公开(公告)号：US08124841B2

公开(公告)日：2012-02-28

申请号：US12603670

申请日：2009-10-22

申请人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng Zhang ,  Xuejun Zhong

发明人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng Zhang ,  Xuejun Zhong

IPC分类号： C12N15/82 ,  C12N15/56 ,  C12N5/04 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8257 ,  C12N9/2414 ,  C12N9/2422 ,  C12N15/8242 ,  C12N15/8245

摘要： The present invention is directed to C-terminal truncated α-amylase polypeptides, nucleic acid sequences encoding the truncated α-amylase polypeptides, and methods of using the same. Further provided are expression cassettes, plants and plant parts expressing the nucleic acid sequence encoding the truncated α-amylase. Expression of the truncated α-amylase in a plant cell results in enhanced accumulation of biologically-active α-amylase, or enhanced secretion of the α-amylase from the plant cell, compared to the accumulation or secretion of a full length α-amylase.

摘要翻译： 本发明涉及C末端截短的α-淀粉酶多肽，编码截短的α-淀粉酶多肽的核酸序列及其使用方法。 还提供了表达编码截短的α-淀粉酶的核酸序列的表达盒，植物和植物部分。 与全长α-淀粉酶的积累或分泌相比，截短的α-淀粉酶在植物细胞中的表达导致生物活性α-淀粉酶的积累增加，或来自植物细胞的α-淀粉酶的分泌增加。

公开(公告)号：US20100100983A1

公开(公告)日：2010-04-22

申请号：US12603670

申请日：2009-10-22

申请人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng ZHANG ,  Xuejun Zhong

发明人： Andrew DeBrecht ,  Aron Silverstone ,  Yan Zhang ,  Shengsheng ZHANG ,  Xuejun Zhong

IPC分类号： A01H1/00 ,  C12N5/10 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8257 ,  C12N9/2414 ,  C12N9/2422 ,  C12N15/8242 ,  C12N15/8245

摘要： The present invention is directed to C-terminal truncated α-amylase polypeptides, nucleic acid sequences encoding the truncated α-amylase polypeptides, and methods of using the same. Further provided are expression cassettes, plants and plant parts expressing the nucleic acid sequence encoding the truncated α-amylase. Expression of the truncated α-amylase in a plant cell results in enhanced accumulation of biologically-active α-amylase, or enhanced secretion of the α-amylase from the plant cell, compared to the accumulation or secretion of a full length α-amylase.

摘要翻译： 本发明涉及C末端截短的α-淀粉酶多肽，编码截短的α-淀粉酶多肽的核酸序列及其使用方法。 还提供了表达编码截短的α-淀粉酶的核酸序列的表达盒，植物和植物部分。 与全长α-淀粉酶的积累或分泌相比，截短的α-淀粉酶在植物细胞中的表达导致生物活性α-淀粉酶的积累增加，或来自植物细胞的α-淀粉酶的分泌增加。

公开(公告)号：US20110039308A1

公开(公告)日：2011-02-17

申请号：US12520523

申请日：2007-12-21

申请人： Malgorzata Slupska ,  Geoff Hazlewood ,  Cathy Chang ,  Peter Luginbuhl ,  Ellen Burke ,  Michelle Cayouette ,  Uvini Gunavardena ,  Majid Ghassemian ,  Aron Silverstone ,  Yan Zhang

发明人： Malgorzata Slupska ,  Geoff Hazlewood ,  Cathy Chang ,  Peter Luginbuhl ,  Ellen Burke ,  Michelle Cayouette ,  Uvini Gunavardena ,  Majid Ghassemian ,  Aron Silverstone ,  Yan Zhang

IPC分类号： C12P19/04 ,  C10L1/00 ,  A23L1/28 ,  C12P7/04 ,  C12P7/16 ,  C12P7/06 ,  C12N9/26 ,  C12N9/34 ,  C12N5/10 ,  C12N15/63 ,  C40B50/00 ,  C07H21/04 ,  C10L1/224

CPC分类号： C12N9/96 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2425 ,  C12N9/2428 ,  Y02E50/16 ,  Y02E50/17

摘要： In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

摘要翻译： 一方面，本发明涉及具有淀粉酶和/或葡糖淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化多糖，寡糖或淀粉水解成糖。 一方面，本发明提供包含通过胶乳聚合物涂层涂覆的所需成分的延迟释放组合物。 在替代实施方案中，酶用于制备生物燃料，例如乙醇，丁醇，丙醇或汽油 - 乙醇混合物，包括生物乙醇，生物丙醇，生物丁醇或生物柴油，或用于任何形式的燃料或生物质处理。