序。
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公开(公告)号：US20100267013A1
公开(公告)日：2010-10-21
申请号：US11753361
申请日：2007-05-24
申请人： Xing Su , Andrew Arthur Berlin , Selena Chan , Steven J. Kirch , Tae-Woong Koo , Gabi Neubauer , Valluri Rao , Narayan Sundararajan , Mineo Yamakawa
发明人： Xing Su , Andrew Arthur Berlin , Selena Chan , Steven J. Kirch , Tae-Woong Koo , Gabi Neubauer , Valluri Rao , Narayan Sundararajan , Mineo Yamakawa
CPC分类号： C12Q1/6869 , C12Q2565/632 , C12Q2563/155 , C12Q2521/319
摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
摘要翻译： 本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。 在本发明的某些实施方案中，在掺入核酸之前，核苷酸与拉曼标记物共价连接。 在其它实施方案中，使用未标记的核酸。 核酸外切核酸处理导致通过拉曼光谱法检测的标记或未标记的核苷酸的释放。 在本发明的替代实施方案中，通过外切核酸酶处理从核酸释放的核苷酸与纳米颗粒共价交联，并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干反斯托克斯拉曼 光谱学（CARS）。 本发明的其它实施方案涉及用于核酸测序的装置。
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公开(公告)号：US20110275144A1
公开(公告)日：2011-11-10
申请号：US13185712
申请日：2011-07-19
IPC分类号： C12M1/34
CPC分类号： G01N21/65 , G01N21/658 , G01N2021/052 , G01N2021/653 , G01N2021/655 , Y10T436/143333
摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.
摘要翻译： 公开了用于分析样品的光谱分析系统和方法。 分析系统可以包含用于提供辐射的电磁辐射源，用于执行相干拉曼光谱（例如，受激拉曼或相干反斯托克斯拉曼光谱）的光谱分析室，以及基于光谱检测辐射的辐射检测器。 腔室可以具有一个谐振腔，以容纳用于分析的样本，至少一个窗口，用于将腔室中的第一辐射传输到腔体中并将第二辐射透射出来，多个反射器固定到空腔的壳体以反射 预定频率的辐射，多个反射器分开足以使辐射共振的距离。 光谱分析系统可以与核酸测序系统结合以在溶液中接受单个核酸衍生物，并鉴定衍生物以对核酸进行排序。
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公开(公告)号：US08513002B2
公开(公告)日：2013-08-20
申请号：US13185712
申请日：2011-07-19
IPC分类号： C12M1/34 , G01N33/00 , G01N33/553 , C12Q1/68 , C12M3/00
CPC分类号： G01N21/65 , G01N21/658 , G01N2021/052 , G01N2021/653 , G01N2021/655 , Y10T436/143333
摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.
摘要翻译： 公开了用于分析样品的光谱分析系统和方法。 分析系统可以包含用于提供辐射的电磁辐射源，用于执行相干拉曼光谱（例如，受激拉曼或相干反斯托克斯拉曼光谱）的光谱分析室，以及基于光谱检测辐射的辐射检测器。 腔室可以具有一个谐振腔，以容纳用于分析的样本，至少一个窗口，用于将腔室中的第一辐射传输到腔体中并将第二辐射透射出来，多个反射器固定到空腔的壳体以反射 预定频率的辐射，多个反射器分开足以使辐射共振的距离。 光谱分析系统可以与核酸测序系统结合以在溶液中接受单个核酸衍生物，并鉴定衍生物以对核酸进行排序。
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公开(公告)号：US20120034686A1
公开(公告)日：2012-02-09
申请号：US13220214
申请日：2011-08-29
IPC分类号： C12M1/34
CPC分类号： G01N21/65 , G01N21/658 , G01N2021/052 , G01N2021/653 , G01N2021/655 , Y10T436/143333
摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.
摘要翻译： 公开了用于分析样品的光谱分析系统和方法。 分析系统可以包含用于提供辐射的电磁辐射源，用于执行相干拉曼光谱（例如，受激拉曼或相干反斯托克斯拉曼光谱）的光谱分析室，以及基于光谱检测辐射的辐射检测器。 腔室可以具有一个谐振腔，以容纳用于分析的样本，至少一个窗口，用于将腔室中的第一辐射传输到腔体中并将第二辐射透射出来，多个反射器固定到空腔的壳体以反射 预定频率的辐射，多个反射器分开足以使辐射共振的距离。 光谱分析系统可以与核酸测序系统结合以在溶液中接受单个核酸衍生物，并鉴定衍生物以对核酸进行排序。
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