公开(公告)号：US20090263406A1

公开(公告)日：2009-10-22

申请号：US12258800

申请日：2008-10-27

申请人： Jianbo Yao ,  George W. Smith ,  Anilkumar Bettegowda ,  Paul M. Coussens ,  James J. Ireland

发明人： Jianbo Yao ,  George W. Smith ,  Anilkumar Bettegowda ,  Paul M. Coussens ,  James J. Ireland

IPC分类号： A61K39/00 ,  C07K14/00 ,  C07K16/00 ,  C07H21/02 ,  A61K31/7088 ,  A61K38/16 ,  A61P15/00

CPC分类号： C07K14/47 ,  A61K38/00 ,  C07K16/18 ,  C12N5/0604 ,  C12N2501/998

摘要： The present invention provides compositions and methods for regulating fertility in mammals. In general, the invention relates to a novel protein produced by oocytes named JY-1, and nucleic acids encoding the JY-1 protein, for controlling folliculogenesis and early embryonic development, particularly in monoovulatory species. In particular, the present invention provides nucleic acid and amino acid sequences encoding JY-1, vectors for the expression of JY-1, host cells expressing JY-1, RNAi probes for reducing levels of JY-1 message, and antibodies to JY-1. Specifically, developing and mature oocytes express JY-1 in vivo, while granulosa cells treated in vitro with recombinant JY-1 (rJY-1) protein reduced cell proliferation while increasing progesterone synthesis and estradiol production. Further, reducing JY-1 protein in developing embryos in vitro using inhibitory siRNA constructs corresponded with arrested blastocyte maturation.

摘要翻译： 本发明提供了调节哺乳动物生育力的组合物和方法。 通常，本发明涉及由命名为JY-1的卵母细胞产生的新型蛋白质，以及编码JY-1蛋白质的核酸，用于控制卵泡发生和早期胚胎发育，特别是单调节物种。 特别是本发明提供编码JY-1的核酸和氨基酸序列，JY-1表达载体，JY-1表达宿主细胞，用于降低JY-1信号水平的RNAi探针，JY- 1。 具体来说，开发和成熟的卵母细胞在体内表达JY-1，而用重组JY-1（rJY-1）蛋白体外处理的颗粒细胞减少细胞增殖，同时增加孕酮合成和雌二醇生产。 此外，使用抑制性siRNA构建体在体外发育胚胎中减少JY-1蛋白质对应于被阻止的胚细胞成熟。