公开(公告)号：US08124841B2

公开(公告)日：2012-02-28

申请号：US12603670

申请日：2009-10-22

申请人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng Zhang ,  Xuejun Zhong

发明人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng Zhang ,  Xuejun Zhong

IPC分类号： C12N15/82 ,  C12N15/56 ,  C12N5/04 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8257 ,  C12N9/2414 ,  C12N9/2422 ,  C12N15/8242 ,  C12N15/8245

摘要： The present invention is directed to C-terminal truncated α-amylase polypeptides, nucleic acid sequences encoding the truncated α-amylase polypeptides, and methods of using the same. Further provided are expression cassettes, plants and plant parts expressing the nucleic acid sequence encoding the truncated α-amylase. Expression of the truncated α-amylase in a plant cell results in enhanced accumulation of biologically-active α-amylase, or enhanced secretion of the α-amylase from the plant cell, compared to the accumulation or secretion of a full length α-amylase.

摘要翻译： 本发明涉及C末端截短的α-淀粉酶多肽，编码截短的α-淀粉酶多肽的核酸序列及其使用方法。 还提供了表达编码截短的α-淀粉酶的核酸序列的表达盒，植物和植物部分。 与全长α-淀粉酶的积累或分泌相比，截短的α-淀粉酶在植物细胞中的表达导致生物活性α-淀粉酶的积累增加，或来自植物细胞的α-淀粉酶的分泌增加。

公开(公告)号：US20100100983A1

公开(公告)日：2010-04-22

申请号：US12603670

申请日：2009-10-22

申请人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng ZHANG ,  Xuejun Zhong

发明人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng ZHANG ,  Xuejun Zhong

IPC分类号： A01H1/00 ,  C12N5/10 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8257 ,  C12N9/2414 ,  C12N9/2422 ,  C12N15/8242 ,  C12N15/8245

摘要： The present invention is directed to C-terminal truncated α-amylase polypeptides, nucleic acid sequences encoding the truncated α-amylase polypeptides, and methods of using the same. Further provided are expression cassettes, plants and plant parts expressing the nucleic acid sequence encoding the truncated α-amylase. Expression of the truncated α-amylase in a plant cell results in enhanced accumulation of biologically-active α-amylase, or enhanced secretion of the α-amylase from the plant cell, compared to the accumulation or secretion of a full length α-amylase.

摘要翻译： 本发明涉及C末端截短的α-淀粉酶多肽，编码截短的α-淀粉酶多肽的核酸序列及其使用方法。 还提供了表达编码截短的α-淀粉酶的核酸序列的表达盒，植物和植物部分。 与全长α-淀粉酶的积累或分泌相比，截短的α-淀粉酶在植物细胞中的表达导致生物活性α-淀粉酶的积累增加，或来自植物细胞的α-淀粉酶的分泌增加。

公开(公告)号：US20120185969A1

公开(公告)日：2012-07-19

申请号：US13393846

申请日：2010-09-02

申请人： Andrew DeBrecht ,  Kasimalai Azhakanandam

发明人： Andrew DeBrecht ,  Kasimalai Azhakanandam

IPC分类号： A01H5/00 ,  C12N5/10 ,  A01H5/10 ,  C12N15/82

CPC分类号： C12N15/8216

摘要： Compositions and methods for increasing expression of a polypeptide of interest in a plant or plant part thereof are provided. Compositions of the invention are polynucleotide constructs comprising (a) at least one translational enhancer element derived from a virus tandemly stacked with at least one translational enhancer element derived from a cellular gene, and (b) an operably linked polynucleotide encoding a polypeptide of interest. Expression cassettes, vectors, and transgenic plants and plant parts comprising these polynucleotide constructs are also provided. Methods for increasing expression of a polypeptide of interest in a plant or plant part thereof utilizing the polynucleotide constructs and expression cassettes of the invention are also provided.

摘要翻译： 提供了用于增加植物或植物部分中感兴趣的多肽的表达的组合物和方法。 本发明的组合物是多核苷酸构建体，其包含（a）至少一种衍生自与来自细胞基因的至少一种翻译增强子串联堆叠的病毒的翻译增强子元件，和（b）编码目的多肽的可操作地连接的多核苷酸。 还提供了表达盒，载体和包含这些多核苷酸构建体的转基因植物和植物部分。 还提供了利用本发明的多核苷酸构建体和表达盒在其植物或植物部分中增加目的多肽表达的方法。