公开(公告)号：US20120276247A1

公开(公告)日：2012-11-01

申请号：US13098124

申请日：2011-04-29

申请人： Uwe Bornscheuer ,  Henrike Brundiek ,  Andrew Evitt ,  Stefan Sass ,  Friedericke Bönisch ,  Robert Kourist

发明人： Uwe Bornscheuer ,  Henrike Brundiek ,  Andrew Evitt ,  Stefan Sass ,  Friedericke Bönisch ,  Robert Kourist

IPC分类号： C12N9/20 ,  C12N9/02 ,  C12N15/57 ,  C12N15/63 ,  A23D9/00 ,  C12N15/81 ,  C12N1/21 ,  C12N1/19 ,  A23D9/04 ,  C12N11/02 ,  C12N15/70

CPC分类号： C12N9/20 ,  A23D9/04 ,  C12P7/6454

摘要： The present invention relates to a newly identified lipase belonging to the Ustilaginaceae family of Basidomycetes and variants thereof. The invention also relates to polynucleotides encoding the lipase and the variants thereof. The invention further relates to procedures for producing the lipase, and variants thereof, polypeptides and polynucleotides. The invention further relates to lipase variants with an increased trans-selectivity. The invention further relates to lipase variants with a preference for long chain fatty acid esters or carboxylic acid moieties with a chain length greater than C12. The invention further relates to a method of reducing, or eliminating, trans-fatty acids from liquid oil stable enough for frying systems.

摘要翻译： 本发明涉及属于Basidomycetes的Ustilaginaceae科的新鉴定的脂肪酶及其变体。 本发明还涉及编码脂肪酶的多核苷酸及其变体。 本发明还涉及产生脂肪酶的方法及其变体，多肽和多核苷酸。 本发明还涉及具有增加的反式选择性的脂肪酶变体。 本发明还涉及优选长链脂肪酸酯或链长大于C12的羧酸部分的脂肪酶变体。 本发明还涉及一种减少或消除来自液体油的反式脂肪酸足以用于煎炸系统的方法。